Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	148	57.0	1973990	96.6%	1906874	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,004,562	A→G	S206G (AGC→GGC)	ycbF →	putative fimbrial chaperone YcbF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,004,562	0	A	G	100.0%	157.6 / NA	45	S206G (AGC→GGC)	ycbF	putative fimbrial chaperone YcbF
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/23); total (22/23)

CGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGT  >  NC_000913/1004427‑1004697

cGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGc                                                                                                                                        >  1:202939/1‑138 (MQ=255)

              ttCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCg                                                                                                                        <  2:535564/140‑1 (MQ=255)

                cGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      <  2:902527/140‑1 (MQ=255)

                cGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      <  2:822860/140‑1 (MQ=255)

                cGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      <  1:623585/140‑1 (MQ=255)

                      gtgtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      <  2:983453/134‑1 (MQ=255)

                      gtgtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCa                                                                                                                      >  1:983453/1‑134 (MQ=255)

                        gtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCg                                                                                                                   >  2:545194/1‑135 (MQ=255)

                        gtACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCg                                                                                                                   <  1:545194/135‑1 (MQ=255)

                         tACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCAt                                                                                                             <  1:436314/140‑1 (MQ=255)

                               tCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAACCGCACCGTGTCAt                                                                                                             >  1:378700/1‑134 (MQ=255)

                               tCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAACCGCACCGTGTCAt                                                                                                             <  2:378700/134‑1 (MQ=255)

                                 aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTg                                                                                                     >  1:341041/1‑140 (MQ=255)

                                 aCTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTg                                                                                                     >  2:471762/1‑140 (MQ=255)

                                      cccAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTggcgtg                                                                                                >  1:397954/1‑138 (MQ=255)

                                      cccAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTggcggg                                                                                                >  1:28496/1‑140 (MQ=255)

                                          aCGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGAt                                                                                            >  2:115639/1‑140 (MQ=255)

                                           cGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATa                                                                                           <  2:461454/140‑1 (MQ=255)

                                                 attaCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGAt                                                                                     >  2:378605/1‑140 (MQ=255)

                                                 attaCATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGAt                                                                                     >  1:768903/1‑140 (MQ=255)

                                                        tAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTAcggcg                                                                              >  1:279285/1‑140 (MQ=255)

                                                          aCCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCggg                                                                            >  1:711236/1‑140 (MQ=255)

                                                            cTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGtt                                                                          <  1:115639/140‑1 (MQ=255)

                                                            cTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGtt                                                                          >  2:451329/1‑140 (MQ=255)

                                                            cTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGtt                                                                          <  2:279285/140‑1 (MQ=255)

                                                            cTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGtt                                                                          <  2:188309/140‑1 (MQ=255)

                                                               aTTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAg                                                                       >  2:539133/1‑140 (MQ=255)

                                                                    ggTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTAcg                                                                                 <  1:134252/125‑1 (MQ=255)

                                                                    ggTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTAcg                                                                                 >  2:134252/1‑125 (MQ=255)

                                                                    ggTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGCaa                                                                  >  1:910462/1‑140 (MQ=255)

                                                                                  gTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGTGGGGGAAGAAGGA‑GGaaaaa                                                   >  2:647107/1‑139 (MQ=255)

                                                                                   tAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATc                                                  <  1:554241/140‑1 (MQ=255)

                                                                                   tAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATc                                                  <  1:554369/140‑1 (MQ=255)

                                                                                   tAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATc                                                  <  1:471762/140‑1 (MQ=255)

                                                                                    aaaGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGAGGGGGAAAAAAGA‑GCgaaaaaa                                                 >  2:11622/1‑137 (MQ=255)

                                                                                      aGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGc                                               <  1:464040/140‑1 (MQ=255)

                                                                                          ttAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGGGTGGGAAAAAAGA‑GGAAAAACAGccccc                                           >  2:507536/1‑138 (MQ=255)

                                                                                             aGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGaaa                                        <  1:118797/140‑1 (MQ=255)

                                                                                                  tgtgGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGGGTGGAAAGAAAGAGGCAAAAACTCCCCGTAAAAAgg                                    >  1:378019/1‑140 (MQ=255)

                                                                                                      ggAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGGTGTGTGGCAAGAAAGA‑GCAAAAACATCCCTCAAAAAAGGTCgg                               >  1:228552/1‑140 (MQ=255)

                                                                                                                         gCCAGCGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGttttttt            <  1:799883/140‑1 (MQ=255)

                                                                                                                              cGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCg       <  1:647107/140‑1 (MQ=255)

                                                                                                                              cGTCAGACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCg       <  1:539133/140‑1 (MQ=255)

                                                                                                                                   gACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGt  <  1:553089/140‑1 (MQ=255)

                                                                                                                                   gACAGGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGt  <  1:11622/140‑1 (MQ=255)

CGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTATTACATTAACCTGATTCAGGTGAGTGTAAATGGTAAAGCGTTAAGTAATGTGGGAGTTGTGCCGCCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCAATCGCACCGTGTCATGTCGCCTGGCGGGCGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGA‑GCAAAATCTGCCCTGAAACAGGTTCGGAAAACGTTTGCGTTTTTTTTGCCGCAGGT  >  NC_000913/1004427‑1004697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A