Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	148	57.0	1973990	96.6%	1906874	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,177,187	A→G	E190G (GAA→GGA)	lolD →	lipoprotein release complex ‑ ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,177,187	0	A	G	97.6%	136.5 / ‑4.9	42	E190G (GAA→GGA)	lolD	lipoprotein release complex ‑ ATP binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (19/22); total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTA  >  NC_000913/1177057‑1177319

tCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCg                                                                                                                             >  1:765365/1‑140 (MQ=255)

 cTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGc                                                                                                                            <  2:117927/140‑1 (MQ=255)

          aaCGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGc                                                                                                                   >  1:172923/1‑140 (MQ=255)

              ccAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCg                                                                                                               <  1:819147/140‑1 (MQ=255)

              ccAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCg                                                                                                               <  1:762087/140‑1 (MQ=255)

               cAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGc                                                                                                              <  2:170529/140‑1 (MQ=255)

                    tgtgGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTcc                                                                                                         <  2:765365/140‑1 (MQ=255)

                    tgtgGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTcc                                                                                                         <  1:872507/140‑1 (MQ=255)

                      tgGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCtg                                                                                                       >  2:824820/1‑140 (MQ=255)

                      tgGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCtg                                                                                                       <  1:966138/140‑1 (MQ=255)

                       ggCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCtgg                                                                                                      >  1:666533/1‑140 (MQ=255)

                           aTTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTggtggt                                                                                                  <  1:384155/140‑1 (MQ=255)

                             tGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTa                                                                                                >  1:94056/1‑140 (MQ=255)

                              gCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTAc                                                                                               >  1:23640/1‑140 (MQ=255)

                                 cGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCa                                                                                            <  2:336392/140‑1 (MQ=255)

                                        tGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCtggtgg                                                                                                   >  1:968906/1‑126 (MQ=255)

                                        tGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCtggtgg                                                                                                   <  2:968906/126‑1 (MQ=255)

                                          gTGAATAACCCGCGCCTGGTACTGGCGGAAGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCCGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCa                                                                                   >  2:711454/1‑140 (MQ=255)

                                                  cccGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGa                                                                           <  2:94056/140‑1 (MQ=255)

                                                     gcgcCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAAc                                                                        >  1:207289/1‑140 (MQ=255)

                                                         cTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTAt                                                                    >  1:892945/1‑140 (MQ=255)

                                                             tACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAgc                                                                <  2:148877/140‑1 (MQ=255)

                                                               cTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAgccg                                                              <  2:432953/140‑1 (MQ=255)

                                                               cTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAgccg                                                              <  2:254534/140‑1 (MQ=255)

                                                                 ggCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAgccgcc                                                            >  1:409582/1‑140 (MQ=255)

                                                                 ggCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAgccgcc                                                            <  1:711027/140‑1 (MQ=255)

                                                                   cGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAg                                                          <  2:172923/140‑1 (MQ=255)

                                                                       tGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAg                                                      >  2:491336/1‑140 (MQ=255)

                                                                                     aCCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGgt                                        >  2:107721/1‑140 (MQ=255)

                                                                                     aCCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGgt                                        >  2:770781/1‑140 (MQ=255)

                                                                                     aCCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGgt                                        >  2:172410/1‑140 (MQ=255)

                                                                                        tCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGgtcgt                                     >  1:708761/1‑140 (MQ=255)

                                                                                            tGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGa                                 >  1:355603/1‑140 (MQ=255)

                                                                                                cgTAACGCCGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGAcggc                             >  2:199682/1‑140 (MQ=255)

                                                                                                       ccGACAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTg                      <  1:199682/140‑1 (MQ=255)

                                                                                                          aCAGCATCTTCCAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGc                   <  2:409582/140‑1 (MQ=255)

                                                                                                                    ccAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATggggg         <  1:824820/140‑1 (MQ=255)

                                                                                                                    ccAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATggggg         <  1:172410/140‑1 (MQ=255)

                                                                                                                    ccAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATggggg         <  1:491336/140‑1 (MQ=255)

                                                                                                                    ccAGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATggggg         <  1:165256/140‑1 (MQ=255)

                                                                                                                      aGTTGCTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCg       <  2:23640/140‑1 (MQ=255)

                                                                                                                           cTTGGGGGATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTa  >  1:317332/1‑140 (MQ=255)

TCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCGGTAACCTCGATGCGCGTAACGCCGACAGCATCTTCCAGTTGCTTGGGGAATTGAATCGCTTGCAGGGCACCGCCTTCCTGGTGGTTACTCACGACCTGCAACTGGCGAAACGTATGAGCCGCCAGTTAGAAATGCGTGATGGTCGTCTGACGGCGGAACTGAGCCTGATGGGGGCGGAGTA  >  NC_000913/1177057‑1177319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A