Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F28 I1 R1 95 36.3 1231394 98.7% 1215385 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 881,516 (G)5→4 coding (790/1203 nt) dacC → D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913881,5120G.100.0% 71.3 / NA 18coding (786/1203 nt)dacCD‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (9/9);  total (9/9)

GTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTG  >  NC_000913/881373‑881642
                                                                                                                                           |                                                                                                                                  
gTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTg                                                                                                                                    >  1:580081/1‑140 (MQ=255)
           cgtaaccgtCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAacc                                                                                                                        <  2:540168/140‑1 (MQ=255)
               accgtCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAaccgacc                                                                                                                    >  1:156274/1‑140 (MQ=255)
                    ctgctgTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGATTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTCGTGTT‑GGGGCGAAAACCGACCGTATc                                                                                                               >  2:347875/1‑140 (MQ=255)
                              gcagcaATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATg                                                                                                     <  2:251198/140‑1 (MQ=255)
                                  caATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTc                                                                                                 <  2:237497/140‑1 (MQ=255)
                                       tGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTgaga                                                                                            >  2:284300/1‑140 (MQ=255)
                                                aTGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGa                                                                                   >  1:74847/1‑140 (MQ=255)
                                                              ggAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGc                                                                     <  2:555166/140‑1 (MQ=255)
                                                                  ccACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGcttct                                                                 <  2:580081/140‑1 (MQ=255)
                                                                            cgcgGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGt                                                       <  1:547654/140‑1 (MQ=255)
                                                                                ggATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACg                                                   <  2:156274/140‑1 (MQ=255)
                                                                                           ggTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAAcc                                        >  1:225197/1‑140 (MQ=255)
                                                                                           ggTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAAcc                                        >  1:397724/1‑140 (MQ=255)
                                                                                              tGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGa                                     <  2:74847/140‑1 (MQ=255)
                                                                                                                   tatGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAgcg                >  2:281568/1‑140 (MQ=255)
                                                                                                                   tatGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAgcg                >  2:538360/1‑140 (MQ=255)
                                                                                                                   tatGCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAgcg                >  2:90488/1‑140 (MQ=255)
                                                                                                                      gCGTTTAATCTCCGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGt             <  2:225197/140‑1 (MQ=255)
                                                                                                                                 ccGTAGTGTT‑GGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTg  >  2:235634/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                  
GTCAGCCTAACCGTAACCGTCTGCTGTGGAGCAGCAATCTGAATGTTGATGGCATGAAGACAGGAACCACGGCAGGCGCGGGATATAATCTGGTTGCTTCGGCTACCCAGGGCGATATGCGTTTAATCTCCGTAGTGTTGGGGGCGAAAACCGACCGTATCCGTTTTAATGAGTCTGAGAAATTATTGACCTGGGGTTTCCGCTTCTTTGAAACCGTGACGCCAATTAAACCTGATGCCACCTTTGTGACTCAGCGCGTCTGGTTTGGTG  >  NC_000913/881373‑881642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A