Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F28 I1 R1
|
95 |
36.3 |
1231394 |
98.7% |
1215385 |
138.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,541,351 |
C→T |
E414K (GAA→AAA) |
narZ ← |
nitrate reductase Z subunit alpha |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,541,351 | 0 | C | T | 100.0%
| 83.6
/ NA
| 25 | E414K (GAA→AAA) | narZ | nitrate reductase Z subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (12/13); total (12/13) |
TTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCT > NC_000913/1541229‑1541486
|
ttGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACcggcgg > 1:459577/1‑140 (MQ=255)
cgcACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAAt < 1:429365/140‑1 (MQ=255)
cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCa < 1:577632/136‑1 (MQ=255)
cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCa > 2:577632/1‑136 (MQ=255)
gATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgattccagattc > 2:267302/1‑140 (MQ=255)
tgtgTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAt < 1:463902/140‑1 (MQ=255)
ttttACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGc > 1:129960/1‑140 (MQ=255)
aGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGc < 2:520750/140‑1 (MQ=255)
aGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt < 2:459577/140‑1 (MQ=255)
gAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCgg > 1:540864/1‑132 (MQ=255)
gAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCgg < 2:540864/132‑1 (MQ=255)
aaGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCaa < 1:267302/140‑1 (MQ=255)
ggCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAtt < 1:148982/140‑1 (MQ=255)
ggCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAtt > 2:291754/1‑140 (MQ=255)
gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg < 1:427168/140‑1 (MQ=255)
gCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGt < 1:291754/140‑1 (MQ=255)
cGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg > 2:96062/1‑129 (MQ=255)
cGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg < 1:96062/129‑1 (MQ=255)
gTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGGTTCCAGGTTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTTGGCATTACCAATTCACCTTCGGGATTAACTg > 2:520734/1‑140 (MQ=255)
gTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTg > 2:517222/1‑140 (MQ=255)
aTGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTa < 1:505128/140‑1 (MQ=255)
gAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTcc > 2:2708/1‑140 (MQ=255)
cAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACt < 1:517222/140‑1 (MQ=255)
ttAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGa > 2:404773/1‑140 (MQ=255)
aaTTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCt > 2:79927/1‑140 (MQ=255)
|
TTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCT > NC_000913/1541229‑1541486
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
