Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	100	74.7	2540932	99.2%	2520604	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	913,007	T→C	T270A (ACC→GCC)	hcp ←	protein S‑nitrosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	913,007	0	T	C	100.0%	159.6 / NA	44	T270A (ACC→GCC)	hcp	protein S‑nitrosylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/25); total (19/25)

CGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGTGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGT  >  NC_000913/912870‑913138

cGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGgcgt                                                                                                                                   >  1:1017911/1‑140 (MQ=255)

  aGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAg                                                                                                                                 >  1:95124/1‑140 (MQ=255)

  aGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAg                                                                                                                                 <  2:589578/140‑1 (MQ=255)

        tcaCGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTa                                                                                                                           >  1:827770/1‑140 (MQ=255)

        tcaCGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTa                                                                                                                           >  2:38525/1‑140 (MQ=255)

               ggggCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCg                                                                                                                    >  2:306743/1‑140 (MQ=255)

               ggggCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCg                                                                                                                    <  1:306743/140‑1 (MQ=255)

                    cAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCt                                                                                                               <  1:619480/140‑1 (MQ=255)

                      gggAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTc                                                                                                             >  2:631773/1‑140 (MQ=255)

                       ggAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCg                                                                                                            <  1:574009/140‑1 (MQ=255)

                            cGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTg                                                                                                       >  1:1089818/1‑140 (MQ=255)

                            cGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTg                                                                                                       >  1:755740/1‑140 (MQ=255)

                                 gAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGTCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCca                                                                                                  >  2:1102314/1‑140 (MQ=255)

                                 gAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCca                                                                                                  <  2:500959/140‑1 (MQ=255)

                                 gAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCca                                                                                                  <  2:95124/140‑1 (MQ=255)

                                 gAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCca                                                                                                  >  2:1073457/1‑140 (MQ=255)

                                 gAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCca                                                                                                  <  2:903675/140‑1 (MQ=255)

                                   aCTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCcagc                                                                                                <  1:396277/140‑1 (MQ=255)

                                   aCTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCcagc                                                                                                <  2:1221473/140‑1 (MQ=255)

                                   aCTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTAGGTCTGTTCcagc                                                                                                <  1:719450/140‑1 (MQ=255)

                                    cTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCcagca                                                                                               <  1:1102314/140‑1 (MQ=255)

                                       cACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGt                                                                                            >  2:1195236/1‑140 (MQ=255)

                                             cTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTaga                                                                                      >  2:1110167/1‑140 (MQ=255)

                                              tGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTagag                                                                                     <  1:631773/140‑1 (MQ=255)

                                              tGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTagag                                                                                     <  2:755740/140‑1 (MQ=255)

                                               gATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAgaga                                                                                    >  2:70267/1‑140 (MQ=255)

                                                                     gTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGaaa                                                              <  1:1000232/140‑1 (MQ=255)

                                                                       tACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATc                                                            >  2:299756/1‑140 (MQ=255)

                                                                        aCCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCa                                                           >  1:843191/1‑140 (MQ=255)

                                                                          cGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGa                                                         <  1:70267/140‑1 (MQ=255)

                                                                          cGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGa                                                         <  2:1089818/140‑1 (MQ=255)

                                                                           gACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGTAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGaa                                                        <  1:1110167/140‑1 (MQ=255)

                                                                             ccAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGaa                                                               >  2:512318/1‑131 (MQ=255)

                                                                             ccAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGaa                                                               <  1:512318/131‑1 (MQ=255)

                                                                                gATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGaa                                                        >  2:1020420/1‑135 (MQ=255)

                                                                                gATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGaa                                                        <  1:1020420/135‑1 (MQ=255)

                                                                                   gCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTa                                                <  1:1195236/140‑1 (MQ=255)

                                                                                           ttACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGt                                        <  1:299756/140‑1 (MQ=255)

                                                                                                 aGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCttt                                  >  2:640620/1‑140 (MQ=255)

                                                                                                     ccGGGGAGCCATGCGCAGGCACCATTTCGCCGTGGGCGTAAACGTTAACGCCCGTGCTTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGAAAATCAGAATGCTTTTACCCGCCGTCGCTTTGACg                              <  2:657110/140‑1 (MQ=255)

                                                                                                                  cgcAGGCAGCATTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTg                 >  2:1147128/1‑140 (MQ=255)

                                                                                                                        cagcaTTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCg           <  1:1045278/140‑1 (MQ=255)

                                                                                                                          gcaTTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCggg         <  1:334074/140‑1 (MQ=255)

                                                                                                                            aTTTCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTg       <  1:1073457/140‑1 (MQ=255)

                                                                                                                             tttCGCCGTGGGCGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGa      <  2:827770/140‑1 (MQ=255)

                                                                                                                                 gCCGTGGGCGTAGACATTAACGCCCCTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGt  >  2:226287/1‑140 (MQ=255)

CGAGGTCATCACGATGGGGCCAGGGAAACGAGCGAACTCCACTTGCTGATTCTGCCAGCCGCTGCCGTAGTTACCGACCAGATGCTTGAATTTACGCAGCTCCGGGTAGCCATGCGCAGGCAGCATTTCGCCGTGGGTGTAGACATTAACGCCCGTGCCTTCGGTCTGTTCCAGCAGGTTGTAGAGATCTTTGAGATCGTGACCGGAAATCAGAATGCATTTACCCGCCGTCGCTTTGACGTTGACCTGGGTTGGCGTCGGGTGACCGT  >  NC_000913/912870‑913138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A