Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	100	74.7	2540932	99.2%	2520604	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,108,087	C→T	W285* (TGG→TGA)	opgC ←	protein required for succinyl modification of osmoregulated periplasmic glucans

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,108,087	0	C	T	97.3%	246.1 / ‑2.9	74	W285* (TGG→TGA)	opgC	protein required for succinyl modification of osmoregulated periplasmic glucans
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (36/36); total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TAATGTATGCGCCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTA  >  NC_000913/1107951‑1108222

tAATGTATGCGCCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCAcattcaccattcac                                                                                                                                      <  1:868198/140‑1 (MQ=255)

        gcgcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCCCCATTCACAGACCGAg                                                                                                                              <  1:209692/140‑1 (MQ=255)

        gcgcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAg                                                                                                                              <  1:1022820/140‑1 (MQ=255)

        gcgcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAg                                                                                                                              <  2:403608/140‑1 (MQ=255)

        gcgcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAg                                                                                                                              >  1:158585/1‑140 (MQ=255)

          gcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGTGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATCCACAGACCGAg                                                                                                                              >  1:619193/1‑138 (MQ=255)

          gcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGTGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATCCACAGACCGAg                                                                                                                              <  2:619193/138‑1 (MQ=255)

          gcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGa                                                                                                                            >  1:618951/1‑140 (MQ=255)

          gcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGa                                                                                                                            >  2:675038/1‑140 (MQ=255)

          gcCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTGAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGa                                                                                                                            >  2:1102217/1‑140 (MQ=255)

            cGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGAcc                                                                                                                          <  1:653406/140‑1 (MQ=255)

                aaaCAGCGTTAACGGGTGGTGAACC‑‑‑‑AGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGAt                                                                                                                  <  1:287433/140‑1 (MQ=255)

                 aaCAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGt                                                                                                                     <  1:801594/140‑1 (MQ=255)

                       gTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCAc                                                                                                               <  2:158585/140‑1 (MQ=255)

                            cGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACt                                                                                                          <  1:243115/140‑1 (MQ=255)

                                tggtgAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCg                                                                                                        >  2:646524/1‑138 (MQ=255)

                                tggtgAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCg                                                                                                        <  1:646524/138‑1 (MQ=255)

                                tggtgAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGt                                                                                                      >  1:583454/1‑140 (MQ=255)

                                  gtgAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGttt                                                                                                    >  1:414147/1‑140 (MQ=255)

                                  gtgAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGttt                                                                                                    >  2:953219/1‑140 (MQ=255)

                                    gAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCg                                                                                                  <  2:414147/140‑1 (MQ=255)

                                    gAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCg                                                                                                  <  1:711825/140‑1 (MQ=255)

                                     aaCCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGt                                                                                                 >  1:973168/1‑140 (MQ=255)

                                     aaCCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGt                                                                                                 <  1:953219/140‑1 (MQ=255)

                                         agatagatAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACAt                                                                                             >  2:733362/1‑140 (MQ=255)

                                               ataAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGc                                                                                       <  1:675038/140‑1 (MQ=255)

                                                 aaaCAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGGAt                                                                                     <  1:1102217/140‑1 (MQ=255)

                                                 aaaCAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCAt                                                                                     >  1:345775/1‑140 (MQ=255)

                                                    cAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGc                                                                                  >  1:686740/1‑140 (MQ=255)

                                                     aGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCg                                                                                                  >  1:744291/1‑123 (MQ=255)

                                                     aGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCg                                                                                                  <  2:744291/123‑1 (MQ=255)

                                                     aGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGcc                                                                                 >  1:803239/1‑140 (MQ=255)

                                                     aGCGATGCGTTAACAAAATAAGTAACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGcc                                                                                 >  2:222144/1‑140 (MQ=255)

                                                      gCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCa                                                                                <  1:1083536/140‑1 (MQ=255)

                                                      gCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCa                                                                                <  1:318458/140‑1 (MQ=255)

                                                         aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATc                                                                                            >  2:955533/1‑125 (MQ=255)

                                                         aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATc                                                                                            <  1:955533/125‑1 (MQ=255)

                                                         aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTg                                                                             >  2:957213/1‑140 (MQ=255)

                                                         aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTg                                                                             >  1:413719/1‑140 (MQ=255)

                                                         aTGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTg                                                                             >  2:1147812/1‑140 (MQ=255)

                                                            cGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTGACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCa                                                                          >  2:705187/1‑140 (MQ=255)

                                                            cGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCa                                                                          >  2:367186/1‑140 (MQ=255)

                                                                aaCAAAATAAGTAACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAgc                                                                      <  1:222144/140‑1 (MQ=255)

                                                                      aTAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGt                                                                >  1:1230105/1‑140 (MQ=255)

                                                                       tAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGTCCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCAt                                                                         <  1:510833/130‑1 (MQ=255)

                                                                       tAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGGCCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCAt                                                                         >  2:510833/1‑130 (MQ=255)

                                                                       tAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGtt                                                               <  1:957213/140‑1 (MQ=255)

                                                                       tAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGtt                                                               <  1:733362/140‑1 (MQ=255)

                                                                             aCCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTaa                                                         >  2:1011945/1‑140 (MQ=255)

                                                                                 gcgcTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATaa                                                     >  1:476848/1‑140 (MQ=255)

                                                                                  cgcTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAg                                                    >  1:494430/1‑140 (MQ=255)

                                                                                  cgcTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAg                                                    <  2:583454/140‑1 (MQ=255)

                                                                                       aCTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACa                                               <  2:413719/140‑1 (MQ=255)

                                                                                               ttAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCaa                                       <  2:913045/140‑1 (MQ=255)

                                                                                                tAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTCGAGTAAATAAGCGACAAACGCCAAt                                      <  1:1190593/140‑1 (MQ=255)

                                                                                                 aaGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAAtg                                     >  1:540516/1‑140 (MQ=255)

                                                                                                    cAAACGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAAtgctg                                  <  2:476848/140‑1 (MQ=255)

                                                                                                      aaCGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAAtgctgct                                >  1:100857/1‑140 (MQ=255)

                                                                                                      aaCGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAAtgctgct                                >  2:1229719/1‑140 (MQ=255)

                                                                                                        cGGTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAAtgctgctgc                              <  2:494430/140‑1 (MQ=255)

                                                                                                         ggTGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGGTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCa                             >  1:970248/1‑140 (MQ=255)

                                                                                                           tGGCCAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAg                           >  1:1191885/1‑140 (MQ=255)

                                                                                                              ccAAAGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGt                        <  2:381505/140‑1 (MQ=255)

                                                                                                                aaaGGAGAAGACCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTAc                      <  1:114409/140‑1 (MQ=255)

                                                                                                                       aagaCCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCa                             <  1:149463/126‑1 (MQ=255)

                                                                                                                       aagaCCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCa                             >  2:149463/1‑126 (MQ=255)

                                                                                                                          aCCACATTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACgaga            <  2:100857/140‑1 (MQ=255)

                                                                                                                            cacaTTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACg          >  1:1176750/1‑140 (MQ=255)

                                                                                                                            cacaTTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACg          <  2:540516/140‑1 (MQ=255)

                                                                                                                             acaTTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACgg         <  1:1147812/140‑1 (MQ=255)

                                                                                                                               aTTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCg       >  1:1026906/1‑140 (MQ=255)

                                                                                                                               aTTCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTAAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCg       >  1:434290/1‑140 (MQ=255)

                                                                                                                                ttCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGt      <  1:705187/140‑1 (MQ=255)

                                                                                                                                ttCACCATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGt      <  1:367186/140‑1 (MQ=255)

                                                                                                                                    ccATTCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTa  >  2:1106139/1‑140 (MQ=255)

TAATGTATGCGCCGAAAAACAGCGTTAACGGGTGGTGAACCAGATAGATAAACAGCGATGCGTTAACAAAATAAGTCACCCGCGCTGACTGGAAGTTAAGCAAACGGTGGCCAAAGGAGAAGACCACATTCACCATCCACAGACCGAGGACCATGGTGATCACCGACTCGGTTTCGTACATCCAGGCATCGCCACTGCCATAGCGCTGGTTGAGTAAATAAGCGACAAACGCCAATGCTGCTGCAAGGGTACAGCCACGAGACGGCGTGGTA  >  NC_000913/1107951‑1108222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A