Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F28 I1 R1	139	53.6	1844736	98.1%	1809686	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,109,534	A→G	D67G (GAC→GGC)	opgG →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,109,534	0	A	G	100.0%	181.1 / NA	50	D67G (GAC→GGC)	opgG	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/28); total (22/28)

GGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGAT  >  NC_000913/1109397‑1109667

ggCTTTCAGTATTGATGATGTCACAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCa                                                                                                                                     >  1:886286/1‑140 (MQ=255)

 gCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCaa                                                                                                                                    >  2:289004/1‑140 (MQ=255)

   tttCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAg                                                                                                                                  <  2:571394/140‑1 (MQ=255)

            tgatgaTGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGa                                                                                                                         <  1:450749/140‑1 (MQ=255)

             gatgatGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGaa                                                                                                                        >  1:195835/1‑140 (MQ=255)

              atgatgTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAAc                                                                                                                       <  1:345760/140‑1 (MQ=255)

              atgatgTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAAc                                                                                                                       <  1:439050/140‑1 (MQ=255)

              atgatgTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAAc                                                                                                                       <  2:688671/140‑1 (MQ=255)

                   gTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCt                                                                                                                  >  1:655648/1‑140 (MQ=255)

                     cGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGa                                                                                                                <  2:700619/140‑1 (MQ=255)

                     cGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGa                                                                                                                <  2:578915/140‑1 (MQ=255)

                          agcaagcTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGAcc                                                                                                           <  2:334443/140‑1 (MQ=255)

                          agcaagcTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGAcc                                                                                                           <  2:886286/140‑1 (MQ=255)

                            caagcTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGATGAcccc                                                                                                         >  1:313929/1‑140 (MQ=255)

                                     tCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACt                                                                                                <  2:857309/140‑1 (MQ=255)

                                            ccGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTc                                                                                         <  2:195835/140‑1 (MQ=255)

                                            ccGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTc                                                                                         <  2:533387/140‑1 (MQ=255)

                                               ggAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTAc                                                                                      >  2:897860/1‑140 (MQ=255)

                                                   aGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATc                                                                                  <  2:370682/140‑1 (MQ=255)

                                                   aGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATc                                                                                  <  2:90926/140‑1 (MQ=255)

                                                   aGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATc                                                                                  <  2:256853/140‑1 (MQ=255)

                                                           agaCGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGATGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATg                                                                          <  2:313929/140‑1 (MQ=255)

                                                               gCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATg                                                                          >  2:144008/1‑136 (MQ=255)

                                                               gCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATg                                                                          <  1:144008/136‑1 (MQ=255)

                                                               gCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACt                                                                      >  1:773975/1‑140 (MQ=255)

                                                                cccAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACtt                                                                     <  2:773975/140‑1 (MQ=255)

                                                                   aaaaGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGa                                                                  >  2:822447/1‑140 (MQ=255)

                                                                           cTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCgg                                                          >  2:181427/1‑140 (MQ=255)

                                                                           cTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCgg                                                          >  1:448714/1‑140 (MQ=255)

                                                                              gCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATA‑CCCGGTCaa                                                      <  1:508446/140‑1 (MQ=255)

                                                                               cccTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGCACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCaa                                                      <  2:834321/140‑1 (MQ=255)

                                                                               cccTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCaa                                                      <  2:673762/140‑1 (MQ=255)

                                                                                   ccGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAaaata                                                  >  2:870652/1‑140 (MQ=255)

                                                                                                          gcgGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                           >  1:169241/1‑140 (MQ=255)

                                                                                                          gcgGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                           >  1:394246/1‑140 (MQ=255)

                                                                                                          gcgGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                           >  2:80051/1‑140 (MQ=255)

                                                                                                          gcgGACTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGt                           <  1:897860/140‑1 (MQ=255)

                                                                                                              aCTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCaaa                       >  2:548851/1‑140 (MQ=255)

                                                                                                               cTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAAc                      <  1:80051/140‑1 (MQ=255)

                                                                                                               cTATCAGCAGATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAAc                      <  1:289004/140‑1 (MQ=255)

                                                                                                                    agcagATCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATc                 >  1:417218/1‑140 (MQ=255)

                                                                                                                         aTCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATa            <  1:822447/140‑1 (MQ=255)

                                                                                                                          tCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATAc           >  2:65155/1‑140 (MQ=255)

                                                                                                                          tCCAGTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATAc           >  2:425985/1‑140 (MQ=255)

                                                                                                                              gTTTAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGcc       >  2:380001/1‑140 (MQ=255)

                                                                                                                                ttAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCg     >  2:405513/1‑140 (MQ=255)

                                                                                                                                ttAATCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCg     >  1:94598/1‑140 (MQ=255)

                                                                                                                                  aaTCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGa   <  1:819134/140‑1 (MQ=255)

                                                                                                                                  aaTCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGCTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACAAATCAAATACAGCCCGGa   <  2:655648/140‑1 (MQ=255)

                                                                                                                                   aTCATGGCAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGat  <  2:394246/140‑1 (MQ=255)

GGCTTTCAGTATTGATGATGTCGCAAAGCAAGCTCAATCTTTAGCCGGGAAAGGCTACGAGACGCCCAAAAGCAACTTGCCCTCCGTTTTCCGCGATATGAAATACGCGGACTATCAGCAGATCCAGTTTAATCATGACAAAGCGTACTGGAACAATCTGAAGACCCCATTCAAACTCGAGTTCTACCATCAGGGTATGTACTTCGATACCCCGGTCAAAATAAATGAAGTGACTGCCACCGCAGTCAAACGAATCAAATACAGCCCGGAT  >  NC_000913/1109397‑1109667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A