Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	161	50.8	1735388	98.6%	1711092	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,110,359	A→G	E342G (GAG→GGG)	opgG →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,110,359	0	A	G	100.0%	131.1 / NA	38	E342G (GAG→GGG)	opgG	osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/15); total (23/15)

CGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGAGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCACCTT  >  NC_000913/1110227‑1110494

cGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGggggtggggt                                                                                                                                  <  2:837366/140‑1 (MQ=255)

     agcagcTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAgg                                                                                                                             <  2:841767/140‑1 (MQ=255)

                    gAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCtggg                                                                                                              >  2:795298/1‑139 (MQ=255)

                        aCCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAGGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGaa                                                                                                          >  2:617116/1‑140 (MQ=255)

                             cAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTcc                                                                                                     <  1:442596/140‑1 (MQ=255)

                                  cTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTccaacca                                                                                                <  2:362598/140‑1 (MQ=255)

                                   tttGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTTACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTccaaccaa                                                                                               >  1:274812/1‑140 (MQ=255)

                                     tGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACg                                                                                             >  1:405174/1‑140 (MQ=255)

                                            ttGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAc                                                                                      >  1:801666/1‑140 (MQ=255)

                                            ttGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAc                                                                                      >  1:33264/1‑140 (MQ=255)

                                             tGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAcc                                                                                     <  1:119965/140‑1 (MQ=255)

                                                  cgcgGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGa                                                                                >  2:40610/1‑140 (MQ=255)

                                                  cgcgGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGa                                                                                >  1:829460/1‑140 (MQ=255)

                                                    cgGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGTGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATa                                                                              >  2:803920/1‑140 (MQ=255)

                                                      gTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAc                                                                                      >  1:286367/1‑130 (MQ=255)

                                                      gTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAAc                                                                                      <  2:286367/130‑1 (MQ=255)

                                                                             gATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGa                                                     <  1:617116/140‑1 (MQ=255)

                                                                                ctcGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCa                                                  >  1:348105/1‑140 (MQ=255)

                                                                                   gatgatCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCt                                               >  2:132012/1‑140 (MQ=255)

                                                                                        tCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCgg                                          >  1:252352/1‑140 (MQ=255)

                                                                                        tCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCgg                                          >  1:247001/1‑140 (MQ=255)

                                                                                          gTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAg                                        <  1:132012/140‑1 (MQ=255)

                                                                                           ttACGATCTCCGTCCAAGCGCATGGGTGACTCCGAACGGGGGGAGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGc                                       >  2:781405/1‑140 (MQ=255)

                                                                                                 tctcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTa                                 >  1:206687/1‑140 (MQ=255)

                                                                                                   tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCTACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGc                                       <  2:773692/132‑1 (MQ=255)

                                                                                                   tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGc                                       >  1:773692/1‑132 (MQ=255)

                                                                                                   tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTaaa                               >  1:530882/1‑140 (MQ=255)

                                                                                                   tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTaaa                               >  2:752568/1‑140 (MQ=255)

                                                                                                   tcCGTCCAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTaaa                               <  1:547440/140‑1 (MQ=255)

                                                                                                        ccAAGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGAt                          <  2:33264/140‑1 (MQ=255)

                                                                                                           aGCGCATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGaa                       >  2:608573/1‑140 (MQ=255)

                                                                                                            gcgcATGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAAc                      <  2:348105/140‑1 (MQ=255)

                                                                                                                 tGGGTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTaa                 >  1:354970/1‑140 (MQ=255)

                                                                                                                    gTGACTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATa              <  1:839925/140‑1 (MQ=255)

                                                                                                                      taCTCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTGAAGAGATGAACTTTAAATaca            <  2:274812/139‑1 (MQ=255)

                                                                                                                         tCCGAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCa         <  2:354970/140‑1 (MQ=255)

                                                                                                                            gAAAGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCa      <  2:829460/140‑1 (MQ=255)

                                                                                                                             aaaGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCAc     >  2:254600/1‑140 (MQ=255)

                                                                                                                              aaGGGGGGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCAcc    >  1:354440/1‑140 (MQ=255)

                                                                                                                                ggggggTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCACCtt  <  2:801666/140‑1 (MQ=255)

                                                                                                                                ggggggTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCACCtt  <  1:254600/140‑1 (MQ=255)

CGGTCAGCAGCTTCTCCATGGAAAACCCGCAAGGCTTTGGTCTGTTGCAGCGCGGTCGTGATTTCTCCCGCTTTGAAGATCTCGATGATCGTTACGATCTCCGTCCAAGCGCATGGGTGACTCCGAAAGGGGAGTGGGGTAAAGGCAGCGTTGAGCTGGTGGAAATTCCAACCAACGATGAAACCAACGATAACATCGTCGCTTACTGGACGCCGGATCAGCTGCCGGAGCCGGGTAAAGAGATGAACTTTAAATACACCATCACCTT  >  NC_000913/1110227‑1110494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A