Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	100	74.7	2540932	99.2%	2520604	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,257,285	A→G	G176G (GGT→GGC)	ychF ←	redox‑responsive ATPase YchF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,257,285	0	A	G	100.0%	186.1 / NA	50	G176G (GGT→GGC)	ychF	redox‑responsive ATPase YchF
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (33/17); total (33/17)

GGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCAGCG  >  NC_000913/1257163‑1257415

gggTTGTTTTCAAAACCGTTTTCGTTGACGTTGGCGATGTAAATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:1031315/1‑140 (MQ=255)

gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:987462/1‑140 (MQ=255)

gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:982279/1‑140 (MQ=255)

gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:847345/1‑140 (MQ=255)

gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:205911/1‑140 (MQ=255)

gggTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTg                                                                                                                   >  2:1079483/1‑140 (MQ=255)

     gttTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGggca                                                                                                              >  1:806408/1‑140 (MQ=255)

     gttTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGggca                                                                                                              >  1:186592/1‑140 (MQ=255)

      ttttCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGggcag                                                                                                             >  2:618075/1‑140 (MQ=255)

          cAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAt                                                                                                         <  2:806408/140‑1 (MQ=255)

          cAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAt                                                                                                         <  1:496727/140‑1 (MQ=255)

             aaCCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAtttt                                                                                                      >  1:673868/1‑140 (MQ=255)

                  tCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAt                                                                                                         >  1:426257/1‑132 (MQ=255)

                  tCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCAt                                                                                                         <  2:426257/132‑1 (MQ=255)

                   cTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAgg                                                                                                <  1:1262672/140‑1 (MQ=255)

                   cTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAgg                                                                                                <  2:1230176/140‑1 (MQ=255)

                   cTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAgg                                                                                                >  2:822389/1‑140 (MQ=255)

                           aCGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCcag                                                                                        <  2:186592/140‑1 (MQ=255)

                                 gCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCcagctcagc                                                                                  >  1:426661/1‑140 (MQ=255)

                                  cGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCcagctcagct                                                                                 >  2:616646/1‑140 (MQ=255)

                                            tgttgGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCtt                                                                       >  2:453316/1‑140 (MQ=255)

                                               tgGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTAt                                                                    >  1:335168/1‑140 (MQ=255)

                                                ggTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAACTCAGCTTTCGCGTCTTTATc                                                                   >  1:1013259/1‑140 (MQ=255)

                                                 gTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTTTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCg                                                                  >  2:1095049/1‑140 (MQ=255)

                                                 gTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCg                                                                  >  1:339102/1‑140 (MQ=255)

                                                   ttCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGcc                                                                >  2:934963/1‑140 (MQ=255)

                                                        cGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCtt                                                           <  2:673868/140‑1 (MQ=255)

                                                        cGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCtt                                                           <  2:335168/140‑1 (MQ=255)

                                                          tCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTg                                                         >  1:1024460/1‑140 (MQ=255)

                                                           cAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTgg                                                        >  1:425838/1‑140 (MQ=255)

                                                            aGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGc                                                       >  1:421191/1‑140 (MQ=255)

                                                             ggAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCt                                                      <  2:1024460/140‑1 (MQ=255)

                                                                  cTCAGGTAAAGAATAACCGCTTTCTCTTCAGGGCTTAAATCCAGCGCGCGCAGGATGCCTGTGTTTTCCAAGTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTttctt                                                 <  2:243547/140‑1 (MQ=255)

                                                                        tAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTAc                                           >  1:787476/1‑140 (MQ=255)

                                                                          aCGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTAcgc                                         >  2:295569/1‑140 (MQ=255)

                                                                            gAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGa                                       >  2:853182/1‑140 (MQ=255)

                                                                               tAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGa                                    >  1:296466/1‑140 (MQ=255)

                                                                                      tttCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAAt                                  <  2:970948/135‑1 (MQ=255)

                                                                                      tttCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAAt                                  >  1:970948/1‑135 (MQ=255)

                                                                                       ttCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACg                            <  1:1131754/140‑1 (MQ=255)

                                                                                               aGCGCTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAgg                    >  2:1162985/1‑140 (MQ=255)

                                                                                                 cgcTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGgtg                  >  2:322935/1‑140 (MQ=255)

                                                                                                  gcTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGgtgt                 <  1:1162985/140‑1 (MQ=255)

                                                                                                  gcTTAAATCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGgtgt                 <  1:1207001/140‑1 (MQ=255)

                                                                                                        aTCCAGCGCGCGCAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGt           <  1:205911/140‑1 (MQ=255)

                                                                                                             gcgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGcc      <  1:1095049/140‑1 (MQ=255)

                                                                                                             gcgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGcc      <  1:618075/140‑1 (MQ=255)

                                                                                                              cgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTTGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCa     >  1:505371/1‑140 (MQ=255)

                                                                                                              cgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGTGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCa     >  2:1260098/1‑140 (MQ=255)

                                                                                                              cgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCa     >  1:23397/1‑140 (MQ=255)

                                                                                                              cgcgcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCACCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCa     >  2:180470/1‑140 (MQ=255)

                                                                                                                 gcgcAGCATGCCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGTGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCAgcg  <  1:1260098/140‑1 (MQ=255)

GGGTTGTTTTCAAAACCGTCTTCGTTGACGTTGGCGATGTACATTGTTGGTTTCAGCGTCAGGAAGCTCAGGTAACGAATAGCCGCTTTCTCTTCAGCGCTTAAATCCAGCGCGCGCAGCATACCTGCATTTTCCAACTGGGGCAGGCATTTTTCCAGGACCGCCAGCTCAGCTTTCGCGTCTTTATCGCCACCTTTGGCTTTCTTCTGTACGCGATGAATCGCACGTTCGCAGGTGTCGAGGTCTGCCAGCG  >  NC_000913/1257163‑1257415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A