Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 100 50.0 1731240 97.4% 1686227 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,296,446 T→C Y559C (TAC→TGC)  adhE ← aldehyde‑alcohol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,296,4460TC100.0% 171.9 / NA 48Y559C (TAC→TGC) adhEaldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

TTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAGGGTCGGG  >  NC_000913/1296310‑1296565
                                                                                                                                        |                                                                                                                       
ttCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGcaca                                                                                                                      <  1:622795/140‑1 (MQ=255)
ttCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGcaca                                                                                                                      <  2:280496/140‑1 (MQ=255)
 tCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACAt                                                                                                                     <  2:774899/140‑1 (MQ=255)
   aGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATaa                                                                                                                   <  1:243136/140‑1 (MQ=255)
      aCCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAAccc                                                                                                                >  1:234479/1‑140 (MQ=255)
          gTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACAt                                                                                                            >  2:399490/1‑140 (MQ=255)
            aCCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGa                                                                                                          >  2:167958/1‑140 (MQ=255)
                    tggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTcgcg                                                                                                  <  1:350986/140‑1 (MQ=255)
                     ggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCgcgg                                                                                                 >  1:108020/1‑140 (MQ=255)
                     ggtggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCgcgg                                                                                                 >  2:838091/1‑140 (MQ=255)
                       tggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCgcggcg                                                                                               <  2:108020/140‑1 (MQ=255)
                       tggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCgcggcg                                                                                               <  2:234479/140‑1 (MQ=255)
                        ggtgACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGt                                                                                              >  1:190317/1‑140 (MQ=255)
                           gACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCa                                                                                           >  2:757789/1‑140 (MQ=255)
                            aCAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTcgcg                                                                                                  <  2:263007/132‑1 (MQ=255)
                            aCAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTcgcg                                                                                                  >  1:263007/1‑132 (MQ=255)
                            aCAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCAt                                                                                          <  1:838091/140‑1 (MQ=255)
                                cGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCggg                                                                                      >  2:803797/1‑140 (MQ=255)
                                 gATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCgggg                                                                                     <  1:399490/140‑1 (MQ=255)
                                               tCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAgc                                                                       >  1:664272/1‑140 (MQ=255)
                                                cACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCgggg                                                                                     >  2:713842/1‑125 (MQ=255)
                                                cACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCgggg                                                                                     <  1:713842/125‑1 (MQ=255)
                                                             gggAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTc                                                         <  2:664272/140‑1 (MQ=255)
                                                                     gTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGa                                                 <  1:757789/140‑1 (MQ=255)
                                                                       aGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAg                                               <  1:803797/140‑1 (MQ=255)
                                                                          tACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAg                                            <  2:190317/140‑1 (MQ=255)
                                                                          tACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAg                                            <  1:391411/140‑1 (MQ=255)
                                                                               ttACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGc                                       >  2:316066/1‑140 (MQ=255)
                                                                                       atCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTg                               >  1:721237/1‑140 (MQ=255)
                                                                                       atCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTg                               >  1:541301/1‑140 (MQ=255)
                                                                                       atCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTg                               <  1:167958/140‑1 (MQ=255)
                                                                                        tCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGc                              >  1:235300/1‑140 (MQ=255)
                                                                                         ccATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCa                             <  1:316066/140‑1 (MQ=255)
                                                                                            tAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCt                          <  1:755374/140‑1 (MQ=255)
                                                                                              aaGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCttt                        >  2:497834/1‑140 (MQ=255)
                                                                                               agcgcagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTa                       >  1:762750/1‑140 (MQ=255)
                                                                                                 cgcagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacg                     >  1:119143/1‑140 (MQ=255)
                                                                                                 cgcagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacg                     >  2:57776/1‑140 (MQ=255)
                                                                                                 cgcagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacg                     >  1:482371/1‑140 (MQ=255)
                                                                                                   cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   >  2:41862/1‑140 (MQ=255)
                                                                                                   cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   <  2:721237/140‑1 (MQ=255)
                                                                                                   cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   <  2:762750/140‑1 (MQ=255)
                                                                                                   cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   >  2:774688/1‑140 (MQ=255)
                                                                                                   cagcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaa                   >  2:838492/1‑140 (MQ=255)
                                                                                                    agcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaac                  >  1:522469/1‑140 (MQ=255)
                                                                                                     gcgcCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTacgaacg                 <  2:746033/140‑1 (MQ=255)
                                                                                                          aGCTCTTCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCt            <  2:482371/140‑1 (MQ=255)
                                                                                                               ttCGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAggg       <  1:57776/140‑1 (MQ=255)
                                                                                                                 cGAAGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAgggtc     <  2:235300/140‑1 (MQ=255)
                                                                                                                    aGTGAGTTTCCGGATGTTCGCACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAgggtcggg  <  1:632174/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                       
TTCAGAACCTGTACCAGAAGTGGTGGTGACAGCGATCATTTTCGCTTTCACGCCCATTTTCGGGAACTTGTAGATACGTTTACGGATATCCATAAAGCGCAGCGCCAGCTCTTCGAAGTGAGTTTCCGGATGTTCGTACATAACCCACATGATCTTCGCGGCGTCCATCGGGGAACCACCACCCAGCGCGATAATCACGTCTGGTTTGAAGGAGTTTGCCAGTTCTGCACCTTTACGAACGATGCTCAGGGTCGGG  >  NC_000913/1296310‑1296565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A