Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	157	47.3	1607120	99.4%	1597477	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,128,342	C→T	N168N (AAC→AAT)	murJ →	putative lipid II flippase MurJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,128,342	0	C	T	100.0%	128.0 / NA	37	N168N (AAC→AAT)	murJ	putative lipid II flippase MurJ
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/20); total (17/20)

ATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCAT  >  NC_000913/1128207‑1128476

aTTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCa                                                                                                                                    <  1:718155/140‑1 (MQ=255)

       cTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGAt                                                                                                                             <  1:295150/140‑1 (MQ=255)

                 agcTACTAAAGATTACCTTTCCCTATATCTTGTTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGGTCCAACACTGCTTAATATCAGCATGATTGGTTTcgcg                                                                                                                   <  2:709543/140‑1 (MQ=255)

                    tactaAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTg                                                                                                                >  2:315913/1‑140 (MQ=255)

                             ttACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCa                                                                                                       >  1:173432/1‑140 (MQ=255)

                              tACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAc                                                                                                      <  2:762587/140‑1 (MQ=255)

                               aCCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCAcc                                                                                                     >  1:97841/1‑140 (MQ=255)

                                ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                                                                                    >  2:558278/1‑140 (MQ=255)

                                ccTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCg                                                                                                    >  1:206631/1‑140 (MQ=255)

                                     cccTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACtt                                                                                               >  1:217623/1‑140 (MQ=255)

                                             cTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCAc                                                                                       <  2:58865/140‑1 (MQ=255)

                                             cTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCAc                                                                                       <  1:315913/140‑1 (MQ=255)

                                               tGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCg                                                                                     >  1:483696/1‑140 (MQ=255)

                                               tGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCg                                                                                     >  2:319876/1‑140 (MQ=255)

                                                 cTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCAc                                                                                       >  1:324273/1‑136 (MQ=255)

                                                 cTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCAc                                                                                       <  2:324273/136‑1 (MQ=255)

                                                   gATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgc                                                                                 <  1:558278/140‑1 (MQ=255)

                                                        cccTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcg                                                                            >  1:799327/1‑140 (MQ=255)

                                                             gCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTgctggcgctggc                                                                       <  1:350312/140‑1 (MQ=255)

                                                              cGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGctggcgctggcg                                                                      <  1:287440/140‑1 (MQ=255)

                                                                  gCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTggg                                                                  <  2:217623/140‑1 (MQ=255)

                                                                   cTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGc                                                                 >  1:194269/1‑140 (MQ=255)

                                                                   cTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGc                                                                 >  1:225306/1‑140 (MQ=255)

                                                                                ttCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTcggc                                                    >  1:199562/1‑140 (MQ=255)

                                                                                          gTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGc                                          >  1:208969/1‑140 (MQ=255)

                                                                                               aCCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTg                                     <  2:206631/140‑1 (MQ=255)

                                                                                                      ctcGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATc                              <  1:121180/140‑1 (MQ=255)

                                                                                                       tcGATTCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCa                             <  2:225306/140‑1 (MQ=255)

                                                                                                            tCCGGCGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTAc                        <  2:85092/140‑1 (MQ=255)

                                                                                                              cGGCGTTTGCTCCAACAGTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCg                      >  1:603556/1‑140 (MQ=255)

                                                                                                                 cGTTTGCTCCAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCAc                   <  2:30649/140‑1 (MQ=255)

                                                                                                                      gCTCCAACAGTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTgaa              <  2:603556/140‑1 (MQ=255)

                                                                                                                         ccAACACTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTgaagaa           <  2:173432/140‑1 (MQ=255)

                                                                                                                             cacTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATc       >  1:717048/1‑140 (MQ=255)

                                                                                                                              acTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCg      <  2:527440/140‑1 (MQ=255)

                                                                                                                               cTGCTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCgg     <  2:97841/140‑1 (MQ=255)

                                                                                                                                  cTTAATATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCAt  >  1:674635/1‑140 (MQ=255)

ATTTGCCCTGACCAGCCAGCTACTAAAGATTACCTTTCCCTATATCTTGCTGATCTCCCTGGCGTCGCTGGTGGGAGCGATTCTGAATACGTGGAACCGCTTCTCGATTCCGGCGTTTGCTCCAACACTGCTTAACATCAGCATGATTGGTTTCGCGCTGTTTGCCGCACCGTACTTTAACCCACCGGTGCTGGCGCTGGCGTGGGCTGTTACGGTCGGCGGCGTCCTGCAGCTGGTGTATCAGCTACCGCACCTGAAGAAGATCGGCAT  >  NC_000913/1128207‑1128476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A