Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F28 I1 R1	157	47.3	1607120	99.4%	1597477	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,248,684	A→G	I144T (ATC→ACC)	dhaM ←	dihydroxyacetone kinase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,248,684	0	A	G	100.0%	132.4 / NA	38	I144T (ATC→ACC)	dhaM	dihydroxyacetone kinase subunit M
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/19); total (19/19)

CAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGA  >  NC_000913/1248548‑1248813

cAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGCGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGttt                                                                                                                                <  2:715384/140‑1 (MQ=255)

      cATATCGACATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAgtgt                                                                                                                          <  2:295965/140‑1 (MQ=255)

           cGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCggag                                                                                                                     <  2:690857/140‑1 (MQ=255)

           cGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCggag                                                                                                                     >  2:587303/1‑140 (MQ=255)

           cGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCggag                                                                                                                     <  1:587303/140‑1 (MQ=255)

                ttAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACgg                                                                                                                >  2:641558/1‑140 (MQ=255)

                 tAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGt                                                                                                               <  1:641558/140‑1 (MQ=255)

                       tCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAAccc                                                                                                         >  2:511911/1‑140 (MQ=255)

                         gATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAg                                                                                                       <  1:511911/140‑1 (MQ=255)

                                 ataAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCAc                                                                                               <  1:190499/140‑1 (MQ=255)

                                          ccGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCtt                                                                                      >  1:111083/1‑140 (MQ=255)

                                          ccGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCtt                                                                                      <  1:280059/140‑1 (MQ=255)

                                          ccGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCtt                                                                                      <  1:442221/140‑1 (MQ=255)

                                            gggAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTcc                                                                                    >  2:98036/1‑140 (MQ=255)

                                                            aTGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGt                                                                    <  1:420616/140‑1 (MQ=255)

                                                                  gCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGt                                                                    >  1:219353/1‑134 (MQ=255)

                                                                  gCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGt                                                                    <  2:219353/134‑1 (MQ=255)

                                                                  gCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGa                                                              >  2:437977/1‑140 (MQ=255)

                                                                  gCCGTTACGGTTTTTTATGACCACCGCAAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGa                                                              >  2:653216/1‑140 (MQ=255)

                                                                       tACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt                                                         >  1:347694/1‑140 (MQ=255)

                                                                                   tGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATcc                                             <  2:111083/140‑1 (MQ=255)

                                                                                   tGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATcc                                             >  1:661049/1‑140 (MQ=255)

                                                                                         ccGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccccc                                       >  1:93890/1‑140 (MQ=255)

                                                                                          cGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCg                                      >  2:124449/1‑140 (MQ=255)

                                                                                                    cGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCt                            >  1:393426/1‑140 (MQ=255)

                                                                                                          tcttcATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGt                      >  1:323570/1‑140 (MQ=255)

                                                                                                          tcttcATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGt                      >  1:482814/1‑140 (MQ=255)

                                                                                                              cATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAcc                        <  2:352447/134‑1 (MQ=255)

                                                                                                              cATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAcc                        >  1:352447/1‑134 (MQ=255)

                                                                                                              cATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgtt                  <  2:482814/140‑3 (MQ=255)

                                                                                                              cATCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgct                  <  1:98036/140‑1 (MQ=255)

                                                                                                                tCGTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgctgc                <  1:763635/140‑1 (MQ=255)

                                                                                                                  gTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTTACCGTtgctgc                >  2:632965/1‑138 (MQ=255)

                                                                                                                  gTACGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTTACCGTtgctgc                <  1:632965/138‑1 (MQ=255)

                                                                                                                     cGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGa                          <  2:588706/125‑1 (MQ=255)

                                                                                                                     cGCAGGACATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGa                          >  1:588706/1‑125 (MQ=255)

                                                                                                                           aCATGTGTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCtt     <  2:661049/140‑1 (MQ=255)

                                                                                                                              tgtgTCAGAGGTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGa  >  2:372016/1‑140 (MQ=255)

CAGCAACATATCGGCATTAAATGTCGATAAGGTATAAACCAGCCGGGAGGCCGGACGTACATGCAGGCCGTTACGGTTTTTTATGACCACCGCCAGAGAACGGGCTTCTTCATCGTACGCAGGACATGTGTCAGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGA  >  NC_000913/1248548‑1248813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A