Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
118 |
32.6 |
1094092 |
99.3% |
1086433 |
139.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
945,505 |
A→G |
V18A (GTT→GCT) |
ycaC ← |
putative hydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 945,505 | 0 | A | G | 100.0%
| 89.4
/ NA
| 26 | V18A (GTT→GCT) | ycaC | putative hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/11); total (15/11) |
TTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACGGGAAATA > NC_000913/945374‑945639
|
tttCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCaaa > 1:420098/1‑140 (MQ=255)
tGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGAc < 1:396897/140‑1 (MQ=255)
ggTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACg > 2:12119/1‑140 (MQ=255)
tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTc < 1:12119/140‑1 (MQ=255)
tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTc > 1:202786/1‑140 (MQ=255)
tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTc > 1:63954/1‑140 (MQ=255)
gTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCctct > 2:445190/1‑140 (MQ=255)
gTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCctct > 2:149930/1‑140 (MQ=255)
tCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCtctt < 2:202786/140‑1 (MQ=255)
aCCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCtcttct < 2:305576/140‑1 (MQ=255)
cgcCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGAGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTc > 2:317529/1‑140 (MQ=255)
gcCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCg > 1:91072/1‑140 (MQ=255)
gCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGagag < 1:149930/140‑1 (MQ=255)
ttATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGt > 2:236237/1‑140 (MQ=255)
tATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGaaa > 1:451197/1‑140 (MQ=255)
aTCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAAt > 1:346017/1‑140 (MQ=255)
gggTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGa < 1:534303/140‑1 (MQ=255)
gggAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGctc > 2:356567/1‑140 (MQ=255)
gAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTg < 2:346017/140‑1 (MQ=255)
aaaCCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCa < 1:236237/140‑1 (MQ=255)
ccAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAg > 2:70034/1‑140 (MQ=255)
ccAGCCTGGAGATCAGCAAGCAAAACGGCAGTATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAg < 1:317529/140‑1 (MQ=255)
ccTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACgg < 2:451197/140‑1 (MQ=255)
ccTGGTGATCAGCAAGCAAAACGGCAGCATCA‑TTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACggg > 1:110703/1‑140 (MQ=255)
cTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACggg > 1:321146/1‑140 (MQ=255)
tGATCAGCAAGCAAAACGGCAGCATCA‑TTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACGGGAAata < 2:110703/140‑1 (MQ=255)
|
TTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACGGGAAATA > NC_000913/945374‑945639
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
