Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	118	32.6	1094092	99.3%	1086433	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,023,670	A→G	F72L (TTC→CTC)	yccS ←	putative transporter YccS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,023,670	0	A	G	100.0%	96.7 / NA	31	F72L (TTC→CTC)	yccS	putative transporter YccS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/18); total (13/18)

AAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTG  >  NC_000913/1023534‑1023798

aaGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAagcagagcag                                                                                                                               >  2:35552/1‑140 (MQ=255)

   gCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGt                                                                                                                            >  1:416204/1‑140 (MQ=255)

   gCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGt                                                                                                                            >  1:454927/1‑140 (MQ=255)

         gttgCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAatgat                                                                                                                      <  2:426686/140‑1 (MQ=255)

                    ttGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCa                                                                                                           >  1:216624/1‑140 (MQ=255)

                    ttGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCa                                                                                                           <  2:401936/140‑1 (MQ=255)

                    ttGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCa                                                                                                           >  1:114585/1‑140 (MQ=255)

                            gACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTccc                                                                                                   >  1:63861/1‑140 (MQ=255)

                                gccgAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCa                                                                                               <  1:53604/140‑1 (MQ=255)

                                 ccgAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAg                                                                                              >  1:65751/1‑140 (MQ=255)

                                    aGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCg                                                                                           <  1:241128/140‑1 (MQ=255)

                                         aaTGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCAt                                                                                      <  2:445518/140‑1 (MQ=255)

                                                   cTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCg                                                                                           >  1:72262/1‑125 (MQ=255)

                                                   cTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCg                                                                                           <  2:72262/125‑1 (MQ=255)

                                                   cTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGt                                                                            >  1:317548/1‑140 (MQ=255)

                                                        agagagCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAgcg                                                                       <  1:35552/140‑1 (MQ=255)

                                                         gagagCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAgcgc                                                                      <  1:127126/140‑1 (MQ=255)

                                                              cGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTg                                                                 >  2:307983/1‑140 (MQ=255)

                                                                  aaGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAACCGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccac                                                             <  2:416204/140‑1 (MQ=255)

                                                                  aaGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccac                                                             <  2:454927/140‑1 (MQ=255)

                                                                  aaGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccac                                                             <  2:63861/140‑1 (MQ=255)

                                                                   aGCCAATCGAAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccacc                                                            <  2:216624/140‑1 (MQ=255)

                                                                        aTCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATccc                                                       <  1:153416/140‑1 (MQ=255)

                                                                                     agggccagggAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccacca                                                           <  2:56373/123‑1 (MQ=255)

                                                                                     agggccagggAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccacca                                                           >  1:56373/1‑123 (MQ=255)

                                                                                                       aTTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCa                        >  1:246643/1‑140 (MQ=255)

                                                                                                             cTGGGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGc                  <  2:503282/140‑1 (MQ=255)

                                                                                                             cTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGc                  <  1:277718/140‑1 (MQ=255)

                                                                                                                  gCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGccacca             >  2:259090/1‑140 (MQ=255)

                                                                                                                  gCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGccacca             >  2:468071/1‑140 (MQ=255)

                                                                                                                     gAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACgg          <  2:114585/140‑1 (MQ=255)

                                                                                                                             aaaaaaGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCtg  <  1:259090/140‑1 (MQ=255)

AAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTG  >  NC_000913/1023534‑1023798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A