Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	118	32.6	1094092	99.3%	1086433	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,210,492	A→G	E49E (GAA→GAG)	mcrA →	e14 prophage; 5‑methylcytosine‑specific restriction enzyme McrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,210,492	0	A	G	100.0%	137.8 / NA	39	E49E (GAA→GAG)	mcrA	e14 prophage; 5‑methylcytosine‑specific restriction enzyme McrA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/21); total (18/21)

TTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGATTGGTAATAGCCCCCG  >  NC_000913/1210356‑1210626

ttGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCgg                                                                                                                                     >  2:405001/1‑140 (MQ=255)

ttGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCgg                                                                                                                                     <  2:147227/140‑1 (MQ=255)

   ataataATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCggttg                                                                                                                                  <  2:333701/140‑1 (MQ=255)

     aataatGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCggttggt                                                                                                                                <  2:477230/140‑1 (MQ=255)

       taatGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGgttggttg                                                                                                                              >  2:138194/1‑140 (MQ=255)

          tGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGAtt                                                                                                                           >  1:152680/1‑140 (MQ=255)

          tGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGAtt                                                                                                                           >  1:323369/1‑140 (MQ=255)

          tGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGAtt                                                                                                                           <  1:342626/140‑1 (MQ=255)

          tGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGAtt                                                                                                                           <  1:455174/140‑1 (MQ=255)

               ttGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACGATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGgt                                                                                                                      >  2:388656/1‑140 (MQ=255)

                  aaCTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTa                                                                                                                   >  2:181802/1‑140 (MQ=255)

                   aCTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTAt                                                                                                                  >  1:225980/1‑140 (MQ=255)

                    cTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATc                                                                                                                 >  2:528476/1‑140 (MQ=255)

                       aaaGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCa                                                                                                              <  1:138194/140‑1 (MQ=255)

                         aGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAg                                                                                                            <  2:323369/140‑1 (MQ=255)

                          gCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGt                                                                                                           <  1:438362/140‑1 (MQ=255)

                           cTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTc                                                                                                          <  2:152680/140‑1 (MQ=255)

                                tgtTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCgtagt                                                                                                     <  1:405001/140‑1 (MQ=255)

                                  ttCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGtat                                                                                                   >  2:495426/1‑140 (MQ=255)

                                     gATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATc                                                                                                >  2:208751/1‑140 (MQ=255)

                                         ggTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGc                                                                                            <  2:253150/140‑1 (MQ=255)

                                            gAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGtc                                                                                         >  2:164245/1‑140 (MQ=255)

                                                   aTGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGtcatcatca                                                                                  <  1:495426/140‑1 (MQ=255)

                                                                tCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACata                                                                     >  1:499641/1‑140 (MQ=255)

                                                                    aTCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCa                                                                 <  1:407818/140‑1 (MQ=255)

                                                                               gTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATg                                                      >  1:377616/1‑140 (MQ=255)

                                                                                     gCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATgaaagaa                                                >  1:339805/1‑140 (MQ=255)

                                                                                       cGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGaaaa                                              >  1:447834/1‑140 (MQ=255)

                                                                                              cAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAatccatc                                       <  2:377616/140‑1 (MQ=255)

                                                                                                   aCAAAGATTACAATATCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGt                                  <  1:260848/140‑1 (MQ=255)

                                                                                                                tatCGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACtt                     <  1:388656/140‑1 (MQ=255)

                                                                                                                   cGCTCTTGATTATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGa                  <  1:181802/140‑1 (MQ=255)

                                                                                                                            ttATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGAt                 <  2:207896/132‑1 (MQ=255)

                                                                                                                            ttATATCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGAt                 >  1:207896/1‑132 (MQ=255)

                                                                                                                                atCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGATTGGTAATAGccc     >  2:210188/1‑140 (MQ=255)

                                                                                                                                atCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGATTGGTAATAGccc     >  1:529862/1‑140 (MQ=255)

                                                                                                                                 tCATTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGATTGGTAATAGcccc    <  2:499641/140‑1 (MQ=255)

                                                                                                                                   aTTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGATTGGTAATAGCCCCcg  <  1:210188/140‑1 (MQ=255)

                                                                                                                                   aTTGAGCGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGATTGGTAATAGCCCCcg  <  1:164245/140‑1 (MQ=255)

TTGATAATAATGGAATTGAACTGAAAGCTGAGTGTTCGATAGGTGAAGAGGATGGTGTTTATGGTCTAATCCTTGAGTCGTGGGGGCCGGGTGACAGAAACAAAGATTACAATATCGCTCTTGATTATATCATTGAACGGTTGGTTGATTCTGGTGTATCCCAAGTCGTAGTATATCTGGCGTCATCATCAGTCAGAAAACATATGCATTCTTTGGATGAAAGAAAAATCCATCCTGGTGAATATTTTACTTTGATTGGTAATAGCCCCCG  >  NC_000913/1210356‑1210626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A