Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F28 I1 R1
|
118 |
32.6 |
1094092 |
99.3% |
1086433 |
139.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,388,876 |
A→G |
intergenic (‑65/‑54) |
tpx ← / → ycjG |
lipid hydroperoxide peroxidase/L‑Ala‑D/L‑Glu epimerase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,388,876 | 0 | A | G | 100.0%
| 78.3
/ NA
| 23 | intergenic (‑65/‑54) | tpx/ycjG | lipid hydroperoxide peroxidase/L‑Ala‑D/L‑Glu epimerase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/11); total (12/11) |
CGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCG > NC_000913/1388749‑1389012
|
cGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTAtt < 2:199422/140‑1 (MQ=255)
gCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTAttt > 1:274947/1‑140 (MQ=255)
cTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTgc > 1:223986/1‑140 (MQ=255)
cTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATA‑AGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTgcg > 1:47242/1‑140 (MQ=255)
tGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATA‑AGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGac < 2:47242/140‑1 (MQ=255)
gCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGacc < 1:334325/140‑1 (MQ=255)
aCTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGaccacc < 2:168416/140‑1 (MQ=255)
ccTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTa < 2:274947/140‑1 (MQ=255)
aaCGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGaa > 1:280700/1‑140 (MQ=255)
aaCGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGaa < 2:211501/140‑1 (MQ=255)
ttGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTa > 1:82324/1‑140 (MQ=255)
tttCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGc < 2:223986/140‑1 (MQ=255)
tCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCt < 2:82324/140‑1 (MQ=255)
ccTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTg > 2:468631/1‑140 (MQ=255)
atatAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACa < 1:374174/140‑1 (MQ=255)
gTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATAccc > 1:246563/1‑140 (MQ=255)
tAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTgg > 1:37137/1‑125 (MQ=255)
tAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTgg < 2:37137/125‑1 (MQ=255)
aaCGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGt > 2:227917/1‑140 (MQ=255)
acacCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGa > 2:123332/1‑140 (MQ=255)
tGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGaa > 1:326228/1‑140 (MQ=255)
cTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGa > 1:456543/1‑133 (MQ=255)
cTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGa < 2:456543/133‑1 (MQ=255)
cacgGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAgcgcg > 2:326336/1‑140 (MQ=255)
|
CGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCG > NC_000913/1388749‑1389012
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
