Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I1 R1 28 562.2 19051950 96.7% 18423235 140.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,183,252 A→G G123G (GGT→GGC)  potC ← spermidine preferential ABC transporter membrane subunit PotC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,183,2520AG98.6% 2058.1 / 11.4 503G123G (GGT→GGC) potCspermidine preferential ABC transporter membrane subunit PotC
Reads supporting (aligned to +/- strand):  ref base A (6/1);  new base G (256/237);  total (264/239)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 504 total aligned reads displayed.

AATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGC  >  NC_000913/1183105‑1183396
                                                                                                                                                   |                                                                                                                                                
    gTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCcagc                                                                                                                                            <  2:2352304/150‑1 (MQ=255)
    gTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCcag                                                                                                                                             >  1:2352628/1‑149 (MQ=255)
       aaTTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCagcagc                                                                                                                                         <  1:2352371/150‑1 (MQ=255)
        aTTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAgcagca                                                                                                                                        >  1:2352636/1‑150 (MQ=255)
            gCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATaaa                                                                                                                                    <  1:2352164/150‑1 (MQ=255)
                gCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACa                                                                                                                                  <  2:2352106/148‑1 (MQ=255)
                gCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGc                                                                                                                                <  1:2351893/150‑1 (MQ=255)
                   ccGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCAc                                                                                                                              >  1:2352657/1‑149 (MQ=255)
                    cGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACca                                                                                                                            <  2:2352132/150‑1 (MQ=255)
                     gagaTCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACcag                                                                                                                           <  2:2352249/150‑1 (MQ=255)
                     gagaTCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACcag                                                                                                                           >  2:2352658/1‑150 (MQ=255)
                         tCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACcagca                                                                                                                         >  2:2352670/1‑148 (MQ=255)
                         tCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCagcagc                                                                                                                       >  1:2352670/1‑150 (MQ=255)
                             tCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGaa                                                                                                                    <  2:2352032/149‑1 (MQ=255)
                              cgccgcTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAAt                                                                                                                  >  1:2352680/1‑150 (MQ=255)
                               gccgcTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAAt                                                                                                                  <  1:2352238/149‑1 (MQ=255)
                               gccgcTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAAt                                                                                                                  >  2:2352682/1‑149 (MQ=255)
                                ccgcTTCCGGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATc                                                                                                                 <  2:2352407/149‑1 (MQ=255)
                                  gcTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGcc                                                                                                              <  1:2352446/150‑1 (MQ=255)
                                    ttCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCAt                                                                                                            >  1:2352690/1‑150 (MQ=255)
                                     tCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCAt                                                                                                            >  2:2352691/1‑149 (MQ=255)
                                       cAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCAc                                                                                                         <  1:2352179/150‑1 (MQ=255)
                                         gCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCCCGa                                                                                                       >  1:2352701/1‑150 (MQ=255)
                                           aTTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGata                                                                                                     <  1:2352006/150‑1 (MQ=255)
                                           aTTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGat                                                                                                      >  1:2352710/1‑149 (MQ=255)
                                            ttCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGatat                                                                                                    >  2:2352710/1‑150 (MQ=255)
                                            ttCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGatat                                                                                                    <  2:2352202/150‑1 (MQ=255)
                                               gCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGACTGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGccct                                                                                                            >  2:2352718/1‑137 (MQ=255)
                                                 aCGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGt                                                                                               <  1:2352415/150‑1 (MQ=255)
                                                   gTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCATAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAATAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGa                                                                                             <  2:2352325/150‑1 (MQ=255)
                                                      aaaaCCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGAcat                                                                                          >  2:2352727/1‑150 (MQ=255)
                                                       aaaCCTTTCAGGCGCGAATACACCGTCACCACGACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGAcatc                                                                                         <  1:2352356/150‑1 (MQ=255)
                                                        aaCCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGAcatca                                                                                        >  1:2352735/1‑150 (MQ=255)
                                                         aCCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGat                                                                                                      >  2:2352737/1‑135 (MQ=255)
                                                          ccTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGAcatcat                                                                                       >  1:2352743/1‑149 (MQ=255)
                                                               cAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATcacca                                                                                  >  1:2352753/1‑149 (MQ=255)
                                                                aGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATcacca                                                                                  >  2:2352753/1‑148 (MQ=255)
                                                                     cgAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAg                                                                           >  1:2352762/1‑150 (MQ=255)
                                                                       aaTACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCa                                                                         >  1:2352768/1‑150 (MQ=255)
                                                                        aTACACCGTCTCCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCa                                                                         <  1:2352401/149‑1 (MQ=255)
                                                                        aTACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCa                                                                         >  1:2352773/1‑149 (MQ=255)
                                                                         tACACCGTCACCACCACAAATGGCAGGCCGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAAGCGCCATCACGATATCTGGt                                                                                               >  2:2352776/1‑126 (MQ=255)
                                                                         tACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCAtt                                                                       <  2:2352362/150‑1 (MQ=255)
                                                                          acacCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATc                                                                                                           <  2:2351887/113‑1 (MQ=255)
                                                                           cacCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTcc                                                                     <  1:2352138/150‑1 (MQ=255)
                                                                           cacCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTc                                                                      >  1:2352780/1‑149 (MQ=255)
                                                                             ccGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGAcatc                                                                                         <  1:2352384/128‑1 (MQ=255)
                                                                              cGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCt                                                                  <  1:2352257/150‑1 (MQ=255)
                                                                              cGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCt                                                                  <  1:2352293/150‑1 (MQ=255)
                                                                               gTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTa                                                                 <  2:2352176/150‑1 (MQ=255)
                                                                                tCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaa                                                                >  1:2352789/1‑150 (MQ=255)
                                                                                 caccaccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATACCGCTaac                                                               >  2:2352793/1‑150 (MQ=255)
                                                                                  accaccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacg                                                              <  1:2352329/150‑1 (MQ=255)
                                                                                  accaccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaac                                                               <  1:2352430/149‑1 (MQ=255)
                                                                                    caccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGcc                                                                                                              >  2:2352800/1‑100 (MQ=255)
                                                                                    caccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacga                                                             <  1:2352046/149‑1 (MQ=255)
                                                                                      ccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCGGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGAGATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacgaacg                                                          <  2:2352347/150‑1 (MQ=255)
                                                                                      ccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacgaacg                                                          <  2:2352701/150‑1 (MQ=255)
                                                                                      ccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTaacgaacg                                                          <  2:2352418/150‑1 (MQ=255)
                                                                                      ccacAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATAACCAGCAGCATAAACAGCACCAGCAGCGaa                                                                                                                    >  2:2352805/1‑92 (MQ=255)
                                                                                       cacaAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACgg                                                         <  1:2352217/150‑1 (MQ=255)
                                                                                          aaaTGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACAtcacca                                                                                     >  1:2352815/1‑119 (MQ=255)
                                                                                          aaaTGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCtt                                                      <  1:2352347/150‑1 (MQ=255)
                                                                                          aaaTGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCt                                                       >  2:2352811/1‑149 (MQ=255)
                                                                                            aTGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTAc                                                    <  2:2352385/150‑1 (MQ=255)
                                                                                               gcaggcagAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCAc                                                 <  2:2352524/150‑1 (MQ=255)
                                                                                                 aggcagAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACg                                                >  2:2352820/1‑149 (MQ=255)
                                                                                                 aggcagAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACg                                                >  1:2352826/1‑149 (MQ=255)
                                                                                                   gcagACGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTa                                                                 <  2:2352272/130‑1 (MQ=255)
                                                                                                    cagAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAg                                            <  1:2352281/150‑1 (MQ=255)
                                                                                                     agAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGc                                           <  2:2352455/150‑1 (MQ=255)
                                                                                                       aaGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGa                                         >  1:2352834/1‑150 (MQ=255)
                                                                                                       aaGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGa                                         >  2:2352830/1‑150 (MQ=255)
                                                                                                           tGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAAc                                     >  1:2352844/1‑150 (MQ=255)
                                                                                                           tGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAAc                                     >  2:2352841/1‑150 (MQ=255)
                                                                                                             atatGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACgg                                   <  1:2352600/150‑1 (MQ=255)
                                                                                                              tatGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATCAACAGCACca                                                                                                                            >  2:2352852/1‑60 (MQ=255)
                                                                                                              tatGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACgg                                   >  1:2352853/1‑149 (MQ=255)
                                                                                                                  cGAGAACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAg                              >  1:2352862/1‑150 (MQ=255)
                                                                                                                   gagaACAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACTAAAGCGATAACGGTACAgc                             >  2:2352862/1‑150 (MQ=255)
                                                                                                                       aCAGCAGCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCa                          <  2:2352165/149‑1 (MQ=255)
                                                                                                                        cagcagCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCaa                         >  2:2352868/1‑149 (MQ=255)
                                                                                                                        cagcagCGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAAc                        <  1:2352723/150‑1 (MQ=255)
                                                                                                                         agcagcGACCAGAAGCCAAGCTGAATGCCCAGCAGCATTAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACt                       <  1:2352266/150‑1 (MQ=255)
                                                                                                                          gcagcGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTg                      <  1:2352465/150‑1 (MQ=255)
                                                                                                                           cagcGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCAGCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTg                      <  2:2352224/149‑1 (MQ=255)
                                                                                                                            agcGACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGcc                    >  2:2352875/1‑150 (MQ=255)
                                                                                                                               gACCAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTc                 <  1:2352319/150‑1 (MQ=255)
                                                                                                                                 ccAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGCTAACGGTACAGCGCAACTGCCGTCAg               >  1:2352882/1‑150 (MQ=255)
                                                                                                                                 ccAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAg               <  1:2352517/150‑1 (MQ=255)
                                                                                                                                  cAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAg               <  2:2352509/149‑1 (MQ=255)
                                                                                                                                  cAGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGt              <  1:2352309/150‑1 (MQ=255)
                                                                                                                                   aGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGt              >  2:2352887/1‑149 (MQ=255)
                                                                                                                                   aGAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGt              >  1:2352890/1‑149 (MQ=255)
                                                                                                                                    gAAGCCAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGa            <  2:2352610/150‑1 (MQ=255)
                                                                                                                                        ccAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAAcc         <  2:2352786/149‑1 (MQ=255)
                                                                                                                                        ccAAGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAAcc         >  1:2352899/1‑149 (MQ=255)
                                                                                                                                          aaGCTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGa       >  2:2352898/1‑149 (MQ=255)
                                                                                                                                             cTGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGAt      >  2:2352905/1‑147 (MQ=255)
                                                                                                                                              tGAATGCCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAg   >  1:2352910/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
AATGGTAAATTCGCTGGCACCGAGATCTTTCGCCGCTTCCAGCATTCGCACGTCAAAACCTTTCAGGCGCGAATACACCGTCACCACCACAAATGGCAGGCAGAAGGTGATATGCGAGAACAGCAGCGACCAGAAGCCAAGCTGAATACCCAGCAGCATAAACAGCACCAGCAGCGAAATCGCCATCACGATATCTGGTGACATCATCACCACAAACAGCATTCCGCTAACGAACGGCTTACCACGAAAGCGATAACGGTACAGCGCAACTGCCGTCAGTGAACCGATAAGC  >  NC_000913/1183105‑1183396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A