Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I1 R1 28 562.2 19051950 96.7% 18423235 140.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,554,784 (T)7→6 coding (886/1698 nt) maeA ← malate dehydrogenase, NAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,554,7780T.98.7% 2477.2 / 20.7 457coding (892/1698 nt)maeAmalate dehydrogenase, NAD‑requiring
Reads supporting (aligned to +/- strand):  ref base T (2/0);  major base . (238/210);  minor base C (4/2);  total (245/212)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 485 total aligned reads displayed.

GTTCGGCATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGA  >  NC_000913/1554629‑1554923
                                                                                                                                                     |                                                                                                                                                 
gTTCGGCATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAt                                                                                                                                                   >  2:3031570/1‑150 (MQ=255)
      cATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAtttttt                                                                                                                                              >  2:3031578/1‑149 (MQ=255)
       aTCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAttttttct                                                                                                                                            <  1:3030991/150‑3 (MQ=255)
          ttGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGc                                                                                                                                        >  1:3031584/1‑150 (MQ=255)
              cagtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTaa                                                                                                                                    >  2:3031587/1‑150 (MQ=255)
               agtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAAc                                                                                                                                   <  1:3031335/150‑1 (MQ=255)
                gtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAAc                                                                                                                                   >  2:3031594/1‑149 (MQ=255)
                gtcagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACt                                                                                                                                  <  2:3031092/150‑1 (MQ=255)
                  cagCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGa                                                                                                                                >  1:3031598/1‑150 (MQ=255)
                    gCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGacc                                                                                                                              <  1:3031051/150‑1 (MQ=255)
                     cAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGacca                                                                                                                             <  2:3031289/150‑1 (MQ=255)
                      aaGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGaccac                                                                                                                            >  1:3031604/1‑150 (MQ=255)
                       aGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGaccacc                                                                                                                           >  1:3031611/1‑150 (MQ=255)
                            aaGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCcg                                                                                                                      <  2:3030805/150‑1 (MQ=255)
                            aaGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCcg                                                                                                                      >  1:3031620/1‑150 (MQ=255)
                             aGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACG‑CTTTTTTCTCGCTTAACTGaccac                                                                                                                            >  2:3031619/1‑143 (MQ=255)
                                gATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGcc                                                                                                                       <  2:3031234/145‑1 (MQ=255)
                                gATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCcgcgcg                                                                                                                  <  1:3031095/150‑1 (MQ=255)
                                 aTCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCgg                                                                                                                 >  1:3031630/1‑150 (MQ=255)
                                 aTCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCAGcc                                                                                                                       >  2:3031629/1‑144 (MQ=255)
                                     aCCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtg                                                                                                             <  1:3030835/150‑1 (MQ=255)
                                        aTAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTtgctg                                                                                                          <  2:3031027/150‑1 (MQ=255)
                                           aaGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGa                                                                                                       >  1:3031640/1‑150 (MQ=255)
                                            aGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGAt                                                                                                      >  2:3031639/1‑150 (MQ=255)
                                               cTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAg                                                                                                   >  1:3031647/1‑150 (MQ=255)
                                                   cTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGAttttttt                                                                                                                                             >  2:3031654/1‑105 (MQ=255)
                                                   cTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGtgtg                                                                                               >  1:3031654/1‑150 (MQ=255)
                                                   cTGCCGCGCCGCTTCCTCGCTTAACCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCt                                                                                                                          <  2:3031220/123‑1 (MQ=255)
                                                    tGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAgtgt                                                                                                <  2:3031204/148‑1 (MQ=255)
                                                        gcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGTCCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGAc                                                                                          >  1:3031665/1‑150 (MQ=255)
                                                        gcgccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGAc                                                                                          >  2:3031664/1‑150 (MQ=255)
                                                          gccgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTg                                                                                        <  2:3031072/150‑1 (MQ=255)
                                                            cgcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGt                                                                                       >  1:3031673/1‑149 (MQ=255)
                                                             gcTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTa                                                                                     >  1:3031679/1‑150 (MQ=255)
                                                                 ccTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgc                                                                                 >  1:3031686/1‑150 (MQ=255)
                                                                 ccTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccg                                                                                  <  1:3031213/149‑1 (MQ=255)
                                                                  cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgcc                                                                                <  1:3031313/150‑1 (MQ=255)
                                                                  cTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgc                                                                                 >  2:3031686/1‑149 (MQ=255)
                                                                     gCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgccg                                                                               >  1:3031693/1‑148 (MQ=255)
                                                                     gCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCa                                                                             <  1:3031398/150‑1 (MQ=255)
                                                                     gCTTAATCCTTCGCGCTGGGTCTAGGAGATGATAATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTAccgcc                                                                                >  2:3031693/1‑147 (MQ=255)
                                                                        tAATCCTTCGCGCTGGGTCTGGGCGATGATCATGTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTg                                                                          >  2:3031699/1‑150 (MQ=255)
                                                                         aaTCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGc                                                                         >  1:3031704/1‑150 (MQ=255)
                                                                          aTCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGc                                                                         <  1:3031135/149‑1 (MQ=255)
                                                                               tCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACt                                                                                         >  2:3031712/1‑128 (MQ=255)
                                                                               tCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGaa                                                                   >  1:3031713/1‑150 (MQ=255)
                                                                                 gcgcTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAAt                                                                  <  1:3031281/149‑1 (MQ=255)
                                                                                    cTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTg                                                                     >  2:3031721/1‑143 (MQ=255)
                                                                                    cTGGGTCTGGGAGAGGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAGGGAAGANGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTc                                                               <  1:3031255/149‑1 (MQ=255)
                                                                                     tGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCAt                                                             >  1:3031724/1‑150 (MQ=255)
                                                                                       ggTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATc                                                            <  2:3031249/149‑1 (MQ=255)
                                                                                       ggTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCg                                                           <  1:3031154/150‑1 (MQ=255)
                                                                                       ggTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCg                                                           <  2:3031355/150‑1 (MQ=255)
                                                                                        gTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGt                                                          <  1:3031364/150‑1 (MQ=255)
                                                                                         tCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGtt                                                         <  1:3031495/150‑1 (MQ=255)
                                                                                         tCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGt                                                          >  1:3031730/1‑149 (MQ=255)
                                                                                         tCTGGGAGATGACCATTTCGGCAATGCCGCATCCCGCTGACCCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTCCCCTGAATGTCATCGtt                                                         <  2:3031170/150‑1 (MQ=255)
                                                                                            gggAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGt                                                                           <  2:3031141/129‑1 (MQ=255)
                                                                                              gagaTGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTTACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTccc                                                                        >  2:3031736/1‑130 (MQ=255)
                                                                                               agaTGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAg                                                    >  1:3031739/1‑149 (MQ=255)
                                                                                               agaTGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACAGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGa                                                   <  2:3031376/150‑1 (MQ=255)
                                                                                                gatgatCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCCCCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGaa                                                  <  1:3031349/150‑1 (MQ=255)
                                                                                                    atCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACaa                                               >  1:3031747/1‑149 (MQ=255)
                                                                                                    atCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACa                                                <  1:3031388/148‑1 (MQ=255)
                                                                                                       aTTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGCCCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCAt                                                             <  2:3030921/132‑1 (MQ=255)
                                                                                                       aTTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                           <  1:3031268/150‑1 (MQ=255)
                                                                                                       aTTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                           <  1:3030931/150‑1 (MQ=255)
                                                                                                       aTTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                           <  2:3031268/150‑1 (MQ=255)
                                                                                                       aTTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAtt                                            <  1:3031071/149‑1 (MQ=255)
                                                                                                        tttCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCTGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                           >  1:3031754/1‑149 (MQ=255)
                                                                                                        tttCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAAttt                                           >  2:3031750/1‑149 (MQ=255)
                                                                                                         ttCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTg                                                                     >  2:3031757/1‑122 (MQ=255)
                                                                                                           cGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCcgc                                                                                                                     <  1:3031412/72‑1 (MQ=255)
                                                                                                            ggCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTg                                      <  1:3031292/150‑1 (MQ=255)
                                                                                                             gCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGc                                     >  1:3031765/1‑150 (MQ=255)
                                                                                                                    cGCATCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCTCTTTACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTCCCCTGAATGTCCTCGTTAAAAGCACaa                                               >  2:3031767/1‑133 (MQ=255)
                                                                                                                       aTCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGtt                           <  2:3031608/150‑1 (MQ=255)
                                                                                                                       aTCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGtt                           <  1:3031326/150‑1 (MQ=255)
                                                                                                                       aTCCCGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGt                            >  2:3031775/1‑149 (MQ=255)
                                                                                                                          ccGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTaa                         <  2:3031519/149‑1 (MQ=255)
                                                                                                                          ccGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTaa                         <  1:3031169/149‑1 (MQ=255)
                                                                                                                          ccGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAg                        >  1:3031782/1‑150 (MQ=255)
                                                                                                                           cGCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCg                              >  2:3031785/1‑143 (MQ=255)
                                                                                                                            gCTGAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGt                       >  1:3031788/1‑149 (MQ=255)
                                                                                                                             cTGAACCTGCGCCAAGGAAGATGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTaa                     <  1:3031233/150‑1 (MQ=255)
                                                                                                                               gAACCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACg                   >  1:3031797/1‑150 (MQ=255)
                                                                                                                                aaCCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACgg                  >  2:3031797/1‑150 (MQ=255)
                                                                                                                                aaCCTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACg                   <  2:3031466/149‑1 (MQ=255)
                                                                                                                                  ccTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCa                <  1:3031433/150‑1 (MQ=255)
                                                                                                                                   cTGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCAt               <  1:3031472/150‑1 (MQ=255)
                                                                                                                                   cTGCGCCAAGGAAGACGA‑TTTTTGCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTaaa                                                      <  2:3031341/111‑1 (MQ=255)
                                                                                                                                    tGCGCCAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCNGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATc              >  1:3031804/1‑150 (MQ=255)
                                                                                                                                        ccAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGAt                                  <  2:3031423/126‑1 (MQ=255)
                                                                                                                                        ccAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAt          <  1:3031533/150‑1 (MQ=255)
                                                                                                                                        ccAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAt          >  2:3031809/1‑150 (MQ=255)
                                                                                                                                         cAAGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAtt         >  1:3031815/1‑150 (MQ=255)
                                                                                                                                          aaGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAttt        >  1:3031821/1‑150 (MQ=255)
                                                                                                                                          aaGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAttt        >  2:3031821/1‑150 (MQ=255)
                                                                                                                                           aGGAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCAtttt       >  1:3031829/1‑150 (MQ=255)
                                                                                                                                             gAAGACGA‑TTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGc            <  2:3031316/143‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GTTCGGCATCTTGTCAGTCAGCAAGCCAAAGCGATCGACCATAAAGACTTTCTGCCGCGCCGCTTCCTCGCTTAATCCTTCGCGCTGGGTCTGGGAGATGATCATTTCGGCAATGCCGCATCCCGCTGAACCTGCGCCAAGGAAGACGATTTTTTTCTCGCTTAACTGACCACCTGCCGCGCGGCTTGCTGCGATCAGTGTGCCGACTGTTACCGCCGCAGTGCCCTGAATGTCATCGTTAAAAGAACAAATTTCATTGCGATAGCGGTTAAGTAACGGCATCGCATTTTTTTGA  >  NC_000913/1554629‑1554923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A