Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F13 I1 R1 28 562.2 19051950 96.7% 18423235 140.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,465,686 (A)8→7 coding (144/678 nt) gspO → Type II secretion system prepilin peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,465,6790A.89.6% 2114.8 / 270.2 489coding (137/678 nt)gspOType II secretion system prepilin peptidase
Reads supporting (aligned to +/- strand):  ref base A (17/34);  new base . (236/202);  total (253/236)
Fisher's exact test for biased strand distribution p-value = 7.25e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 527 total aligned reads displayed.

GGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTT  >  NC_000913/3465530‑3465827
                                                                                                                                                     |                                                                                                                                                    
  gAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCaa                                                                                                                                                     >  1:6771333/1‑149 (MQ=255)
     caacaaTAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCaaaaa                                                                                                                                                  >  2:6771337/1‑149 (MQ=255)
        caaTAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCaaaaaaata                                                                                                                                              >  1:6771342/1‑148 (MQ=255)
         aataatGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCaaaaaaatat                                                                                                                                             <  1:6770877/150‑4 (MQ=255)
             atGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCt                                                                                                                                        >  1:6771353/1‑150 (MQ=255)
                aCAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTgg                                                                                                                                      >  1:6771359/1‑149 (MQ=255)
                    tGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGa                                                                                                                                 >  1:6771368/1‑150 (MQ=255)
                         cTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAg                                                                                                                            >  2:6771372/1‑150 (MQ=255)
                          tGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGt                                                                                                                           >  1:6771377/1‑150 (MQ=255)
                            ccGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGt                                                                                                                           <  1:6770632/148‑1 (MQ=255)
                               ctctTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGcc                                                                                                                        >  1:6771388/1‑148 (MQ=255)
                                 ctTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTATCGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCt                                                                                                                     >  2:6771387/1‑149 (MQ=255)
                                  ttCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCt                                                                                                                     <  1:6770961/148‑1 (MQ=255)
                                    cATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGcccc                                                                                                                      <  1:6771029/146‑1 (MQ=255)
                                    cATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATa                                                                                                                 >  1:6771397/1‑150 (MQ=255)
                                    cATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCAAATCA‑AAAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGAt                                                                                                                  <  2:6771070/149‑1 (MQ=255)
                                     aTTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTACCGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCt                                                                                                                                        >  2:6771398/1‑127 (MQ=255)
                                     aTTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATa                                                                                                                 <  1:6771088/149‑1 (MQ=255)
                                     aTTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATAc                                                                                                                <  1:6770811/150‑1 (MQ=255)
                                          ggttggttTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAGAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTtgt                                                                                                           >  2:6771407/1‑150 (MQ=255)
                                          ggttggttTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTtgt                                                                                                           >  1:6771410/1‑150 (MQ=255)
                                           gttggttTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATCCAGTGCCCCTgc                                                                                                                   >  1:6771418/1‑140 (MQ=255)
                                               gttTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgc                                                                                                      <  1:189584/150‑1 (MQ=255)
                                               gttTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtg                                                                                                       <  1:6770982/149‑1 (MQ=255)
                                                 tttATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGACGTTGGAGGAGAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgct                                                                                                     >  2:6771423/1‑149 (MQ=255)
                                                   tATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgctgct                                                                                                  >  1:6771429/1‑150 (MQ=255)
                                                    aTTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgctgc                                                                                                   <  1:6770926/149‑1 (MQ=255)
                                                    aTTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTtgctgc                                                                                                   >  2:6771431/1‑148 (MQ=255)
                                                       gCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTgctgctgct                                                                                               <  1:6770696/149‑1 (MQ=255)
                                                        cAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGct                                                                                                                                         >  2:6771443/1‑105 (MQ=255)
                                                        cAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTAt                                                                                             >  1:6771443/1‑150 (MQ=255)
                                                        cAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTAt                                                                                             <  1:6770761/150‑1 (MQ=255)
                                                        cAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTAt                                                                                             <  1:6770742/150‑1 (MQ=255)
                                                          gATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCg                                                                                           >  1:6771450/1‑150 (MQ=255)
                                                            ttattTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGcc                                                                                         <  1:6770849/150‑1 (MQ=255)
                                                              attTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTg                                                                                       >  1:6771455/1‑150 (MQ=255)
                                                               ttttGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCt                                                                                        <  1:6770792/149‑1 (MQ=255)
                                                                tttGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTgc                                                                                      <  1:6770776/149‑1 (MQ=255)
                                                                 ttGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTgcg                                                                                     <  2:6770902/149‑1 (MQ=255)
                                                                   gTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGccc                                                                                  >  1:6771464/1‑150 (MQ=255)
                                                                      aaTGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTgg                                                                               >  1:6771470/1‑150 (MQ=255)
                                                                      aaTGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTg                                                                                <  1:6771209/149‑1 (MQ=255)
                                                                      aaTGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTg                                                                                <  1:6770672/149‑1 (MQ=255)
                                                                       aTGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTgg                                                                               >  2:6771471/1‑149 (MQ=255)
                                                                        tGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCt                                                                                                                                        <  1:6771231/91‑1 (MQ=255)
                                                                          ccATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCAcc                                                                           >  1:6771480/1‑150 (MQ=255)
                                                                           cATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGACTGCGCCCTGGCACCt                                                                          >  2:6771485/1‑150 (MQ=255)
                                                                            aTCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGAGCCCCTGATACTTTGTATTGCAGCTGCTATCGCCTGCGCCCTGGCACCtt                                                                         >  1:6771489/1‑150 (MQ=255)
                                                                             tCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCt                                                                                 <  2:6771124/141‑1 (MQ=255)
                                                                                ccTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCaaaaca                                                                                                                                                 >  2:6771495/1‑73 (MQ=255)
                                                                                 cTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACg                                                                    >  1:6771499/1‑150 (MQ=255)
                                                                                   aTCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTTTGTTGCTGCTGCTATCGCCTGCGccc                                                                                  >  1:6771511/1‑135 (MQ=255)
                                                                                        ctctCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCg                                                             <  1:6770579/150‑1 (MQ=255)
                                                                                        ctctCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGACCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACg                                                                    >  2:6771511/1‑143 (MQ=255)
                                                                                           tcGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGTCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGt                                                            <  1:6770900/148‑1 (MQ=255)
                                                                                             gCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGAc                                                         <  1:6771117/149‑1 (MQ=255)
                                                                                             gCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGAc                                                         <  1:6770939/149‑1 (MQ=255)
                                                                                              ccGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACt                                                        <  2:6771352/149‑1 (MQ=255)
                                                                                              ccGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTg                                                       >  1:6771521/1‑150 (MQ=255)
                                                                                               cGTTGGAGGATACAACGGCGTTAACTTTTCGCCAGGTATTGGTTCATGTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGccc                                                                 <  2:6770619/139‑1 (MQ=255)
                                                                                               cGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGt                                                            <  2:6770725/145‑1 (MQ=255)
                                                                                                gTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGgc                                                     >  2:6771522/1‑150 (MQ=255)
                                                                                                  gggaggaTAAAACGGCGTTAACGTTTCGCCAGGTATTGGTGCATGACAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCAt                                                               <  2:6771143/137‑1 (MQ=255)
                                                                                                    gaggaCAAAAAGGCGTTAACGTTTCGCCATTTTTTGGTTAAAAAAA‑AAAAAAAAAATATGCCTGGCATGAt                                                                                                                                <  2:6770859/71‑1 (MQ=255)
                                                                                                     aggaTAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGTCTGGCGCAct                                                >  1:6771529/1‑150 (MQ=255)
                                                                                                     aggaTAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCAct                                                <  1:6771151/150‑1 (MQ=255)
                                                                                                     aggaCAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTAAAAAAAAAAAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATc                                                                                            <  2:6770802/107‑1 (MQ=255)
                                                                                                        aTAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCtt                                             <  2:6771264/150‑1 (MQ=255)
                                                                                                        aTAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCANGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCtt                                             <  1:6770948/150‑1 (MQ=255)
                                                                                                          aaaaCGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCttt                                            >  2:6771535/1‑149 (MQ=255)
                                                                                                            aaCGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTtctc                                         >  1:6771540/1‑150 (MQ=255)
                                                                                                              cGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTa                                       >  1:6771548/1‑150 (MQ=255)
                                                                                                               ggCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCt                                                                                 <  2:6771053/107‑1 (MQ=255)
                                                                                                               ggCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGgcg                                                    >  2:6771548/1‑136 (MQ=255)
                                                                                                               ggCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTAt                                      <  1:6771045/150‑1 (MQ=255)
                                                                                                               ggCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTAt                                      <  1:6771188/150‑1 (MQ=255)
                                                                                                                 cGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTAttt                                    <  1:6771000/150‑1 (MQ=255)
                                                                                                                    tAACGTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTg                                 >  2:6771554/1‑150 (MQ=255)
                                                                                                                     aaCGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGCCTGGCGCACTCTTTCTCTATTTCTg                                 >  1:6771559/1‑150 (MQ=255)
                                                                                                                        gTTTCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTgg                                                                               <  2:6770929/100‑1 (MQ=255)
                                                                                                                          ttCGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACt                                                        <  2:6771170/121‑1 (MQ=255)
                                                                                                                          tgcGCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGcc                                                                                                                        <  2:6771029/55‑1 (MQ=255)
                                                                                                                             gCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTAc                         <  1:6770834/150‑1 (MQ=255)
                                                                                                                             gCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTNTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACt                        <  1:6771075/150‑1 (MQ=255)
                                                                                                                             gCCAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTCGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCa                                                                <  2:6771013/110‑1 (MQ=255)
                                                                                                                               cAGGTATTGGTTCATTTCAGGC‑CAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTCCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCAATCGTGACTGGCGCACTCTTtct                                          >  2:6771569/1‑130 (MQ=255)
                                                                                                                               cAGGTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCa                      >  1:6771568/1‑150 (MQ=255)
                                                                                                                                 ggTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCAc                     <  1:6771099/149‑1 (MQ=255)
                                                                                                                                 ggTATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACg                    >  1:6771579/1‑150 (MQ=255)
                                                                                                                                   tATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACGCt                  <  2:6771200/150‑1 (MQ=255)
                                                                                                                                   tATTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACGCt                  >  1:6771585/1‑150 (MQ=255)
                                                                                                                                    aTTGGTTCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTTACGc                   >  2:6771587/1‑148 (MQ=255)
                                                                                                                                      tGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACGCt                  >  1:6771592/1‑148 (MQ=255)
                                                                                                                                         ttCATTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACGCTCAgtgt            >  1:6771599/1‑150 (MQ=255)
                                                                                                                                           cATTTCAGGC‑AAAAAAATATGCCTGGCCTGATACAGTGCCCCTGATAATTTGTGTTGCTGCGACTATCGCCTGCGCCCTGGCATCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCAATTTCTGTTTCGTACTCCCGCTCAGTGttc          >  2:6771602/1‑149 (MQ=255)
                                                                                                                                            aTTTCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTAt         >  1:6771609/1‑150 (MQ=255)
                                                                                                                                             tttCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTtg                                                                                                            <  2:6770770/50‑1 (MQ=255)
                                                                                                                                             tttCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTAt                                      >  2:6771612/1‑120 (MQ=255)
                                                                                                                                               tCAGGC‑AAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGa      >  1:6771618/1‑150 (MQ=255)
                                                                                                                                                  aaaaaaaaaaaTATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTgcg                                                                                     <  2:6770953/66‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGGAGCAACAATAATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGATTATTTTGTTAATGCCATCGCCTATCACCTCTCGCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATACAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTCTTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTT  >  NC_000913/3465530‑3465827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A