Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F13 I1 R1 18 2625.4 86379875 99.4% 85861595 141.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,965,557 (T)8→7 coding (74/1266 nt) rhlB ← ATP‑dependent RNA helicase RhlB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,965,5500T.98.1% 12706.0 / 227.6 2313coding (81/1266 nt)rhlBATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base T (31/12);  new base . (925/1338);  total (958/1355)
Fisher's exact test for biased strand distribution p-value = 5.80e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2679 total aligned reads displayed.

ATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATAAAGTCAACCT  >  NC_000913/3965401‑3965695
                                                                                                                                                     |                                                                                                                                                 
   gCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCttt                                                                                                                                                 <  2:36340599/149‑1 (MQ=255)
    cAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCttttt                                                                                                                                               >  1:36342332/1‑150 (MQ=255)
     aGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttt                                                                                                                                              >  2:36342353/1‑150 (MQ=255)
       gATGAGAGAGAAGATAATTAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttttc                                                                                                                                            <  2:36340695/150‑2 (MQ=255)
       gATGAGAGAGAAGATAATGAAACGGTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttttc                                                                                                                                            <  1:36338664/150‑2 (MQ=255)
        aTGAGAGAGAAGATAATGAAACGGTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttttca                                                                                                                                           <  2:36340405/150‑3 (MQ=255)
         tGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttttcaa                                                                                                                                          <  2:36342234/150‑4 (MQ=255)
           agagagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAgg                                                                                                                                       >  2:36342421/1‑150 (MQ=255)
             agagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAggg                                                                                                                                      <  1:36340525/149‑1 (MQ=255)
             agagaAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGc                                                                                                                                     >  1:36342441/1‑150 (MQ=255)
                gaagaTAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTc                                                                                                                                  <  2:36340120/150‑1 (MQ=255)
                 aagaTAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTc                                                                                                                                  >  1:36342490/1‑149 (MQ=255)
                   gaTAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTAc                                                                                                                               <  1:36341511/150‑1 (MQ=255)
                      aaTGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCttttt                                                                                                                                               >  2:36342548/1‑132 (MQ=255)
                      aaTGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAAc                                                                                                                            >  1:36342547/1‑150 (MQ=255)
                       aTGAAACTTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAAcc                                                                                                                           <  2:36339949/150‑1 (MQ=255)
                        tGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCt                                                                                                                          <  1:36336624/150‑1 (MQ=255)
                          aaaCGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTc                                                                                                                        >  1:36342597/1‑150 (MQ=255)
                            aCGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttt                                                                                                                                              >  2:36342616/1‑127 (MQ=255)
                               ttGACGTAAGCAACGCCATCGTTTGCCCGGTACCGGGTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGAt                                                                                                                    <  2:36338951/149‑1 (MQ=255)
                               ttGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATg                                                                                                                   >  1:36342658/1‑150 (MQ=255)
                                tGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGAt                                                                                                                    <  1:36339723/148‑1 (MQ=255)
                                 gACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATg                                                                                                                   >  2:36342671/1‑148 (MQ=255)
                                   cGTAAGAAACGCCAGCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAgg                                                                                                               <  1:36340796/150‑1 (MQ=255)
                                    gTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAggg                                                                                                              >  1:36342705/1‑150 (MQ=255)
                                     tAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGc                                                                                                             <  2:36339494/150‑1 (MQ=255)
                                      aaGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGc                                                                                                             >  2:36342728/1‑149 (MQ=255)
                                        gAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGaa                                                                                                          <  1:36340946/150‑1 (MQ=255)
                                        gAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGaa                                                                                                          <  1:36340111/150‑1 (MQ=255)
                                         aaaCGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGGTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAg                                                                                                         >  1:36342760/1‑150 (MQ=255)
                                            cGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCg                                                                                                      <  1:36340703/150‑1 (MQ=255)
                                            cGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCg                                                                                                      >  2:36342794/1‑150 (MQ=255)
                                             gCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCGCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTc                                                                                                                        <  2:36337494/131‑1 (MQ=255)
                                             gCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCg                                                                                                      >  1:36342810/1‑149 (MQ=255)
                                              ccATCGGTTTCCCGGTACCGGTTGGCGCCTGCCCCGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAg                                                                                                         <  1:36341354/145‑1 (MQ=255)
                                               cATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttttc                                                                                                                                            >  2:36342845/1‑109 (MQ=255)
                                                  cGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAAc                                                                                                >  1:36342865/1‑150 (MQ=255)
                                                     tttCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACGTTCGGATGCAGGGCGAAGTCGGAAAACttc                                                                                             >  2:36342902/1‑150 (MQ=255)
                                                         ccGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgtt                                                                                         <  1:36340263/150‑1 (MQ=255)
                                                         ccGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgtt                                                                                         <  2:36339313/150‑1 (MQ=255)
                                                          cGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGAt                                                                                                                    >  2:36342939/1‑122 (MQ=255)
                                                          cGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaa                                                                                                   <  2:36339182/139‑1 (MQ=255)
                                                           ggTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct                                                                                       <  2:36340999/150‑1 (MQ=255)
                                                           ggTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct                                                                                       <  2:36340778/150‑1 (MQ=255)
                                                           ggTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct                                                                                       >  1:36342945/1‑150 (MQ=255)
                                                             tACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTtctgttctgt                                                                                     <  1:36339875/150‑1 (MQ=255)
                                                               ccGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTa                                                                                   <  2:36341556/150‑1 (MQ=255)
                                                                 ggTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttt                                                                                                                                              >  2:36342988/1‑90 (MQ=255)
                                                                      gcgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAtgtg                                                                             <  1:36339574/149‑1 (MQ=255)
                                                                      gcgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATgtgt                                                                            >  1:36343012/1‑150 (MQ=255)
                                                                       cgcCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttc                                                                                        <  2:36339782/137‑1 (MQ=255)
                                                                         ccTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGtttt                                                                         >  2:36343033/1‑150 (MQ=255)
                                                                          cTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTg                                                                        <  2:36341076/150‑1 (MQ=255)
                                                                            gCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCt                                                                      >  1:36343057/1‑150 (MQ=255)
                                                                             cccGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttct                                                                                            <  2:36340312/127‑1 (MQ=255)
                                                                              ccGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGc                                                                       <  2:36341308/147‑1 (MQ=255)
                                                                              ccGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCa                                                                    <  1:36340615/150‑1 (MQ=255)
                                                                                 gCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAg                                                                 <  1:36339068/150‑1 (MQ=255)
                                                                                    aCGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaaaa                                                                                                 >  2:36343102/1‑115 (MQ=255)
                                                                                     cGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGtgtg                                                             >  1:36343109/1‑150 (MQ=255)
                                                                                     cGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTACGGATGCAGGGCGAAGTCGGAAAACttct                                                                                            <  2:36338599/119‑1 (MQ=255)
                                                                                      ggcACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAgtgt                                                              <  2:36339879/146‑1 (MQ=255)
                                                                                          cGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGa                                                        <  1:36339290/150‑1 (MQ=255)
                                                                                                gCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAatat                                                    >  1:36343151/1‑148 (MQ=255)
                                                                                                gCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCa                                                  <  2:36340212/150‑1 (MQ=255)
                                                                                                   aGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGc                                                <  2:36341189/149‑1 (MQ=255)
                                                                                                   aGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCt                                               >  2:36343171/1‑150 (MQ=255)
                                                                                                     cGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCATGGCGAAGTCGGAAAACCTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAg                                                         <  2:36340028/138‑1 (MQ=255)
                                                                                                      gTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGaa                                                       <  1:36341785/139‑1 (MQ=255)
                                                                                                       tCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGNGGTAGAATATCAGCTTACt                                           <  2:36341452/150‑1 (MQ=255)
                                                                                                         aGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTAt                                         <  2:36340495/150‑1 (MQ=255)
                                                                                                         aGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATAACAGCTTACTAt                                         >  1:36343204/1‑150 (MQ=255)
                                                                                                            ggAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGc                                      <  1:36339997/150‑1 (MQ=255)
                                                                                                             gAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttt                                                                                                                                              >  2:36343236/1‑46 (MQ=255)
                                                                                                              aaGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCtttttttc                                                                                                                                            >  1:36343244/1‑46 (MQ=255)
                                                                                                                     aGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGaaa                             <  2:36340892/150‑1 (MQ=255)
                                                                                                                     aGTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGaaa                             <  2:36339594/150‑1 (MQ=255)
                                                                                                                      gTGCCTGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAg                            >  1:36343290/1‑150 (MQ=255)
                                                                                                                        gCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTT‑TTTTTTTTTTACAACCTTCGGATGCAggg                                                                                                              >  2:36343307/1‑66 (MQ=255)
                                                                                                                           tGAATGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTAt                       >  1:36343329/1‑150 (MQ=255)
                                                                                                                             aaTGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATc                      <  1:36340179/149‑1 (MQ=255)
                                                                                                                               tGGGCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCgg                   <  1:36342174/150‑1 (MQ=255)
                                                                                                                                 ggCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTg                 <  2:36342493/150‑1 (MQ=255)
                                                                                                                                 ggCGTACAGTTAGGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGt                  >  2:36343377/1‑149 (MQ=255)
                                                                                                                                  gCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTa                                  <  2:36339415/132‑1 (MQ=255)
                                                                                                                                  gCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGt                  <  2:36339703/148‑1 (MQ=255)
                                                                                                                                  gCGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGa                <  1:36340849/150‑1 (MQ=255)
                                                                                                                                   cGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTg                                       <  1:36340346/126‑1 (MQ=255)
                                                                                                                                   cGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTg                 <  1:36341158/148‑1 (MQ=255)
                                                                                                                                   cGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGaa               <  1:36341053/150‑1 (MQ=255)
                                                                                                                                   cGTACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGa                >  1:36343387/1‑149 (MQ=255)
                                                                                                                                     tACAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGaaa              >  1:36343418/1‑149 (MQ=255)
                                                                                                                                       cAGTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATa            <  1:36340416/149‑1 (MQ=255)
                                                                                                                                         gTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACg                                >  2:36343459/1‑127 (MQ=255)
                                                                                                                                         gTTATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATAAAg         <  2:36341905/150‑1 (MQ=255)
                                                                                                                                          ttATGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATAAAGt        >  1:36343465/1‑150 (MQ=255)
                                                                                                                                            aTGAAACCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATAAAGTc       >  1:36343497/1‑149 (MQ=255)
                                                                                                                                               aaaCCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATAAAGTCAAcc   <  1:36342388/150‑1 (MQ=255)
                                                                                                                                               aaaCCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATAAAGTCAAc    <  1:36341247/149‑1 (MQ=255)
                                                                                                                                                aaCCC‑TTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATAAAGTCAACCt  <  2:36341683/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATCGCAGGATGAGAGAGAAGATAATGAAACGTTGACGTAAGAAACGCCATCGTTTTCCCGGTACCGGTTTGCGCCTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATGTGTTTTGCTCATAGTGTGGTAGAATATCAGCTTACTATTGCTTTACGAAAGCGTATCCGGTGAAATAAAGTCAACCT  >  NC_000913/3965401‑3965695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A