Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F13 I1 R1 15 2083.3 68373553 99.6% 68100058 141.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,979,176 T→C T14A (ACT→GCT)  insA‑5 ← IS1 protein InsA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,979,1760TC99.6% 2632.9 / 0.9 677T14A (ACT→GCT) insA‑5IS1 protein InsA
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base C (278/396);  total (281/396)
Fisher's exact test for biased strand distribution p-value = 7.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

Only 100 of 685 total aligned reads displayed.

GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAA  >  NC_000913/1979123‑1979321
                                                     |                                                                                                                                                 
 agaTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACCCCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCAt                                                  <  2:14458630/150‑1 (MQ=17)
 agaTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCa                                                   <  2:14460418/149‑1 (MQ=14)
 agaTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCa                                                   >  2:14462155/1‑149 (MQ=14)
 agaTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCa                                                   >  1:14462157/1‑149 (MQ=14)
   aTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATaa                                                <  1:14459072/150‑1 (MQ=18)
   aTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATaa                                                <  1:14461219/150‑1 (MQ=18)
   aTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATa                                                 >  2:14462166/1‑149 (MQ=17)
    tAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATaaa                                               <  1:14458970/150‑1 (MQ=21)
    tAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATaaa                                               >  1:14462172/1‑150 (MQ=21)
    tAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATaaa                                               <  1:14460334/150‑1 (MQ=21)
     aGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAAt                                              >  2:14462178/1‑150 (MQ=25)
      gcgcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAAt                                              <  2:14459859/149‑1 (MQ=25)
      gcgcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAAt                                              <  1:14460926/149‑1 (MQ=25)
      gcgcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAAt                                              <  1:14460055/149‑1 (MQ=25)
      gcgcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATg                                             >  1:14462186/1‑150 (MQ=25)
      gcgcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATg                                             <  1:14460874/150‑1 (MQ=25)
        gcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGta                                           >  1:14462206/1‑150 (MQ=38)
        gcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGta                                           >  1:14462198/1‑150 (MQ=38)
        gcTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGta                                           <  2:14460178/150‑1 (MQ=38)
         cTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGt                                            >  2:14462217/1‑148 (MQ=37)
         cTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTCAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGta                                           >  1:14462216/1‑149 (MQ=255)
           gATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGtat                                          >  1:14462228/1‑148 (MQ=255)
            aTGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATaa                                        <  1:14458038/149‑1 (MQ=255)
             tGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATaa                                        >  2:14462237/1‑148 (MQ=255)
             tGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGt                                      >  1:14462235/1‑150 (MQ=255)
             tGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGt                                      <  1:14459901/150‑1 (MQ=255)
              gTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGt                                      <  2:14459933/149‑1 (MQ=255)
              gTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGt                                      >  1:14462244/1‑149 (MQ=255)
              gTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGt                                      <  1:14459554/149‑1 (MQ=255)
               tCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCa                                                   >  2:14462253/1‑135 (MQ=14)
               tCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTc                                     <  2:14460653/149‑1 (MQ=255)
               tCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCa                                    <  1:14460763/150‑1 (MQ=255)
               tCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCa                                    <  1:14460515/150‑1 (MQ=255)
                ccGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCa                                    <  2:14461100/149‑1 (MQ=255)
                 cggctgTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTc                                     <  1:14460651/147‑1 (MQ=255)
                 cggcggTGCTTTTGCCGTTACGCACCACCCCGTCGGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATa                                  >  1:14462267/1‑150 (MQ=255)
                 cggcggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTGAGCTGGCAGCATCACCCAt                                                  <  2:14460081/134‑1 (MQ=12)
                 cggcggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCAt                                   <  1:14459403/149‑1 (MQ=255)
                 cggcggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATa                                  >  2:14462264/1‑150 (MQ=255)
                 cggcggTGCTTTTGCCGGTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCAt                                   <  1:14461097/149‑1 (MQ=255)
                  ggcggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATAc                                 >  1:14462283/1‑150 (MQ=255)
                  ggcggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATAc                                 <  1:14459233/150‑1 (MQ=255)
                  ggcggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTAATAc                                 >  2:14462275/1‑150 (MQ=255)
                  ggcggTGCTTGTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACGGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATAc                                 <  2:14458904/150‑1 (MQ=255)
                   gcggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACt                                >  2:14462290/1‑150 (MQ=255)
                    cggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAAGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCa                                    <  1:14460375/145‑1 (MQ=255)
                     ggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttt                              <  1:14460678/150‑1 (MQ=255)
                     ggTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACtt                               >  1:14462296/1‑149 (MQ=255)
                      gTGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACtttt                             <  1:14460185/150‑1 (MQ=255)
                       tGCTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttg                            <  2:14460262/150‑1 (MQ=255)
                        gCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTCAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgt                           >  1:14462308/1‑150 (MQ=255)
                         cTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgtt                          <  1:14459722/150‑1 (MQ=255)
                         cTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgt                           <  2:14461309/149‑1 (MQ=255)
                         cTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgt                           <  2:14460963/149‑1 (MQ=255)
                         cTTTTGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGAAAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgtt                          >  2:14462309/1‑150 (MQ=255)
                          ttttGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgttt                         >  1:14462320/1‑150 (MQ=255)
                           tttGCCGTTCCGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACtttt                             <  2:14459705/145‑1 (MQ=255)
                           tttGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgtttt                        >  1:14462329/1‑150 (MQ=255)
                           tttGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgttt                         >  2:14462330/1‑149 (MQ=255)
                            ttGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttttgtttt                        <  1:14459593/149‑1 (MQ=255)
                             tGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTGATACttttgttttg                       <  1:14459448/149‑1 (MQ=255)
                             tGCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTgg                      >  1:14462339/1‑150 (MQ=255)
                              gCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGt                                            <  2:14457239/127‑1 (MQ=255)
                              gCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACttt                              <  1:14459657/141‑1 (MQ=255)
                              gCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTggg                     >  1:14462347/1‑150 (MQ=255)
                              gCCGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTgg                      <  1:14461524/149‑1 (MQ=255)
                               ccGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGt                                      <  1:14460239/132‑1 (MQ=255)
                               ccGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTgg                      <  2:14460859/148‑1 (MQ=255)
                               ccGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGgt                    <  2:14459334/150‑1 (MQ=255)
                               ccGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGgt                    <  2:14460287/150‑1 (MQ=255)
                               ccGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGgt                    <  2:14460509/150‑1 (MQ=255)
                               ccGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGgt                    <  1:14461920/150‑1 (MQ=255)
                                cGTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGgtg                   <  1:14460463/150‑1 (MQ=255)
                                 gTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGgtgt                  <  1:14460570/150‑1 (MQ=255)
                                 gTTACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGgtg                   >  2:14462353/1‑149 (MQ=255)
                                  ttACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTa                 <  1:14460599/150‑1 (MQ=255)
                                  ttACGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTa                 >  1:14462358/1‑150 (MQ=255)
                                    aCGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTAt                <  2:14460011/149‑1 (MQ=255)
                                    aCGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTCTTGTTTTGGGTGTAtt               >  1:14462370/1‑150 (MQ=255)
                                     cGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTAttt              <  1:14460994/150‑1 (MQ=255)
                                     cGCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTAt                <  2:14460336/148‑1 (MQ=255)
                                      gCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCCGTAAGTTGGCAGCATCACCCATAAATGTATAAGt                                      <  2:14459409/125‑1 (MQ=255)
                                      gCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTAttt              >  1:14462381/1‑149 (MQ=255)
                                      gCACCACCCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTAttt              >  2:14462378/1‑149 (MQ=255)
                                      gCACCACCCCCTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTgg                      <  1:14460134/141‑1 (MQ=255)
                                         ccactCCGACAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTATTTTGGgtg                   <  2:14460547/141‑1 (MQ=255)
                                         ccaccCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt          >  1:14462391/1‑150 (MQ=255)
                                          caccCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt          <  2:14460797/149‑1 (MQ=255)
                                          caccCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc         >  2:14462399/1‑150 (MQ=255)
                                          caccCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc         >  1:14462398/1‑150 (MQ=255)
                                           accCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATg                                             <  2:14459619/113‑1 (MQ=255)
                                           accCCGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATc         >  2:14462410/1‑149 (MQ=255)
                                           accCCGTCAGCAGCTGAACAGGAGGGACAGCTGACAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGtata                                         >  1:14462415/1‑117 (MQ=255)
                                             cccGTCAGCAGCTCAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAAt          <  1:14460311/146‑1 (MQ=255)
                                              ccGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGtt     <  2:14461148/150‑1 (MQ=255)
                                              ccGTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGt      >  2:14462422/1‑149 (MQ=255)
                                                gTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCGCCCATAAATGNATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa    >  1:14462435/1‑149 (MQ=255)
                                                gTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTNTAAGTCATACttttgtttt                        <  2:14460743/129‑1 (MQ=255)
                                                gTCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTa    >  2:14462431/1‑149 (MQ=255)
                                                 tCAGCAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTaaa  <  1:14458744/150‑1 (MQ=255)
                                                     |                                                                                                                                                 
GAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAA  >  NC_000913/1979123‑1979321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A