Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F13 I1 R1 15 2083.3 68373553 99.6% 68100058 141.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,361,448 (A)8→7 coding (487/1539 nt) cadC ← DNA‑binding transcriptional activator CadC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,361,4410A.97.2% 10990.4 / 284.7 2063coding (494/1539 nt)cadCDNA‑binding transcriptional activator CadC
Reads supporting (aligned to +/- strand):  ref base A (11/47);  new base . (1064/939);  total (1076/987)
Fisher's exact test for biased strand distribution p-value = 1.69e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2248 total aligned reads displayed.

CGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAG  >  NC_000913/4361293‑4361586
                                                                                                                                                    |                                                                                                                                                 
 ggggAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGaa                                                                                                                                                  >  1:32066073/1‑149 (MQ=255)
  gggAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGaaaa                                                                                                                                                <  1:32063874/150‑1 (MQ=255)
     aaCTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGaaaaaaa                                                                                                                                             <  1:32064013/150‑1 (MQ=255)
      aCTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGaaaaaaa                                                                                                                                             <  1:32064423/149‑1 (MQ=255)
       cTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAATGCTACCAGTGCTACACAGATACCTAACGACAACAGGaaaaaaac                                                                                                                                            >  2:32066105/1‑148 (MQ=255)
        tCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGaaaaaaacca                                                                                                                                          >  1:32066122/1‑147 (MQ=255)
           aGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACGCAGATACCTAACGACAACAGG‑AAAAAAACCATAc                                                                                                                                      >  1:32066154/1‑150 (MQ=255)
             cTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATAcc                                                                                                                                     >  2:32066170/1‑149 (MQ=255)
               gTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCa                                                                                                                                   >  1:32066199/1‑149 (MQ=255)
                ttACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCaa                                                                                                                                  <  2:32064146/149‑1 (MQ=255)
                ttACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCa                                                                                                                                   <  1:32064832/148‑1 (MQ=255)
                  aCAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCaaaa                                                                                                                                <  2:32064409/149‑1 (MQ=255)
                  aCAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAg                                                                                                                               >  1:32066234/1‑150 (MQ=255)
                  aCAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACACAAAAg                                                                                                                               >  2:32066240/1‑150 (MQ=255)
                   cAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCaa                                                                                                                                  <  2:32064284/147‑1 (MQ=255)
                   cAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAgg                                                                                                                              <  2:32064071/150‑1 (MQ=255)
                     aCTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAgg                                                                                                                              >  1:32066270/1‑148 (MQ=255)
                     aCTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTa                                                                                                                            <  1:32064331/150‑1 (MQ=255)
                        tttatttaCCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGt                                                                                                                          >  2:32066295/1‑149 (MQ=255)
                         ttatttaCCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTg                                                                                                                         >  1:32066307/1‑149 (MQ=255)
                           atttaCCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAAAGACAACAGG‑AAACAAACCATACCCAAAAggg                                                                                                                             >  1:32066335/1‑142 (MQ=255)
                              taCCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGt                                                                                                                   >  2:32066357/1‑150 (MQ=255)
                                ccATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGttt                                                                                                                 <  1:32064240/150‑1 (MQ=255)
                                ccATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGtt                                                                                                                  <  1:32064912/149‑1 (MQ=255)
                                 cATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTg                                                                                                                >  1:32066385/1‑150 (MQ=255)
                                   tatTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGc                                                                                                               >  2:32066412/1‑149 (MQ=255)
                                   tatTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCt                                                                                                              <  1:32064765/150‑1 (MQ=255)
                                    atTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCtt                                                                                                             >  1:32066427/1‑150 (MQ=255)
                                        aTGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTaa                                                                                                         >  1:32066471/1‑150 (MQ=255)
                                        aTGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTaa                                                                                                         <  1:32064105/150‑1 (MQ=255)
                                        aTGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAAAAAAAAAA‑AAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTaa                                                                                                         <  2:32065157/150‑1 (MQ=255)
                                        aTGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACATAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTaa                                                                                                         >  2:32066469/1‑150 (MQ=255)
                                         tGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGttt                                                                                                                 <  2:32064860/141‑1 (MQ=255)
                                            cAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTgg                                                                                                     >  2:32066511/1‑150 (MQ=255)
                                             aaTATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTggg                                                                                                    >  1:32066523/1‑150 (MQ=255)
                                               tatCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCttt                                                                                                            <  2:32064572/140‑1 (MQ=255)
                                               tatCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGa                                                                                                  <  1:32063784/150‑1 (MQ=255)
                                                atCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGtt                                                                                                                  >  1:32066565/1‑133 (MQ=255)
                                                atCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTgggg                                                                                                   >  2:32066560/1‑148 (MQ=255)
                                                   gcgTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTg                                                                                               <  1:32065714/149‑1 (MQ=255)
                                                    cgTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCtt                                                                                                             <  1:32064481/134‑1 (MQ=255)
                                                     gTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGt                                                                                              >  1:32066600/1‑148 (MQ=255)
                                                     gTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTc                                                                                            <  1:32064699/150‑1 (MQ=255)
                                                      tGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCa                                                                                           <  2:32065246/150‑1 (MQ=255)
                                                       ggAATGAACAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGaaaaaaaaaaaaaa                                                                                                                                              <  2:32063930/99‑1 (MQ=255)
                                                        gATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTGCCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAgg                                                                                         >  2:32066617/1‑150 (MQ=255)
                                                          ttGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGt                                                                                        <  2:32063792/149‑1 (MQ=255)
                                                           tGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTgg                                                                                      >  1:32066638/1‑150 (MQ=255)
                                                            gAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTgg                                                                                      <  1:32063569/149‑1 (MQ=255)
                                                             aGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGc                                                                                     <  1:32064530/149‑1 (MQ=255)
                                                             aGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCg                                                                                    <  1:32064621/150‑1 (MQ=255)
                                                              gCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCg                                                                                    >  2:32066659/1‑149 (MQ=255)
                                                                 aaaTACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTg                                                                                 <  1:32065067/149‑1 (MQ=255)
                                                                  aaTACGCGATTTGCTCATANGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCg                                                                               >  2:32066698/1‑150 (MQ=255)
                                                                   aTACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCgg                                                                              >  1:32066705/1‑150 (MQ=255)
                                                                    tACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGgt                                                                             <  2:32064938/150‑1 (MQ=255)
                                                                      cgcgATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAAATGGGGATTGTTCAGGTGGCGTTGCGgtgt                                                                           <  2:32064662/150‑1 (MQ=255)
                                                                       gcgATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGtt                                                                          >  2:32066741/1‑150 (MQ=255)
                                                                        cgATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGAaaaaaaaaaaaaaa                                                                                                                                             <  2:32063525/83‑1 (MQ=255)
                                                                        cgATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGttt                                                                         >  1:32066751/1‑150 (MQ=255)
                                                                           tttGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGtttt                                                                        >  2:32066783/1‑148 (MQ=255)
                                                                             tGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAAt                                                                    >  1:32066801/1‑150 (MQ=255)
                                                                                tCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACCGATACCTAACGACAACAGG‑AAAAAAACCATACCCaaaa                                                                                                                                >  2:32066828/1‑87 (MQ=255)
                                                                                  aTAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGtt                                                                 >  1:32066844/1‑148 (MQ=255)
                                                                                  aTAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTa                                                                <  1:32065307/149‑1 (MQ=255)
                                                                                      gAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACt                                                           >  2:32066874/1‑150 (MQ=255)
                                                                                       aaGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACt                                                           >  1:32066889/1‑149 (MQ=255)
                                                                                          aCGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTgg                                                       <  2:32065326/150‑1 (MQ=255)
                                                                                            gtgtATCAAGACTTGAAAACGCTGCCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTCGCTCTTAACTggg                                                                                                    >  2:32066919/1‑103 (MQ=255)
                                                                                            gtgtATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTggga                                                     <  2:32065075/150‑1 (MQ=255)
                                                                                            gtgtATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTggga                                                     <  1:32065203/150‑1 (MQ=255)
                                                                                              gtATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTgggagg                                                   >  1:32066933/1‑150 (MQ=255)
                                                                                                 tCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGggagggag                                                <  2:32065017/150‑1 (MQ=255)
                                                                                                    aGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAAt                                             >  2:32066963/1‑150 (MQ=255)
                                                                                                      aCTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTgggagg                                                   >  1:32066975/1‑142 (MQ=255)
                                                                                                         tGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCaaa                                                                                                                                 <  2:32064751/61‑1 (MQ=255)
                                                                                                          gAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCtgtg                                        <  1:10729996/149‑1 (MQ=255)
                                                                                                            aaaCGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTgg                                       >  1:32067013/1‑148 (MQ=255)
                                                                                                             aaCGCTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAg                                    >  2:32067018/1‑150 (MQ=255)
                                                                                                                 cTACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTgg                                                       >  2:32067056/1‑127 (MQ=255)
                                                                                                                  tACCAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAAcc                               >  1:32067068/1‑150 (MQ=255)
                                                                                                                    ncNGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTg                                                                                                      <  1:32063298/76‑1 (MQ=255)
                                                                                                                     cAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAg                                                                                                                               <  2:32062953/51‑1 (MQ=255)
                                                                                                                     cAGTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGcc                            <  1:32065142/150‑1 (MQ=255)
                                                                                                                       gTGCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAAcc                               >  2:32067101/1‑145 (MQ=255)
                                                                                                                         gCTACACAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTATTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTg                        >  1:32067115/1‑150 (MQ=255)
                                                                                                                            acacAGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCctct                         <  2:32064508/146‑1 (MQ=255)
                                                                                                                             cacaGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGtat                    <  1:32064987/150‑1 (MQ=255)
                                                                                                                               caGATACCTAACGACAACAGG‑AAAAAAACCATCCCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGtata                   <  2:32065404/149‑1 (MQ=255)
                                                                                                                               caGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAg                  <  1:32065579/150‑1 (MQ=255)
                                                                                                                               caGATACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAg                  >  2:32067142/1‑150 (MQ=255)
                                                                                                                                  aTACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAgggg               >  1:32067173/1‑150 (MQ=255)
                                                                                                                                   tACCTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTATTGAATGTTAAGACTGTGTGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAgggg               >  2:32067181/1‑149 (MQ=255)
                                                                                                                                     ccTAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGa             <  1:32065417/149‑1 (MQ=255)
                                                                                                                                       tAACGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGc          >  1:32067208/1‑150 (MQ=255)
                                                                                                                                          cGACAACAGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGaa       <  2:32066029/150‑1 (MQ=255)
                                                                                                                                           gACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGGTTGCTCTTCACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTgggaggga                                                 >  2:32067234/1‑108 (MQ=255)
                                                                                                                                            acaacaGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCg         >  1:32067246/1‑146 (MQ=255)
                                                                                                                                             caacaGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGa     <  2:32065523/149‑1 (MQ=255)
                                                                                                                                               acaGG‑AAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATa   <  2:32065746/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGGGGAACTCCAGCTGTTACAACTTTTATTTACCATATTAATGTCAATATCGCGTGGATTGAGCAAAATACGCGATTTGCTCATAGGAAGACGTGTATCAAGACTTGAAAACGCTACCAGTGCTACACAGATACCTAACGACAACAGGAAAAAAAACCATACCCAAAAGGTAGTGAATCGTTTGCTTTTAACTGGGGATTGTTCAGGTGGCGTTGCGGTGTTTTGAATGTTAAGACTGTGGGAGGGAGAATCTGTGGCAGGAACCGCCTCTGGTATAGGGGGAGGCGAAGATAG  >  NC_000913/4361293‑4361586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A