Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F13 I1 R1 17 935.6 30504812 99.2% 30260773 142.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,119,518 (T)7→6 coding (1069/1119 nt) solA ← N‑methyl‑L‑tryptophan oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,119,5120T.99.5% 4405.6 / 13.6 784coding (1075/1119 nt)solAN‑methyl‑L‑tryptophan oxidase
Reads supporting (aligned to +/- strand):  ref base T (4/0);  new base . (348/430);  total (353/431)
Fisher's exact test for biased strand distribution p-value = 4.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 847 total aligned reads displayed.

GATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAATCTTCGTCAGGCGAATTATCA  >  NC_000913/1119363‑1119657
                                                                                                                                                     |                                                                                                                                                 
gATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCt                                                                                                                                                   >  2:3634746/1‑150 (MQ=255)
    aaGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCttttt                                                                                                                                               <  1:3634072/150‑1 (MQ=255)
     aGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCtttttt                                                                                                                                              <  2:3633888/150‑1 (MQ=255)
       cATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCttttttgt                                                                                                                                            >  1:3634756/1‑148 (MQ=255)
        aTCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCttttttgtc                                                                                                                                           <  2:3634256/150‑4 (MQ=255)
         tCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCttttttgtct                                                                                                                                          >  2:3634763/1‑146 (MQ=255)
           aGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCtt                                                                                                                                        <  2:3633903/149‑1 (MQ=255)
           aGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTg                                                                                                                                       >  1:3634774/1‑150 (MQ=255)
            gATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCttttttttctt                                                                                                                                         >  2:3634778/1‑144 (MQ=255)
                   gcgAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCaaa                                                                                                                                 <  1:3634348/148‑1 (MQ=255)
                     gAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCg                                                                                                                             >  2:3634790/1‑150 (MQ=255)
                     gAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCg                                                                                                                             <  1:3634272/150‑1 (MQ=255)
                      aaTATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCgg                                                                                                                            >  1:3634795/1‑150 (MQ=255)
                      aaTATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCgg                                                                                                                            <  1:3633957/150‑1 (MQ=255)
                      aaTATTATTGATGAGATAGTGCAACAGCAGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCgg                                                                                                                            <  2:3633828/150‑1 (MQ=255)
                       atatTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGc                                                                                                                           <  1:3634052/150‑1 (MQ=255)
                          ttattGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGc                                                                                                                        >  1:3634812/1‑150 (MQ=255)
                          ttattGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGc                                                                                                                        >  2:3634814/1‑150 (MQ=255)
                           tattGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTAAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAg                                                                                                                         >  2:3634823/1‑148 (MQ=255)
                             ttGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTa                                                                                                                      <  2:3633771/149‑1 (MQ=255)
                                atgaGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTATTGTCTCGCGCAAAATCGGc                                                                                                                           >  2:3634837/1‑141 (MQ=255)
                                 tgaGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTcc                                                                                                                 >  1:3634840/1‑150 (MQ=255)
                                   agaTAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTgcg                                                                                                                                     >  2:3634850/1‑128 (MQ=255)
                                   agaTAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTcccc                                                                                                               <  2:3633540/150‑1 (MQ=255)
                                     aTAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTa                                                                                                             <  1:3634432/150‑1 (MQ=255)
                                       aGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTaa                                                                                                            >  1:3634857/1‑149 (MQ=255)
                                           cAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACt                                                                                                        <  2:3634635/149‑1 (MQ=255)
                                           cAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACt                                                                                                        >  2:3634876/1‑149 (MQ=255)
                                           cAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACt                                                                                                        >  1:3634871/1‑149 (MQ=255)
                                            aaCAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTg                                                                                                       <  2:3633976/149‑1 (MQ=255)
                                               aGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACg                                                                                                    >  1:3634888/1‑149 (MQ=255)
                                                gCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGc                                                                                                   >  2:3634894/1‑149 (MQ=255)
                                                gCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCa                                                                                                  <  1:3634026/150‑1 (MQ=255)
                                                  cGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCaaa                                                                                                <  1:3634102/150‑1 (MQ=255)
                                                  cGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCaaa                                                                                                <  1:3634126/150‑1 (MQ=255)
                                                   gACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAAt                                                                                               >  1:3634901/1‑150 (MQ=255)
                                                     cGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAAttt                                                                                             <  1:3633882/150‑1 (MQ=255)
                                                       cATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTa                                                                                            >  1:3634912/1‑149 (MQ=255)
                                                        aTTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTaaa                                                                                          <  1:3634299/150‑1 (MQ=255)
                                                        aTTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTaa                                                                                           >  2:3634916/1‑149 (MQ=255)
                                                         ttAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTaaaa                                                                                         <  1:3634395/150‑1 (MQ=255)
                                                            aaTACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCg                                                                                      <  2:3634309/150‑1 (MQ=255)
                                                            aaTACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCg                                                                                      <  2:3634093/150‑1 (MQ=255)
                                                            aaTACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCg                                                                                      >  1:3634925/1‑150 (MQ=255)
                                                             aTACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGt                                                                                     <  2:3633940/150‑1 (MQ=255)
                                                              tACTGCTGTAAAAACGGCCTCACTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTg                                                                                    <  1:3633922/150‑1 (MQ=255)
                                                                 tCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGcc                                                                                  >  2:3634940/1‑149 (MQ=255)
                                                                  ccTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCa                                                                                >  1:3634942/1‑150 (MQ=255)
                                                                   cTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGccc                                                                                 <  2:3634150/148‑1 (MQ=255)
                                                                   cTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCa                                                                                <  2:3634563/149‑1 (MQ=255)
                                                                      tAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCa                                                                            <  2:3634445/150‑1 (MQ=255)
                                                                       aaaaaCGGCCTNATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGTGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAg                                                                           >  1:3634952/1‑150 (MQ=255)
                                                                        aaaaCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAgg                                                                          <  2:3634333/150‑1 (MQ=255)
                                                                        aaaaCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAg                                                                           <  1:3634083/149‑1 (MQ=255)
                                                                         aaaCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAgg                                                                          >  2:3634965/1‑149 (MQ=255)
                                                                          aaCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGcc                                                                        <  1:3634167/150‑1 (MQ=255)
                                                                          aaCGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGcc                                                                        >  1:3634970/1‑150 (MQ=255)
                                                                              gCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGt                                                                     >  1:3634983/1‑149 (MQ=255)
                                                                               ccTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTaa                                                                   <  2:3634002/150‑1 (MQ=255)
                                                                                cTCNTTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCt                                                                                                                                                   <  1:3633738/70‑1 (MQ=255)
                                                                                cTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTaa                                                                   >  2:3634987/1‑149 (MQ=255)
                                                                                   aTTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGa                                                                >  1:3634997/1‑149 (MQ=255)
                                                                                    ttCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGc                                                              <  1:3633782/150‑1 (MQ=255)
                                                                                     tCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAg                                                               >  2:3635003/1‑148 (MQ=255)
                                                                                      cTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCa                                                             <  1:3634471/149‑1 (MQ=255)
                                                                                          aGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAgtgt                                                         >  1:3635015/1‑149 (MQ=255)
                                                                                             ccGTAATGATGATTATTGGAAGCGGGATAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGtatta                                                     <  2:3634407/150‑1 (MQ=255)
                                                                                              cGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTa                                                                    >  2:3635037/1‑134 (MQ=255)
                                                                                              cGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGtattat                                                    >  1:3635032/1‑150 (MQ=255)
                                                                                                  atgatgATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATATCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTc                                                                             >  2:3635049/1‑121 (MQ=255)
                                                                                                   tgatgaTTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAACTTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTg                                                >  1:3635051/1‑149 (MQ=255)
                                                                                                     atgatTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGc                                              <  2:3634212/149‑1 (MQ=255)
                                                                                                        attattGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCgg                                           <  1:3633999/149‑1 (MQ=255)
                                                                                                             tGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCgggtaggg                                     >  2:3635070/1‑150 (MQ=255)
                                                                                                               gAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCgggtagggta                                   >  1:3635072/1‑150 (MQ=255)
                                                                                                               gAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCgggtagggt                                    <  2:3634289/149‑1 (MQ=255)
                                                                                                                   cGGGAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCg                                <  2:3634175/149‑1 (MQ=255)
                                                                                                                    gggAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGa                               >  1:2341223/1‑149 (MQ=255)
                                                                                                                    gggAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGa                               >  1:3635085/1‑149 (MQ=255)
                                                                                                                     ggAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCa                                                                                <  2:3634243/99‑1 (MQ=255)
                                                                                                                     ggAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGata                             <  1:3633837/150‑1 (MQ=255)
                                                                                                                     ggAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGat                              <  2:3634383/149‑1 (MQ=255)
                                                                                                                      gAAAGCCTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGataa                            <  1:3634584/150‑1 (MQ=255)
                                                                                                                        aaGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTT‑TTTTTTTTTTAATCGGCAGCTATTTACCCTAAAAc                                                                                                         >  2:3635092/1‑71 (MQ=255)
                                                                                                                         aGCCTGAATGACGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGAtaataa                         <  1:3634692/150‑1 (MQ=255)
                                                                                                                            cTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAAt                      >  1:3635100/1‑150 (MQ=255)
                                                                                                                            cTGAATGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAAt                      <  2:3634034/150‑1 (MQ=255)
                                                                                                                               aaTGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCGAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGNTAATAAAATCt                    <  1:3634203/149‑1 (MQ=255)
                                                                                                                                aTGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAATTCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGcc                                             >  2:3635117/1‑123 (MQ=255)
                                                                                                                                aTGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTNCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAATCTTc                  <  1:3633593/150‑1 (MQ=255)
                                                                                                                                aTGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAATATCTTc                  <  1:3634512/150‑1 (MQ=255)
                                                                                                                                aTGGCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAATCTTc                  >  1:3635115/1‑150 (MQ=255)
                                                                                                                                   gCGTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAATCTTCGTc               <  1:3634230/150‑1 (MQ=255)
                                                                                                                                     gTCAAATCAAAATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAATCTTCGTCa              <  1:3634141/149‑1 (MQ=255)
                                                                                                                                      tcaaatcaaaATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCgggtagggta                                   <  2:3634191/127‑1 (MQ=255)
                                                                                                                                        aaatcaaaATCGCTTTTTTT‑TTTTTCTTAAAATCGGAAGCTAGTTcc                                                                                                                 >  2:3635133/1‑47 (MQ=255)
                                                                                                                                        aaatcaaaATCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAATCTTCGTCAGGc           >  1:3635130/1‑149 (MQ=255)
                                                                                                                                              aaaTCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAATCTTCGTCAGGCGAATTAt    <  2:3634499/150‑1 (MQ=255)
                                                                                                                                              aaaTCGC‑TTTTTAGTCTTGCGCAATATCGGCAGCTGTTTCCGCTAAAACTGACGCAAATTTGAAACCGTGCCCACTCAGGCCAGTAATGAGCAGTGTATTATCGTGGCCCGGTAGGGTATCGATAATAAAATCTTCGTCAGGCGAATTa     <  2:3633678/149‑1 (MQ=255)
                                                                                                                                               aaTCGC‑TTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCNTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCAGGTAGGGTATCGATAATAAAATCTTCGTCAGGCGAATTa     >  1:3635148/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GATAAAGCATCAGATGCTCGCGAATATTATTGATGAGATAGTGCAACAGCCGACGCATTAAATACTCCTGTAAAAACGGCCTCATTCTGGAGGCCGTAATGATGATTATTGGAAGCGGGAAAGCCTGAATGGCGTCAAATCAAAATCGCTTTTTTTGTCTTGCGCAAAATCGGCAGCTATTTCCCCTAAAACTGACGCAAATTTAAAACCGTGCCCACTCAGGCCGGTAATGAGCAGTGTATTATCGTGGCCGGGTAGGGTATCGATAATAAAATCTTCGTCAGGCGAATTATCA  >  NC_000913/1119363‑1119657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A