Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F13 I1 R1 17 935.6 30504812 99.2% 30260773 142.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,784,419 T→C C481C (TGT→TGC)  fadK → short chain acyl‑CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,784,4190TC99.7% 3180.9 / ‑5.2 775C481C (TGT→TGC) fadKshort chain acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (442/330);  minor base A (2/0);  total (444/331)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 784 total aligned reads displayed.

CAGCGGCGATCTCTGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATAT  >  NC_000913/1784272‑1784565
                                                                                                                                                   |                                                                                                                                                  
 aGCGGCGATCTCTGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCtgcgtg                                                                                                                                                 >  2:5520375/1‑150 (MQ=255)
 aGCGGCGATCTCTGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCtgcgtg                                                                                                                                                 <  1:5519065/150‑1 (MQ=255)
  gcggcgATCTCTGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTgg                                                                                                                                                >  1:5520378/1‑150 (MQ=255)
       gATCTCTGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGc                                                                                                                                            >  1:5520391/1‑149 (MQ=255)
         tctcTGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAACTATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAAt                                                                                                                                         >  2:5520395/1‑150 (MQ=255)
           tctGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGt                                                                                                                                       >  1:5520405/1‑150 (MQ=255)
            ctGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGc                                                                                                                                            <  2:5519584/144‑1 (MQ=255)
             tGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCa                                                                                                                                           <  1:5520044/144‑1 (MQ=255)
             tGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAAt                                                                                                                                         <  2:5519821/146‑1 (MQ=255)
              gCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGAGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTc                                                                                                                                      >  2:5520410/1‑148 (MQ=255)
               ccGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGa                                                                                                                                   <  1:5519547/150‑1 (MQ=255)
                cGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGAt                                                                                                                                  <  1:5519670/150‑1 (MQ=255)
                   aTGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGaa                                                                                                                               >  2:5520426/1‑150 (MQ=255)
                   aTGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGaa                                                                                                                               >  1:5520424/1‑150 (MQ=255)
                    tGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAAc                                                                                                                              <  1:5519900/150‑1 (MQ=255)
                     ggATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAAc                                                                                                                              <  2:5519748/149‑1 (MQ=255)
                      gATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCTAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCATGCGTGGTTGCAATGTCCGATGAACGt                                                                                                                            >  2:5520441/1‑150 (MQ=255)
                       aTGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGtt                                                                                                                           >  1:5520442/1‑150 (MQ=255)
                          aGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTa                                                                                                                         <  2:5519464/149‑1 (MQ=255)
                            gctggctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGTAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGt                                                                                                                      >  2:5520455/1‑150 (MQ=255)
                            gctggctATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGGACGTTTAGGt                                                                                                                      >  1:5520456/1‑150 (MQ=255)
                                  tatataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGa                                                                                                                >  1:5520473/1‑150 (MQ=255)
                                   atataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGAt                                                                                                               >  2:5520478/1‑150 (MQ=255)
                                    tataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGAt                                                                                                               >  1:5520485/1‑149 (MQ=255)
                                    tataAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATc                                                                                                              <  1:5519885/150‑1 (MQ=255)
                                        aaaaTTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGttt                                                                                                                          >  1:5520498/1‑134 (MQ=255)
                                        aaaaTTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATgc                                                                                                          <  2:5520023/150‑1 (MQ=255)
                                        aaaaTTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATGCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATgc                                                                                                          >  2:5520497/1‑150 (MQ=255)
                                             tACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTa                                                                                                     >  2:5520513/1‑150 (MQ=255)
                                              aCCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTAt                                                                                                    >  1:5520516/1‑150 (MQ=255)
                                                   aCGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGt                                                                                               >  1:5520529/1‑150 (MQ=255)
                                                     gCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGc                                                                                             <  1:5520010/150‑1 (MQ=255)
                                                      cAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTg                                                                                              >  2:5520537/1‑148 (MQ=255)
                                                      cAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCt                                                                                            <  2:5520140/150‑1 (MQ=255)
                                                       aaaaaaGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCt                                                                                            >  1:5520540/1‑149 (MQ=255)
                                                        aaaaaGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTg                                                                                           >  2:5520547/1‑149 (MQ=255)
                                                        aaaaaGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGa                                                                                          <  1:5519474/150‑1 (MQ=255)
                                                        aaaaaGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGa                                                                                          <  2:5519537/150‑1 (MQ=255)
                                                           aaGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCATCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAg                                                                                       >  1:5520558/1‑150 (MQ=255)
                                                             gATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcg                                                                                     <  1:5519806/150‑1 (MQ=255)
                                                              atatTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcgc                                                                                    >  2:5520567/1‑150 (MQ=255)
                                                                 ttattGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGcgccg                                                                                  >  1:5520574/1‑149 (MQ=255)
                                                                 ttattGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGcgccg                                                                                  <  1:5519867/149‑1 (MQ=255)
                                                                   attGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGTTGAACGATCATgcg                                                                                                         >  2:5520585/1‑124 (MQ=255)
                                                                   attGGCCGCGGCGGCGGAAAGATGAGCATCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGc                                                                                                                                            <  2:5519943/89‑1 (MQ=255)
                                                                    ttGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGcat                                                                               >  1:5520588/1‑149 (MQ=255)
                                                                     tGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGACCGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgc                                                                                      <  2:5519275/141‑1 (MQ=255)
                                                                      gTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGcatcat                                                                            >  1:5520601/1‑150 (MQ=255)
                                                                       tCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCAtcatt                                                                           >  2:5520605/1‑150 (MQ=255)
                                                                         cgcgGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGa                                                                                          <  2:5519638/133‑1 (MQ=255)
                                                                          gcggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCAtcattcat                                                                        <  2:5519974/150‑1 (MQ=255)
                                                                           cggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCAtcattcat                                                                        <  2:5520050/149‑1 (MQ=255)
                                                                           cggcggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCAtcattcat                                                                        >  1:5520615/1‑149 (MQ=255)
                                                                              cggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATc                                                                    <  1:5520088/150‑1 (MQ=255)
                                                                              cggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATc                                                                    >  1:5520624/1‑150 (MQ=255)
                                                                               ggcgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCGAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCtt                                                                                                      <  2:5519685/115‑1 (MQ=255)
                                                                                 cgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCt                                                                 >  2:5520628/1‑150 (MQ=255)
                                                                                 cgAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCt                                                                 <  1:5519739/150‑1 (MQ=255)
                                                                                   aaaaTATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTg                                                                >  1:5520641/1‑149 (MQ=255)
                                                                                   aaaaTATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTg                                                                <  2:5519914/149‑1 (MQ=255)
                                                                                     aaTATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTgg                                                               >  2:5520644/1‑148 (MQ=255)
                                                                                         ttAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAagag                                                          >  1:5520655/1‑149 (MQ=255)
                                                                                           agcagcCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAgg                                                         >  2:5520659/1‑148 (MQ=255)
                                                                                            gcagcCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTa                                                       <  1:5519933/149‑1 (MQ=255)
                                                                                              agcCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTg                                                    >  1:5520669/1‑150 (MQ=255)
                                                                                               gcCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGNGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTg                                                    <  1:5520160/149‑1 (MQ=255)
                                                                                                ccGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACCATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGcgcc                                                                                   >  2:5520680/1‑117 (MQ=255)
                                                                                                ccGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAACTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTgg                                                   <  1:5519305/149‑1 (MQ=255)
                                                                                                 cGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAgcgc                                                                                    >  2:5520691/1‑115 (MQ=255)
                                                                                                 cGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCt                                                 >  1:5520687/1‑150 (MQ=255)
                                                                                                   tGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCttt                                               <  1:5519975/150‑1 (MQ=255)
                                                                                                     aaGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCttttt                                             <  1:5519949/150‑1 (MQ=255)
                                                                                                     aaGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCttttt                                             >  1:5520702/1‑150 (MQ=255)
                                                                                                          gAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTACGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGATGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGc                                        >  2:5520711/1‑150 (MQ=255)
                                                                                                           aaGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGAGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTgg                                                   >  1:5520716/1‑138 (MQ=255)
                                                                                                               tatTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTaa                                   <  2:5520003/150‑1 (MQ=255)
                                                                                                                atTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTaaa                                  >  1:5520726/1‑150 (MQ=255)
                                                                                                                atTTTATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTaa                                   >  2:5520730/1‑149 (MQ=255)
                                                                                                                  tttATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTaaa                                  <  2:5520066/148‑1 (MQ=255)
                                                                                                                  tttATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAAc                                 <  1:5519632/149‑1 (MQ=255)
                                                                                                                   ttATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACgg                               >  1:5520739/1‑150 (MQ=255)
                                                                                                                   ttATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACg                                <  2:5519781/149‑1 (MQ=255)
                                                                                                                    tATTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCt                                                                 >  2:5520749/1‑115 (MQ=255)
                                                                                                                     aTTGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACggg                              <  1:5520250/149‑1 (MQ=255)
                                                                                                                      ttGCAGCATCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGt                             <  2:5520113/149‑1 (MQ=255)
                                                                                                                        gcagcaTCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCg                           <  2:5520294/149‑1 (MQ=255)
                                                                                                                        gcagcaTCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCg                           >  1:5520757/1‑149 (MQ=255)
                                                                                                                         cagcagCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTGCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAACGCGCCGCATCATTCATTATCGCTGGAAGAGGGAGTGGCTTTTTTTAGCCGTAAACGGGTCGc                          <  1:5520135/149‑1 (MQ=255)
                                                                                                                         cagcaTCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGc                          >  2:5520762/1‑149 (MQ=255)
                                                                                                                            caTCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCaaaa                      >  1:5520773/1‑150 (MQ=255)
                                                                                                                             aTCCTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCaaaa                      >  2:5520776/1‑149 (MQ=255)
                                                                                                                                cTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatata                  <  2:5519860/150‑1 (MQ=255)
                                                                                                                                cTAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaata                    >  2:5520786/1‑148 (MQ=255)
                                                                                                                                 tAAAATTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatata                  >  1:5520792/1‑149 (MQ=255)
                                                                                                                                     aTTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatataaatat             >  1:5520806/1‑150 (MQ=255)
                                                                                                                                     aTTCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAaaatataaata              >  2:5520808/1‑149 (MQ=255)
                                                                                                                                       tCACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATcc           >  2:5520817/1‑150 (MQ=255)
                                                                                                                                        cACGATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATcc           <  1:5519586/149‑1 (MQ=255)
                                                                                                                                           gATGCCTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGa        <  2:5520192/149‑1 (MQ=255)
                                                                                                                                               ccTGCGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACata   >  1:5520830/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CAGCGGCGATCTCTGCCGTATGGATGAGGCTGGCTATATAAAAATTACCGGACGCAAAAAAGATATTATTGTCCGCGGCGGCGAAAATATTAGCAGCCGTGAAGTGGAAGATATTTTATTGCAGCATCCTAAAATTCACGATGCCTGTGTGGTTGCAATGTCCGATGAACGTTTAGGTGAACGATCATGCGCTTATGTCGTGCTGAAAGCGCCGCATCATTCATTATCGCTGGAAGAGGTAGTGGCTTTTTTTAGCCGTAAACGGGTCGCAAAATATAAATATCCTGAACATAT  >  NC_000913/1784272‑1784565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A