Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F13 I1 R1 17 935.6 30504812 99.2% 30260773 142.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,319,321 G→A Q186* (CAA→TAA)  rcsC ← sensory histidine kinase RcsC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,319,3210GA99.8% 3823.9 / ‑4.5 901Q186* (CAA→TAA) rcsCsensory histidine kinase RcsC
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (464/434);  total (466/435)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 927 total aligned reads displayed.

AAAAGTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCG  >  NC_000913/2319174‑2319464
                                                                                                                                                   |                                                                                                                                               
aaaaGTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTtatg                                                                                                                                               <  1:7108448/150‑1 (MQ=255)
 aaaGTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTatgg                                                                                                                                              <  2:7108608/150‑1 (MQ=255)
    gTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGc                                                                                                                                           <  1:7108574/150‑1 (MQ=255)
      tctcCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCAt                                                                                                                                         >  1:7109205/1‑150 (MQ=255)
      tctcCATCCGGATGGTCTGCTCGAAACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCGATCATCTTatggt                                                                                                                                             >  2:7109206/1‑146 (MQ=255)
          cATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCg                                                                                                                                     <  2:7108022/150‑1 (MQ=255)
             ccGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGata                                                                                                                                  >  1:7109223/1‑150 (MQ=255)
             ccGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGata                                                                                                                                  >  2:7109225/1‑150 (MQ=255)
                gATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGAtatt                                                                                                                                >  2:7109241/1‑149 (MQ=255)
                 aTGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGAtattta                                                                                                                              >  1:7109243/1‑150 (MQ=255)
                  tGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGAtatttat                                                                                                                             <  1:7108140/150‑1 (MQ=255)
                   ggTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGAtatttatt                                                                                                                            >  1:7109252/1‑150 (MQ=255)
                           cGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGa                                                                                                                                    <  2:7108457/134‑1 (MQ=255)
                           cGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGt                                                                                                                     >  2:7109272/1‑149 (MQ=255)
                             acacCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTc                                                                                                                   <  1:7108279/149‑1 (MQ=255)
                              cacCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTAATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCAt                                                                                                                 <  2:7108347/150‑1 (MQ=255)
                                  cAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCAt                                                                                                                 >  1:7109291/1‑146 (MQ=255)
                                   aaaaGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGtt                                                                                                                    >  2:7109295/1‑142 (MQ=255)
                                        cgcCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCttt                                                                                                       >  1:7109313/1‑150 (MQ=255)
                                        cgcCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCttt                                                                                                       >  2:7109312/1‑150 (MQ=255)
                                        cgcCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCttt                                                                                                       <  1:7108011/150‑1 (MQ=255)
                                        cgcCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCtt                                                                                                        <  2:7108490/149‑1 (MQ=255)
                                         gcCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTc                                                                                                      <  2:7108565/150‑1 (MQ=255)
                                           cTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCaa                                                                                                    >  2:7109323/1‑150 (MQ=255)
                                           cTGCAACCGGTCCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGc                                                                                                          <  2:7108206/144‑1 (MQ=255)
                                            tGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGAtatttatt                                                                                                                            <  2:7108316/125‑1 (MQ=255)
                                             gCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCg                                                                                                                                     <  2:7108701/115‑1 (MQ=255)
                                              cAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAAcgc                                                                                                 >  2:7109341/1‑150 (MQ=255)
                                              cAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAAcgc                                                                                                 >  1:7109336/1‑150 (MQ=255)
                                              cAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGACGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAAcg                                                                                                  <  1:7108404/149‑1 (MQ=255)
                                                  cGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTa                                                                                             <  2:7108585/150‑1 (MQ=255)
                                                   tgTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTAt                                                                                            >  1:7109359/2‑150 (MQ=255)
                                                   ggTTCGCCAGATAAACTGGCGTCAACGCGAAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTTCATTTCGATATTTATTGATGCGTGCATGCAAAGCTTTCAACTCGGTAt                                                                                            >  2:7109358/1‑150 (MQ=255)
                                                       cGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATcgt                                                                                         <  1:7108366/149‑2 (MQ=255)
                                                        gCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGt                                                                                       >  1:7109372/1‑150 (MQ=255)
                                                           aGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTc                                                                                                                                      >  2:7109387/1‑100 (MQ=255)
                                                             aTAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCAc                                                                                  >  1:7109391/1‑150 (MQ=255)
                                                                 aCTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCttt                                                                                                       <  2:7108136/125‑1 (MQ=255)
                                                                 aCTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAAcgc                                                                                                 >  1:7109408/1‑131 (MQ=255)
                                                                 aCTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTgg                                                                               >  2:7109406/1‑149 (MQ=255)
                                                                  cTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCa                                                                             <  2:7108671/150‑1 (MQ=255)
                                                                     gCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCat                                                                            <  1:7109104/148‑1 (MQ=255)
                                                                      cGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCNCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCatat                                                                          >  1:7109422/1‑149 (MQ=255)
                                                                        tCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCa                                                                        >  2:7109432/1‑149 (MQ=255)
                                                                        tCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCAc                                                                       <  1:7108189/150‑1 (MQ=255)
                                                                         cAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACg                                                                      >  1:7109438/1‑150 (MQ=255)
                                                                          aaCGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACg                                                                      <  2:7108412/149‑1 (MQ=255)
                                                                          aaCGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGc                                                                     <  1:7108996/150‑1 (MQ=255)
                                                                           aCGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCa                                                                    <  2:7108951/150‑1 (MQ=255)
                                                                           aCGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCa                                                                    <  1:7108540/150‑1 (MQ=255)
                                                                             gcgTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGa                                                                  <  2:7108721/150‑1 (MQ=255)
                                                                             gcgTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGa                                                                  <  1:7108722/150‑1 (MQ=255)
                                                                              cgTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGAc                                                                 >  2:7109455/1‑150 (MQ=255)
                                                                              cgTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGAc                                                                 >  1:7109454/1‑150 (MQ=255)
                                                                               gTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGAcc                                                                <  1:7108828/150‑1 (MQ=255)
                                                                                  aaaaTACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGaa                                                             <  1:7107378/150‑1 (MQ=255)
                                                                                    aaTACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCatat                                                                          >  2:7109475/1‑135 (MQ=255)
                                                                                    aaTACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt                                                           >  1:7109472/1‑150 (MQ=255)
                                                                                        cccGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGc                                                       >  2:7109492/1‑150 (MQ=255)
                                                                                        cccGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGc                                                       >  1:7109493/1‑150 (MQ=255)
                                                                                              gCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg                                                   >  2:7109515/1‑148 (MQ=255)
                                                                                              gCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg                                                   >  1:7109510/1‑148 (MQ=255)
                                                                                              gCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATg                                                   <  1:7108679/148‑1 (MQ=255)
                                                                                               ccAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGcc                                                      <  2:7108278/144‑1 (MQ=255)
                                                                                                cAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCaga                                               <  1:7108320/150‑1 (MQ=255)
                                                                                                 aGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCagag                                              >  2:7109528/1‑150 (MQ=255)
                                                                                                   ggCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATc                                                                                           <  2:7108632/103‑1 (MQ=255)
                                                                                                   ggCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATAATTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGt                                            >  1:7109536/1‑150 (MQ=255)
                                                                                                     cgcgGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAt                                                           <  2:7108900/133‑1 (MQ=255)
                                                                                                       cgGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATc                                        >  2:7109547/1‑150 (MQ=255)
                                                                                                        ggACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATc                                        <  1:7108927/149‑1 (MQ=255)
                                                                                                         gACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGc                                      >  1:7109551/1‑150 (MQ=255)
                                                                                                         gACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGc                                      <  1:7108607/150‑1 (MQ=255)
                                                                                                         gACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGACGTTATCGc                                      <  1:7108765/150‑1 (MQ=255)
                                                                                                          cccTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGtt                                           <  2:7108839/143‑1 (MQ=255)
                                                                                                          aCCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGAAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCt                                     <  2:7108744/150‑1 (MQ=255)
                                                                                                               cGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCg                                 <  1:7108856/149‑1 (MQ=255)
                                                                                                               cGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGa                                >  2:7109563/1‑150 (MQ=255)
                                                                                                                 cTGATCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGAt                               >  2:7109573/1‑149 (MQ=255)
                                                                                                                    aTCCAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTaa                            >  1:7109579/1‑149 (MQ=255)
                                                                                                                       cAGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAAt                        <  1:7108802/150‑1 (MQ=255)
                                                                                                                        aGTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATa                       >  2:7109591/1‑150 (MQ=255)
                                                                                                                         gTAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCGAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAGAAATAc                      >  1:7109597/1‑150 (MQ=255)
                                                                                                                          tAGAGGTTACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATAcc                     <  1:7108656/150‑1 (MQ=255)
                                                                                                                                 tACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGtt                >  1:7109612/1‑148 (MQ=255)
                                                                                                                                 tACTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAAATGGCCATGCAGAGGTTATCGCTGcc                                  >  2:7109615/1‑130 (MQ=255)
                                                                                                                                  aCTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTa              <  2:7109091/149‑1 (MQ=255)
                                                                                                                                   cTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGtt                >  2:7109627/1‑146 (MQ=255)
                                                                                                                                   cTGCCGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGa            <  1:7108894/150‑1 (MQ=255)
                                                                                                                                      ccGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCg         >  1:7109640/1‑150 (MQ=255)
                                                                                                                                       cGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCg         <  2:7108650/149‑1 (MQ=255)
                                                                                                                                       cGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCg         >  2:7109644/1‑149 (MQ=255)
                                                                                                                                       cGCTATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGt        <  1:7108494/150‑1 (MQ=255)
                                                                                                                                        gCTATCATCTCATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGt        >  1:7109652/1‑149 (MQ=255)
                                                                                                                                          tATCATCTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAg     >  1:7109667/1‑150 (MQ=255)
                                                                                                                                           atcatcTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGc    >  2:7109671/1‑150 (MQ=255)
                                                                                                                                             catcTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGttt               <  2:7108526/137‑1 (MQ=255)
                                                                                                                                              atcTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCNGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCg  <  1:7109269/149‑1 (MQ=255)
                                                                                                                                              atcTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCg  <  2:7109040/149‑1 (MQ=255)
                                                                                                                                              atcTTATGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCg  <  2:7108785/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
AAAAGTTCTCCATCCGGATGGTCTGCTCGACACCCAAAAGCGCCTGCAACCGGTTCGCCAGATAAACTGGCGTCAACGCGTAAAAATACCCGACGCCAGGGCGCGGACCTTCGCTGATCCAGTAGAGGTTACTGCCGCTATCATCTTGTGGTGCATTTCGATATTTATTGATGCGTTCATGCAAAGCTTTCAACGCGGTATCGCGTTCCACTGGCATATCACGCAGACCGAAATTGGCCATGCAGAGGTTATCGCTGCCGATTAAAAATACCCGGTTTAGATCGTAAGCCG  >  NC_000913/2319174‑2319464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A