Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F13 I1 R1 20 580.9 19806870 98.8% 19569187 140.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,718,041 G→A T163I (ACA→ATA)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,718,0410GA99.0% 2154.9 / 4.1 502T163I (ACA→ATA) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/5);  new base A (227/268);  total (229/273)
Fisher's exact test for biased strand distribution p-value = 6.65e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01

Only 100 of 502 total aligned reads displayed.

CCAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTGTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCAT  >  NC_000913/2717895‑2718188
                                                                                                                                                  |                                                                                                                                                   
 cAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCaa                                                                                                                                                 <  1:5584833/150‑1 (MQ=255)
    gTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTc                                                                                                                                              <  1:5583599/150‑1 (MQ=255)
    gTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCGGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAAtt                                                                                                                                               <  1:5584274/149‑1 (MQ=255)
     ttATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTcc                                                                                                                                             >  1:5585071/1‑150 (MQ=255)
     ttATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTcc                                                                                                                                             <  1:5584636/150‑1 (MQ=255)
      tATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCg                                                                                                                                            >  2:5585071/1‑150 (MQ=255)
        tGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCt                                                                                                                                          <  1:5584446/150‑1 (MQ=255)
          cGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCtt                                                                                                                                         <  2:5584820/149‑1 (MQ=255)
          cGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTc                                                                                                                                        <  1:5584579/150‑1 (MQ=255)
             gTTAGACTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATc                                                                                                                                     <  2:5584487/150‑1 (MQ=255)
              ttAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATc                                                                                                                                     >  1:5585083/1‑149 (MQ=255)
               tAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATcga                                                                                                                                   >  2:5585088/1‑148 (MQ=255)
                   ttCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATccacca                                                                                                                                >  2:5585096/1‑149 (MQ=255)
                     cGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACAt                                                                                                                             >  1:5585101/1‑150 (MQ=255)
                      gTTTTCCATTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACAt                                                                                                                             <  1:5584204/149‑1 (MQ=255)
                        tttCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATgg                                                                                                                           >  2:5585108/1‑149 (MQ=255)
                         ttCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGt                                                                                                                          <  1:5584675/149‑1 (MQ=255)
                          tCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTAc                                                                                                                        >  1:5585113/1‑150 (MQ=255)
                           ccAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTAc                                                                                                                        <  1:5584331/149‑1 (MQ=255)
                           ccAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTAc                                                                                                                        <  2:5584414/149‑1 (MQ=255)
                             aGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCt                                                                                                                     >  1:5585120/1‑150 (MQ=255)
                               tGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCttt                                                                                                                   <  1:5584537/150‑1 (MQ=255)
                               tGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCttt                                                                                                                   <  2:5584624/150‑1 (MQ=255)
                                   aGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCg                                                                                                                >  2:5585127/1‑149 (MQ=255)
                                   aGAACGCAATCCTCCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCgg                                                                                                               >  1:5585128/1‑150 (MQ=255)
                                    gAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTNCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGt                                                                                                              <  2:5584606/150‑1 (MQ=255)
                                         cAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTtgct                                                                                                         <  1:5584380/150‑1 (MQ=255)
                                          aaTCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTc                                                                                                                                        >  2:5585138/1‑118 (MQ=255)
                                            tCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTtgctgc                                                                                                       >  1:5585142/1‑149 (MQ=255)
                                              cTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCAt                                                                                                    <  1:5584658/150‑1 (MQ=255)
                                               ggcgcgcCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCAt                                                                                                    <  1:5584712/148‑1 (MQ=255)
                                                  gcgcCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATc                                                                                                   <  2:5584635/147‑1 (MQ=255)
                                                   cgcCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAgc                                                                                               >  1:5585153/1‑150 (MQ=255)
                                                   cgcCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAgc                                                                                               >  2:5585150/1‑150 (MQ=255)
                                                      ctgcctgTCTTCCCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCt                                                                                                                                          <  2:5584354/104‑1 (MQ=255)
                                                      ctgcctgGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAgcgc                                                                                             >  1:5585161/1‑149 (MQ=255)
                                                         cctgGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCaaa                                                                                          <  1:5584748/149‑1 (MQ=255)
                                                         cctgGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCaa                                                                                           <  1:5584554/148‑1 (MQ=255)
                                                         cctgGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAg                                                                                         <  2:5584854/150‑1 (MQ=255)
                                                          ctgGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCgggt                                                                                                             >  2:5585164/1‑127 (MQ=255)
                                                               ttACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAgcg                                                                                              >  2:5585174/1‑139 (MQ=255)
                                                                 aCCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCttt                                                                                  >  1:5585178/1‑149 (MQ=255)
                                                                  cccACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCttt                                                                                  <  2:5584681/148‑1 (MQ=255)
                                                                   ccACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTa                                                                                 <  1:5584847/148‑1 (MQ=255)
                                                                    cACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAgc                                                                                               >  2:5585183/1‑133 (MQ=255)
                                                                        gctgcACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAAcgcg                                                                          >  1:5585190/1‑150 (MQ=255)
                                                                           gcACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACgcggcg                                                                       >  1:5585198/1‑150 (MQ=255)
                                                                             aCGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGtt                                                                     <  1:5584800/150‑1 (MQ=255)
                                                                              cGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGctt                                                                                      >  2:5585200/1‑132 (MQ=255)
                                                                               ggTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTa                                                                    <  2:5584896/149‑1 (MQ=255)
                                                                                 ttGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTacac                                                                 <  2:5584951/150‑1 (MQ=255)
                                                                                    tACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACActct                                                              <  1:5584766/150‑1 (MQ=255)
                                                                                    tACCGTTACGCCTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCtt                                                                                                                    <  2:5584264/96‑1 (MQ=255)
                                                                                       cGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGa                                                           <  1:5584396/150‑1 (MQ=255)
                                                                                             gCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCNATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAAc                                                      >  1:5585212/1‑149 (MQ=255)
                                                                                              cTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCa                                                    <  1:5584737/150‑1 (MQ=255)
                                                                                              cTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCa                                                    <  1:5585057/150‑1 (MQ=255)
                                                                                              cTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATACAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAAc                                                      >  2:5585211/1‑148 (MQ=255)
                                                                                               tttttGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCa                                                    <  2:5584718/149‑1 (MQ=255)
                                                                                                 tttGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAgg                                                  <  1:5584485/149‑1 (MQ=255)
                                                                                                    gATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGgcgc                                               >  1:5585221/1‑149 (MQ=255)
                                                                                                    gATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGgcgc                                               <  2:5584813/149‑1 (MQ=255)
                                                                                                     aTCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGgcgc                                               >  2:5585222/1‑148 (MQ=255)
                                                                                                      tCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGcgcg                                              >  2:5585229/1‑148 (MQ=255)
                                                                                                       cAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTGTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGcgcg                                              <  1:5584689/147‑1 (MQ=255)
                                                                                                        cagacgCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCaa                                                                                                                                                 <  2:5584701/46‑1 (MQ=255)
                                                                                                        aagaagCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAAc                                           >  1:5585235/1‑149 (MQ=255)
                                                                                                         agaagCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACaa                                         <  2:5585002/150‑1 (MQ=255)
                                                                                                           aagCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCCCTTCCTCCACCACATGGTACg                                                                                                                       >  2:5585239/1‑70 (MQ=255)
                                                                                                            agCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCCCAGctg                                                                                      >  2:5585245/1‑101 (MQ=255)
                                                                                                            agCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCg                                      <  1:5584908/150‑1 (MQ=255)
                                                                                                              cAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCACAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGCACAATCGCt                                    >  1:5585248/1‑150 (MQ=255)
                                                                                                               aaaCGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCtt                                   >  1:5585256/1‑150 (MQ=255)
                                                                                                               aaaCGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCtt                                   <  1:5584936/150‑1 (MQ=255)
                                                                                                                 aCGCCGCCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTcc                                 <  1:5584597/150‑1 (MQ=255)
                                                                                                                  cgccgcCGTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGa                                                           >  2:5585259/1‑123 (MQ=255)
                                                                                                                   gccgccGTGATGTTCGGTGCCTGACGCTTTTATCATTTACGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCg                                <  2:5584653/149‑1 (MQ=255)
                                                                                                                    ccgccgTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCAGCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCg                                >  1:5585266/1‑148 (MQ=255)
                                                                                                                    ccgccgTGATGTTCCGTGCATGACGCTGTTATCAATTCCGCTTCATCCACCACATCGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGa                              <  2:5584516/150‑1 (MQ=255)
                                                                                                                     cgccgTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGGACAATcc                                      >  2:5585268/1‑140 (MQ=255)
                                                                                                                     cgccgTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGAc                             <  1:5584868/150‑1 (MQ=255)
                                                                                                                        cgTGATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACgg                           <  2:5584769/149‑1 (MQ=255)
                                                                                                                         gTGATGTTCCGTGCCTGACGCTTTTCTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAgcg                                                                                              >  2:5585275/1‑81 (MQ=255)
                                                                                                                          tggtGTTCCGTGCCTGACGCTGTTATCAATTCCGCTTCATCCACCACATGGTACGCGTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACgg                           <  1:5584728/144‑1 (MQ=255)
                                                                                                                           gATGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGctct                       <  2:5584585/150‑1 (MQ=255)
                                                                                                                            aTGTTCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTg                      >  1:5585282/1‑150 (MQ=255)
                                                                                                                               ttCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCCCATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGa                    >  2:5585282/1‑149 (MQ=255)
                                                                                                                               ttCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGaa                   <  1:5584889/150‑1 (MQ=255)
                                                                                                                               ttCCGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGa                    <  1:5584968/149‑1 (MQ=255)
                                                                                                                                 ccGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGcgcg                                              <  2:5584460/121‑1 (MQ=255)
                                                                                                                                 ccGTGCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGaaa                  <  2:5584743/149‑1 (MQ=255)
                                                                                                                                     gCCTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGc             >  1:5585292/1‑150 (MQ=255)
                                                                                                                                       cTGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCt           >  2:5585294/1‑150 (MQ=255)
                                                                                                                                        tGACGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTg                      >  2:5585300/1‑138 (MQ=255)
                                                                                                                                          aCGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGAc        <  1:5584503/150‑1 (MQ=255)
                                                                                                                                           cGCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTg                      >  2:5585306/1‑135 (MQ=255)
                                                                                                                                            gCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACa       <  1:5584825/149‑1 (MQ=255)
                                                                                                                                            gCTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACAt      >  1:5585311/1‑150 (MQ=255)
                                                                                                                                             cTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATg     <  2:5584796/150‑1 (MQ=255)
                                                                                                                                             cTTTTATCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATg     >  2:5585312/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCAGGTTATGCGGGTTAGATTCGTTTTCCAGTGCCAGAACGCAATCCTGCGCGCCTGCCTGGCTTACCCACTGCTGCACGGTTGTACCGTTACGCTTTTTGATCAAGAAGCAAACGCCGCCGTGATGTTCCGTGCCTGACGCTTTTGTCAATTCCGCTTCATCCACCACATGGTACGCTTTGCGGTTTGCTGCCATCCAGCGCAAAGCTTCTTTAAAACGCGGCGTTACACTCTGGATAAACCAGGCGCGAACAATCGCTTCCGGACGGCTCTGGAAAAGTGCCTGACATGCAT  >  NC_000913/2717895‑2718188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A