Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F13 I1 R1 116 362.0 17214306 99.7% 17162663 98.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,867,926 (C)6→7 coding (828/1140 nt) nlpD ← murein hydrolase activator NlpD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,867,9201.C99.3% 1217.2 / ‑0.7 295coding (834/1140 nt)nlpDmurein hydrolase activator NlpD
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (136/157);  total (138/157)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 351 total aligned reads displayed.

TACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGT  >  NC_000913/2867820‑2868015
                                                                                                     |                                                                                               
 aCCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑c                                                                                                <  2:5173875/101‑1 (MQ=255)
  ccGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑cc                                                                                               <  1:5173857/101‑1 (MQ=255)
  ccGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑cc                                                                                               >  1:5174280/1‑101 (MQ=255)
   cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑ccc                                                                                              >  2:5174281/1‑101 (MQ=255)
   cGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑ccc                                                                                              <  2:5173672/101‑1 (MQ=255)
    gTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑cccc                                                                                             <  2:5173591/101‑1 (MQ=255)
      aGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑cccccc                                                                                           >  2:5174286/1‑101 (MQ=255)
      aGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑cccccc                                                                                           <  1:5173737/101‑1 (MQ=255)
      aGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑cccccc                                                                                           <  2:5173545/101‑1 (MQ=255)
        ccGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑ccccccct                                                                                         >  1:5174290/1‑99 (MQ=255)
         cgcgCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑ccccccctc                                                                                        <  2:5173743/101‑4 (MQ=255)
          gcgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑ccccccctca                                                                                       >  2:5174296/1‑97 (MQ=255)
          gcgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑ccccccctca                                                                                       <  1:5173835/101‑5 (MQ=255)
           cgcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑ccccccctcag                                                                                      >  1:5174298/1‑96 (MQ=255)
            gcAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑ccccccctc                                                                                        >  2:5174302/1‑95 (MQ=255)
              aGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAg                                                                                    <  2:5173618/101‑1 (MQ=255)
              aGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAg                                                                                    <  2:5174222/101‑1 (MQ=255)
                cgcgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAgcg                                                                                  >  1:5174308/1‑101 (MQ=255)
                 gcgTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAgcgc                                                                                 >  2:5174310/1‑101 (MQ=255)
                   gTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCa                                                                               >  1:5174313/1‑101 (MQ=255)
                    ttACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCaa                                                                              <  1:5173965/101‑1 (MQ=255)
                    ttACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCaa                                                                              >  2:5174319/1‑101 (MQ=255)
                     tACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCaaa                                                                             <  1:5173632/101‑1 (MQ=255)
                     tACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCaaa                                                                             >  1:5174320/1‑101 (MQ=255)
                     tACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCaaa                                                                             <  1:5173949/101‑1 (MQ=255)
                       ccAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAgg                                                                           <  1:5173926/101‑1 (MQ=255)
                        cAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGt                                                                          <  2:5173848/101‑1 (MQ=255)
                         aGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGtt                                                                         <  2:5173963/101‑1 (MQ=255)
                         aGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGtt                                                                         >  2:5174325/1‑101 (MQ=255)
                         aGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGtt                                                                         >  1:5174326/1‑101 (MQ=255)
                          gCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGttt                                                                        <  2:5173984/101‑1 (MQ=255)
                           cATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTc                                                                       >  1:5174331/1‑101 (MQ=255)
                              aaaCAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGAt                                                                    <  2:5173883/101‑1 (MQ=255)
                              aaaCAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGAt                                                                    <  2:5173903/101‑1 (MQ=255)
                              aaaCAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGAt                                                                    >  2:5174335/1‑101 (MQ=255)
                               aacaacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATc                                                                   >  2:5174339/1‑101 (MQ=255)
                                 caacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCAc                                                                 >  1:5174340/1‑101 (MQ=255)
                                  aacGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACt                                                                >  2:5174346/1‑101 (MQ=255)
                                   acGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACtt                                                               <  1:5173714/101‑1 (MQ=255)
                                    cgcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACttt                                                              <  1:5173840/101‑1 (MQ=255)
                                    cgcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACttt                                                              <  2:5173820/101‑1 (MQ=255)
                                    cgcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACttt                                                              >  1:5174350/1‑101 (MQ=255)
                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTg                                                             >  2:5174352/1‑101 (MQ=255)
                                     gcgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTg                                                             <  2:5173787/101‑1 (MQ=255)
                                      cgGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGc                                                            <  1:5173801/101‑1 (MQ=255)
                                       ggCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGcc                                                           >  2:5174357/1‑101 (MQ=255)
                                           aTCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCa                                                       >  1:5174362/1‑101 (MQ=255)
                                            tCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAg                                                      <  1:5173727/101‑1 (MQ=255)
                                            tCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAg                                                      <  1:5174043/101‑1 (MQ=255)
                                              tGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTc                                                    <  2:5173755/101‑1 (MQ=255)
                                                cGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCgg                                                  >  2:5174365/1‑101 (MQ=255)
                                                cGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCgg                                                  <  1:5173468/101‑1 (MQ=255)
                                                  gTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGcc                                                >  1:5174368/1‑101 (MQ=255)
                                                   tCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCa                                               >  1:5174372/1‑101 (MQ=255)
                                                   tCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCa                                               <  2:5173703/101‑1 (MQ=255)
                                                     gcgATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgc                                             <  1:5173770/101‑1 (MQ=255)
                                                      cgATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAg                                              >  1:5174377/1‑99 (MQ=255)
                                                       gATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAgcgc                                           >  1:5174381/1‑101 (MQ=255)
                                                        aTAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGcc                                          >  1:5174385/1‑101 (MQ=255)
                                                        aTAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑cc                                                                                               >  2:5174386/1‑47 (MQ=255)
                                                         tAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCa                                         <  1:5173886/101‑1 (MQ=255)
                                                          aaTTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAg                                        <  1:5173988/101‑1 (MQ=255)
                                                            ttGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGt                                      >  2:5174390/1‑101 (MQ=255)
                                                            ttGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGt                                      >  1:5174392/1‑101 (MQ=255)
                                                              gCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTgg                                    <  2:5174030/101‑1 (MQ=255)
                                                                cTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgag                                  <  1:5173920/101‑1 (MQ=255)
                                                                cTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgag                                  >  2:5174398/1‑101 (MQ=255)
                                                                 tGTCCTTTGCTGTCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgaga                                 <  2:5173767/101‑1 (MQ=255)
                                                                 tGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGgaga                                 <  1:5173943/101‑1 (MQ=255)
                                                                    ccTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                              >  1:5174401/1‑101 (MQ=255)
                                                                    ccTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                              <  2:5174086/101‑1 (MQ=255)
                                                                    ccTTTGCTGCCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                              <  1:5173894/101‑1 (MQ=255)
                                                                        tgctgcCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGt                          <  1:5173864/101‑1 (MQ=255)
                                                                         gctgcCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAg                              >  1:5174406/1‑96 (MQ=255)
                                                                         gctgcCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTa                         <  2:5173811/101‑1 (MQ=255)
                                                                         gctgcCTGCGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTa                         >  2:5174405/1‑101 (MQ=255)
                                                                          ctgcctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTAc                        <  1:5174159/101‑1 (MQ=255)
                                                                          ctgcctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTAc                        <  1:5174039/101‑1 (MQ=255)
                                                                             cctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTgg                     <  1:5173678/101‑1 (MQ=255)
                                                                              ctgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGt                    >  1:5174413/1‑101 (MQ=255)
                                                                               tgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGt                          <  1:5173797/94‑1 (MQ=255)
                                                                               tgcGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGtt                   >  2:5174415/1‑101 (MQ=255)
                                                                                 cGATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGa                 <  2:5173978/101‑1 (MQ=255)
                                                                                  gATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGAt                >  1:5174421/1‑101 (MQ=255)
                                                                                  gATATCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGAt                >  2:5174420/1‑101 (MQ=255)
                                                                                   atatCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATg               <  2:5173941/101‑1 (MQ=255)
                                                                                     atCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTa             >  1:5174426/1‑101 (MQ=255)
                                                                                      tCAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTAc            >  2:5174431/1‑101 (MQ=255)
                                                                                       cAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTAct           <  2:5174071/101‑1 (MQ=255)
                                                                                       cAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTAct           <  2:5174131/101‑1 (MQ=255)
                                                                                       cAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTAct           >  1:5174433/1‑101 (MQ=255)
                                                                                       cAATCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTAct           <  2:5173720/101‑1 (MQ=255)
                                                                                          tCCCCTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTActgct        >  1:5174438/1‑101 (MQ=255)
                                                                                           ccccTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTActgctg       <  1:5173664/101‑1 (MQ=255)
                                                                                            cccTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTActgc         >  1:5174441/1‑98 (MQ=255)
                                                                                             ccTTGTTGCCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGAc     >  2:5174442/1‑101 (MQ=255)
                                                                                               ttgttgcCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAg   <  2:5173736/101‑1 (MQ=255)
                                                                                               ttgttgcCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAg   >  2:5174447/1‑101 (MQ=255)
                                                                                                tgttgcCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGt  <  2:5173916/101‑1 (MQ=255)
                                                                                                tgttgcCCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGt  <  2:5173853/101‑1 (MQ=255)
                                                                                                     |                                                                                               
TACCGTAGCCGCGCAGCGCGTTACCAGCATAAACAACGCGGCCATCTGCGGTCGCGATAATTGCCTGTCCTTTGCTGCCTGCGATATCAATCCCCTTGTTG‑CCCCCCTCAGAAGCGCCAAAGGTTTCGATCACTTTGCCCTCAGTCGGCCAGCGCCAGGTGGAGATAGGCGTACTGGTTGATGTACTGCTGACAGT  >  NC_000913/2867820‑2868015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A