Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F13 I1 R1 116 362.0 17214306 99.7% 17162663 98.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,984,393 T→C intergenic (‑269/+18) kduI ← / ← yqeF 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase/putative acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,984,3930TC87.6% 921.3 / 102.5 300intergenic (‑269/+18)kduI/yqeF5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase/putative acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (17/20);  new base C (137/124);  total (154/146)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 301 total aligned reads displayed.

AATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAC  >  NC_000913/2984293‑2984490
                                                                                                    |                                                                                                 
aaTATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGt                                                                                                   >  1:5394963/1‑101 (MQ=255)
  tatGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCaa                                                                                                 <  2:5394529/101‑1 (MQ=255)
   atGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAag                                                                                                >  1:5394971/1‑101 (MQ=255)
   atGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAag                                                                                                >  2:5394972/1‑101 (MQ=255)
    tGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAagg                                                                                               <  2:5394319/101‑1 (MQ=255)
      tCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCTACAGATTAATGACGGCAGATATTTATCGGCAaggag                                                                                             <  2:5394570/101‑1 (MQ=255)
      tCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAaggag                                                                                             <  2:5394288/101‑1 (MQ=255)
       cATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAaggagg                                                                                            >  1:5394977/1‑101 (MQ=255)
        aTGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAggagga                                                                                           <  1:5394343/101‑1 (MQ=255)
         tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGaa                                                                                          <  2:5394339/101‑1 (MQ=255)
         tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGaa                                                                                          <  1:5394516/101‑1 (MQ=255)
         tGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGaa                                                                                          >  1:5394980/1‑101 (MQ=255)
           agaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTGGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAAt                                                                                        <  2:5394518/101‑1 (MQ=255)
            gaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATg                                                                                       <  1:5394400/101‑1 (MQ=255)
            gaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATg                                                                                       <  1:5394407/101‑1 (MQ=255)
            gaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATg                                                                                       <  1:5394363/101‑1 (MQ=255)
            gaATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATg                                                                                       <  1:5394386/101‑1 (MQ=255)
               tGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCag                                                                                    >  2:5394983/1‑101 (MQ=255)
               tGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCag                                                                                    >  1:5394984/1‑101 (MQ=255)
                 gAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCagag                                                                                  <  2:5394499/101‑1 (MQ=255)
                 gAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCaga                                                                                   <  2:5394064/100‑1 (MQ=255)
                  aaaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGc                                                                                 <  1:5394549/101‑1 (MQ=255)
                  aaaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGc                                                                                 >  2:5394990/1‑101 (MQ=255)
                   aaaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCt                                                                                >  1:5394992/1‑101 (MQ=255)
                    aaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGc                                                                                 <  1:5394315/99‑1 (MQ=255)
                    aaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTa                                                                               <  2:5394560/101‑1 (MQ=255)
                    aaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTa                                                                               <  2:5394468/101‑1 (MQ=255)
                    aaaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTa                                                                               <  1:5394253/101‑1 (MQ=255)
                     aaTGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTAt                                                                              <  1:5394140/101‑1 (MQ=255)
                        gAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCg                                                                           <  2:5394448/101‑1 (MQ=255)
                        gAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCg                                                                           >  1:5394997/1‑101 (MQ=255)
                         aGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGt                                                                          <  2:5394164/101‑1 (MQ=255)
                            gAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCAc                                                                       >  2:5395000/1‑101 (MQ=255)
                             aGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACg                                                                      <  2:5394541/101‑1 (MQ=255)
                              gCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGt                                                                     >  2:5395003/1‑101 (MQ=255)
                               cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCaga                                                                                   <  2:5394257/86‑1 (MQ=255)
                               cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGtt                                                                    <  2:5394704/101‑1 (MQ=255)
                               cccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGtt                                                                    >  1:5395004/1‑101 (MQ=255)
                                ccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTc                                                                   <  2:5394410/101‑1 (MQ=255)
                                ccAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTc                                                                   <  2:5393718/101‑1 (MQ=255)
                                 cAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCa                                                                  >  1:5395009/1‑101 (MQ=255)
                                  aGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGtt                                                                    <  1:5394457/98‑1 (MQ=255)
                                  aGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCaa                                                                 >  1:5395012/1‑101 (MQ=255)
                                  aGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCaa                                                                 >  2:5395013/1‑101 (MQ=255)
                                   gAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAAt                                                                <  1:5394778/101‑1 (MQ=255)
                                   gAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAAt                                                                <  1:5394487/101‑1 (MQ=255)
                                     aaaTGTATGTCGAGTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAAt                                                                >  1:5395017/1‑99 (MQ=255)
                                      aaTGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGt                                                             <  1:5394638/101‑1 (MQ=255)
                                         gtatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGTCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCaa                                                          <  1:5394437/101‑1 (MQ=255)
                                         gtatgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCaa                                                          >  2:5395020/1‑101 (MQ=255)
                                            tgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGc                                                       <  1:5394563/101‑1 (MQ=255)
                                            tgtCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGc                                                       >  2:5395024/1‑101 (MQ=255)
                                              tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCa                                                           >  2:5395028/1‑95 (MQ=255)
                                              tCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCca                                                     >  1:5395027/1‑101 (MQ=255)
                                               cGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCcac                                                    <  2:5394368/101‑1 (MQ=255)
                                                gACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCcaca                                                   >  2:5395032/1‑101 (MQ=255)
                                                 aCTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCTCGTTCAATGGTCAATGCCacac                                                  >  1:5395035/1‑101 (MQ=255)
                                                 aCTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCacac                                                  <  1:5394354/101‑1 (MQ=255)
                                                   ttGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATg                                                               >  1:5395039/1‑86 (MQ=255)
                                                   ttGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACAccc                                                >  2:5395037/1‑101 (MQ=255)
                                                    tGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt                                               <  1:5394023/101‑1 (MQ=255)
                                                    tGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt                                               <  2:5394599/101‑1 (MQ=255)
                                                    tGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt                                               <  2:5394586/101‑1 (MQ=255)
                                                     gccagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTg                                              >  2:5395044/1‑101 (MQ=255)
                                                       cagccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGc                                            >  1:5395046/1‑101 (MQ=255)
                                                        agccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                           >  1:5395051/1‑101 (MQ=255)
                                                        agccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                           <  2:5394383/101‑1 (MQ=255)
                                                        agccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGcc                                           >  2:5395049/1‑101 (MQ=255)
                                                         gccaTCATTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCcaca                                                   <  1:5394475/92‑1 (MQ=255)
                                                           catcatTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGccccc                                        >  1:5395056/1‑101 (MQ=255)
                                                           catcatTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGccccc                                        <  2:5394640/101‑1 (MQ=255)
                                                            atcatTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCt                                               >  2:5395061/1‑93 (MQ=255)
                                                            atcatTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccg                                       >  1:5395060/1‑101 (MQ=255)
                                                            atcatTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccg                                       <  1:5394293/101‑1 (MQ=255)
                                                              catTATTCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCccgcc                                     >  1:5395064/1‑101 (MQ=255)
                                                                 tattCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGAt                                  >  1:5395068/1‑101 (MQ=255)
                                                                  attCAACAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATa                                 >  2:5395070/1‑101 (MQ=255)
                                                                      aaCAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGACACaaa                             >  1:5395075/1‑101 (MQ=255)
                                                                       aCAGATTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAg                            >  2:5395076/1‑101 (MQ=255)
                                                                           aTTAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGtt                        >  2:5395081/1‑101 (MQ=255)
                                                                            ttAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTg                       >  1:5395086/1‑101 (MQ=255)
                                                                            ttAATGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTg                       >  1:5395083/1‑101 (MQ=255)
                                                                              aaTGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGcc                     >  1:5395089/1‑101 (MQ=255)
                                                                               aTGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCa                    >  2:5395092/1‑101 (MQ=255)
                                                                                tGACGGCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAg                   >  1:5395094/1‑101 (MQ=255)
                                                                                    ggCAGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCt               >  2:5395098/1‑101 (MQ=255)
                                                                                       aGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTg            >  1:5395102/1‑101 (MQ=255)
                                                                                       aGATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTg            <  1:5394583/101‑1 (MQ=255)
                                                                                        gATATTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGc           >  2:5395103/1‑101 (MQ=255)
                                                                                          tatTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCgg         >  1:5395108/1‑101 (MQ=255)
                                                                                          tatTTATCGGCAAGGAGAAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCgg         <  1:5394672/101‑1 (MQ=255)
                                                                                           atTTATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATAc                                >  1:5395111/1‑77 (MQ=255)
                                                                                            tttATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACCCCCTGGCCCCCGCCGATa                                 <  2:5394916/75‑1 (MQ=255)
                                                                                            tttATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGc       >  2:5395113/1‑101 (MQ=255)
                                                                                             ttATCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCa      >  2:5395116/1‑101 (MQ=255)
                                                                                               aTCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCttt             <  1:5394647/92‑1 (MQ=255)
                                                                                               aTCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCAtt    <  1:5394714/101‑1 (MQ=255)
                                                                                                tCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTc                 <  1:5394504/87‑1 (MQ=255)
                                                                                                tCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCa                    <  1:5394619/84‑1 (MQ=255)
                                                                                                tCGGCAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTa   >  1:5395119/1‑101 (MQ=255)
                                                                                                    |                                                                                                 
AATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGCAGAGCTATTCGTCACGTTCAATGGTCAATGCCACACCCTGGCCCCCGCCGATACAAAGCGTTGCCAGTCCTTTGCGGGCATTAC  >  NC_000913/2984293‑2984490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A