Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A0 F0 I1 R1	113	306.8	9564096	98.5%	9420634	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,237	A→G	intergenic (‑69/‑460)	U712_19205 ← / → U712_19210	Uncharacterized protein ywcI/Minor extracellular protease vpr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,237	0	A	G	98.8%	1442.4 / ‑5.6	420	intergenic (‑69/‑460)	U712_19205/U712_19210	Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand): ref base A (0/2); major base G (215/198); minor base C (4/1); total (219/201)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 420 total aligned reads displayed.

TTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGCT  >  CP006881/3725097‑3725380

ttttCTGTCCGGCGCCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGaaaa                                                                                                                                         <  2:3089522/150‑1 (MQ=255)

  ttCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGa                                                                                                                                       <  1:3110689/150‑1 (MQ=255)

   tCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGAc                                                                                                                                      >  1:256358/1‑150 (MQ=255)

    cTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGGAAAGACg                                                                                                                                     >  1:3306341/1‑150 (MQ=255)

      gTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGc                                                                                                                                   <  2:1322141/150‑1 (MQ=255)

       tCCGGCGCCACCCAGTCCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGcc                                                                                                                                  <  1:371706/150‑1 (MQ=255)

         cGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGa                                                                                                                                <  1:3043137/150‑1 (MQ=255)

         cGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACACCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGa                                                                                                                                <  1:3410107/150‑1 (MQ=255)

          ggCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAt                                                                                                                               >  1:2456458/1‑150 (MQ=255)

          ggCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAt                                                                                                                               >  2:2470034/1‑150 (MQ=255)

          ggCGCCCCCCAGTTCATCAAACAGACCATCCTCACCCGTAAAGATCCAAGCAACCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCGGAt                                                                                                                               <  2:1659741/150‑1 (MQ=255)

             gTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCAGCCTTTTTAGCTGCCCGTTTTCTCAGCCGCTGCCCGCAAGGAAAGACGGCCGAtttc                                                                                                                            >  2:3966263/1‑150 (MQ=255)

                  ccAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttttc                                                                                                                       >  2:636246/1‑150 (MQ=255)

                    aGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTCCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTtctc                                                                                                                     <  2:3823076/150‑1 (MQ=255)

                     gTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg                                                                                                                    <  2:4677623/150‑1 (MQ=255)

                           caAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACt                                                                                                              >  1:1116498/1‑150 (MQ=255)

                            aaaaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGGCGGCCGATTTCTTTTCTCGCAAACTa                                                                                                             >  2:2841551/1‑150 (MQ=255)

                             aaaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGATTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAg                                                                                                            <  2:446532/150‑1 (MQ=255)

                              aaaGACCATCCACACCCGTACAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGc                                                                                                           <  1:3870074/150‑1 (MQ=255)

                               aaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCg                                                                                                          >  1:2947134/1‑150 (MQ=255)

                                   ccATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                                                                      <  1:673116/150‑1 (MQ=255)

                                        cacaCCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                                                                                 >  2:1883309/1‑150 (MQ=255)

                                         acacCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaa                                                                                                >  2:400033/1‑150 (MQ=255)

                                          cacCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaa                                                                                               >  2:2329433/1‑150 (MQ=255)

                                           ccccGTAAAGATCCAAGCAAACGTTTCAAAGAAAAACATCCCTCCCCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCACAGCCGCAGCCCGCAAGAAAAGGCGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa                                                                                              <  2:870750/149‑1 (MQ=255)

                                           acCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa                                                                                              <  2:1977821/150‑1 (MQ=255)

                                           acCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATGTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa                                                                                              >  1:4734077/1‑150 (MQ=255)

                                            cccGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAc                                                                                             <  1:832026/150‑1 (MQ=255)

                                             ccGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCTGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACt                                                                                            >  1:2400809/1‑150 (MQ=255)

                                              cGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTc                                                                                           <  1:4578654/150‑1 (MQ=255)

                                               gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa                                                                                          <  1:3846519/150‑1 (MQ=255)

                                               gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa                                                                                          >  2:1748106/1‑150 (MQ=255)

                                               gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa                                                                                          >  2:4722240/1‑150 (MQ=255)

                                                tAAAGATACAAGCAACCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCCTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                                                                         <  2:1229205/150‑1 (MQ=255)

                                                 aaaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGGCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGGGGCTCTCGCAAAACTCATg                                                                                        >  2:501706/1‑150 (MQ=255)

                                                 aaaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATg                                                                                        >  1:1861142/1‑150 (MQ=255)

                                                 aaaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATg                                                                                        <  1:2333509/150‑1 (MQ=255)

                                                  aaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATgt                                                                                       >  1:534510/1‑149 (MQ=255)

                                                    gATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGGCGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                                                                     >  1:4330430/1‑150 (MQ=255)

                                                     aTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGActc                                                                                    <  1:3988722/150‑1 (MQ=255)

                                                      tACAAGCAAACGTTCCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGActct                                                                                   <  1:2231275/150‑1 (MQ=255)

                                                         aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATg                                                                                >  2:2956158/1‑150 (MQ=255)

                                                         aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATg                                                                                >  2:4377725/1‑150 (MQ=255)

                                                         aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATg                                                                                <  1:4690659/150‑1 (MQ=255)

                                                             aaaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAg                                                                            >  1:1579558/1‑150 (MQ=255)

                                                                  tttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGt                                                                       >  2:2381947/1‑150 (MQ=255)

                                                                  tttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGt                                                                       <  2:477172/150‑1 (MQ=255)

                                                                   ttCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGGTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACCCATGACTCTATGATAGACCGTg                                                                      >  2:4143824/1‑150 (MQ=255)

                                                                      aTAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCCCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGaa                                                                   >  2:2287018/1‑150 (MQ=255)

                                                                         gAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAaga                                                                <  1:4259686/150‑1 (MQ=255)

                                                                          aaaaaCATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGGCTCTATGATAGACCGTGGAAagag                                                               >  2:725338/1‑150 (MQ=255)

                                                                            aaaCATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAagagga                                                             >  1:1255962/1‑148 (MQ=255)

                                                                            aaaCATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACCCATGACTCTATGATAGACCGTGGGAAGAGaa                                                             >  1:720237/1‑150 (MQ=255)

                                                                             aaCATCCCTCCTCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAACCTCATGACTCTATGATAGACCGTGGAAAGAGAAt                                                            <  1:2178377/150‑1 (MQ=255)

                                                                             aaCATCCCTCCGCTTGTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAAt                                                            <  1:1780597/150‑1 (MQ=255)

                                                                             aaCATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAAt                                                            >  2:4272990/1‑150 (MQ=255)

                                                                             aaCATCCCTCCGCTTCGTTTTGGCAGGCAGCCTTTTTAGCAGCCCGGTTTCTCAGCCGCAGCCCGCAAGAAAAGGCGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAAt                                                            <  2:3261776/150‑1 (MQ=255)

                                                                              aCATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAAtt                                                           <  1:2066909/150‑1 (MQ=255)

                                                                               cATCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAATCATGACTCTATGATAGACCGTGGAAAGGGAATTc                                                          >  2:2610821/1‑150 (MQ=255)

                                                                                aTCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt                                                         >  1:2991089/1‑150 (MQ=255)

                                                                                aTCCCTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt                                                         >  1:4077788/1‑150 (MQ=255)

                                                                                 cccccccGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCACAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCtt                                                        <  1:3482019/149‑1 (MQ=255)

                                                                                  cccTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCCATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGGATTCttt                                                       >  1:2745240/1‑150 (MQ=255)

                                                                                   ccTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTg                                                      >  1:198528/1‑150 (MQ=255)

                                                                                    cTCCGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGt                                                     <  1:4017155/150‑1 (MQ=255)

                                                                                      ccGCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTca                                                   <  1:3278010/150‑1 (MQ=255)

                                                                                        gCTTCTTTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTcaca                                                 >  1:1709990/1‑150 (MQ=255)

                                                                                            cttTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAatat                                             >  1:4490791/1‑150 (MQ=255)

                                                                                            cttTTTGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAatat                                             <  1:2138688/150‑1 (MQ=255)

                                                                                              ttttGGCAGGCAGCCTTTTTAGCAGCCCGGTTTCCCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGa                                           <  1:591850/150‑1 (MQ=255)

                                                                                               tttGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGGGAATTCTTTGTCACAATATGAg                                          >  1:2615321/1‑150 (MQ=255)

                                                                                                 tGGCAGGCAGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTg                                        >  1:4372290/1‑150 (MQ=255)

                                                                                                  ggcaggcaGCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGa                                       <  1:3579797/150‑1 (MQ=255)

                                                                                                     aggcagCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaaaa                                    <  1:2253663/150‑1 (MQ=255)

                                                                                                     aggcagCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaaaa                                    <  1:937008/150‑1 (MQ=255)

                                                                                                       gcatcctttttAGCAGCCCCGTTTCCCAGCCGCAGCCCGCAAGAAAACACGGCCGATTTCTTTTCTCGCCAACTAGCGGCTCTAGCAAAACTCATGACTCTGTGATAGACCGTGGAAAGAGAATTCTTTGGCACAATATGAGTGaaaaaa                                  <  1:3618340/143‑1 (MQ=255)

                                                                                                        cagCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAc                                 <  2:1255962/150‑1 (MQ=255)

                                                                                                         agcctttttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCACACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCAAAATATGAGTGAAAAAAcc                                >  1:4150700/6‑150 (MQ=255)

                                                                                                              ttttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTa                           >  2:3698190/1‑150 (MQ=255)

                                                                                                               tttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAg                          >  1:4424590/1‑150 (MQ=255)

                                                                                                               tttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAg                          <  2:3437070/150‑1 (MQ=255)

                                                                                                               tttAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCCATGATAGACCGTGGAAAGAGAAATCTTTGTCACAATATGAGGAAAAAAACCAACTAg                          >  1:1322498/1‑150 (MQ=255)

                                                                                                                 tAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGtt                        <  1:1396688/150‑1 (MQ=255)

                                                                                                                   gcagcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGGCTCTATGATAGACCGTGGAAAGAGGATTCTTTGTCACAATATGAGTGGAAAAACCAACTAGtttt                      >  1:1489021/1‑150 (MQ=255)

                                                                                                                    cagcCCGTTTCCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCACTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGttttt                     <  2:3009194/150‑1 (MQ=255)

                                                                                                                      gcCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGTGTGAAAAGACCAACTAGATTTTAg                   >  1:3916296/1‑150 (MQ=255)

                                                                                                                       cccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCATTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGa                  <  1:1168553/150‑1 (MQ=255)

                                                                                                                        ccGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAACAAACCAACTAGTTTTTAGaa                 >  1:2017737/1‑150 (MQ=255)

                                                                                                                        ccGTTTTCTCAGCCGCAGCCCGAAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGaa                 <  1:3172378/150‑1 (MQ=255)

                                                                                                                          gTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAAGTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGt               >  2:1515457/1‑150 (MQ=255)

                                                                                                                          gTTTCCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAACCTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGt               <  2:1177182/150‑1 (MQ=255)

                                                                                                                           ttttCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGtt              <  2:3152226/150‑1 (MQ=255)

                                                                                                                            tttCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGttt             <  1:1918302/150‑1 (MQ=255)

                                                                                                                                tcAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTttgtt         >  1:4637597/1‑150 (MQ=255)

                                                                                                                                  aGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGa       >  1:2703817/1‑150 (MQ=255)

                                                                                                                                   gCCGCAGCCCGCAAGAAAAGCCGGCCGGTTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACACATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGaa      <  2:2190329/150‑1 (MQ=255)

                                                                                                                                    ccGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGaat     >  1:1000517/1‑149 (MQ=255)

                                                                                                                                    ccGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGaaa     <  2:2550056/150‑1 (MQ=255)

                                                                                                                                      gCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGc   <  2:2711633/150‑1 (MQ=255)

                                                                                                                                       cAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGCt  <  2:1103463/150‑1 (MQ=255)

TTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCAGCC‑TTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAACCAACTAGTTTTTAGAAGTTTTGTTGAAAGCT  >  CP006881/3725097‑3725380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A