Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F63 I0 R2	103	91.4	3852028	97.4%	3751875	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,606,207:1	+G	100%	noncoding (80/87 nt)	leuP ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,207	1	.	G	88.8%	287.0 / 34.5	89	noncoding (80/87 nt)	leuP	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (5/5); new base G (48/31); total (53/36)
Fisher's exact test for biased strand distribution p-value = 5.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAA  >  NC_000913/4606068‑4606337

gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTgag‑a                                                                                                                                        >  1:646046/1‑140 (MQ=255)

gTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCATCGATTGGTGCG‑a                                                                                                                                        >  1:451641/1‑140 (MQ=255)

      aTAATTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGAAAGGACACTAACACCTGCAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTGGATATCGCCTCGTTTGGTGCG‑A‑‑g‑gg‑gggg                                                                                                                             <  2:358/140‑1 (MQ=21)

      aTAATTGGGGCG‑CGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑‑g‑gg‑gggg                                                                                                                             <  2:211/140‑1 (MQ=255)

        aaTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCACGTTTGGTGCG‑A‑‑g‑gg‑ggggg                                                                                                                            <  2:1316227/140‑1 (MQ=255)

        aaTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAATCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑‑g‑gg‑gggggg                                                                                                                           >  2:942785/1‑139 (MQ=255)

          ttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAGCTTACTTTTTTTGATATCGAATCGTTTGGTGCG‑A‑‑g‑gg‑gggggga                                                                                                                          >  2:113795/1‑138 (MQ=255)

              tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTg                                                                                                                       >  1:463163/1‑140 (MQ=255)

               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGa                                                                                                                      <  1:1255699/140‑1 (MQ=255)

               gCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCATCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGa                                                                                                                      <  1:549739/140‑1 (MQ=255)

                  aGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAAcc                                                                                                                   >  1:1306728/1‑140 (MQ=255)

                          gACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTc                                                                                                           <  1:1155440/140‑1 (MQ=255)

                          gACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTc                                                                                                           <  1:226352/140‑1 (MQ=255)

                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                     <  1:709233/140‑1 (MQ=255)

                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                     <  1:709208/140‑1 (MQ=255)

                                aaCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                     <  1:709456/140‑1 (MQ=255)

                                 aCCCCCACGTCCGTAAGGACACTAACACCTGATGCTAGCGCGTCTACCATTTCCGCCACCTTCTCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCG‑A‑‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGaa                                                                                                   <  1:84631/140‑1 (MQ=255)

                                 aCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTATACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGaa                                                                                                   <  1:85030/140‑1 (MQ=255)

                                     ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAacac                                                                                                >  1:1382862/1‑140 (MQ=255)

                                     ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAacac                                                                                                >  1:1383275/1‑140 (MQ=255)

                                     ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTAGATAACGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGACCGTAAGAacac                                                                                                >  1:3080/1‑140 (MQ=25)

                                     ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGAAATATTACTTTTTTTGATATCGAATCGATTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACACCCACGACAGTAATAacac                                                                                                >  1:1382767/1‑140 (MQ=18)

                                      cACGTCCGTAAGCACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACATCTTACTTTTTTTGATATCGCCACCTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                               <  1:455112/140‑1 (MQ=37)

                                       aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTa                                                                                              >  2:13698/1‑140 (MQ=255)

                                       aCGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTAATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGTCTTCAACCCCCACGTCCTTCAGAACACTa                                                                                              >  2:13670/1‑140 (MQ=25)

                                        cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                             <  1:1495769/140‑1 (MQ=255)

                                        cGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                             <  1:430275/140‑1 (MQ=255)

                                          tCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCGCGTGTGGTGCG‑A‑‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAacac                                                                                          >  1:341006/1‑140 (MQ=255)

                                            cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑‑G‑GGAGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                         <  2:188981/140‑1 (MQ=255)

                                            cGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCATCGTATGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTCACAcc                                                                                         >  2:1515897/1‑140 (MQ=37)

                                              tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGATCCCCCACGTCCGTAAGAACACTAACACCTg                                                                                       >  2:1356705/1‑140 (MQ=255)

                                              tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                         >  1:915520/1‑138 (MQ=255)

                                              tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                         <  2:915520/138‑1 (MQ=255)

                                              tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGACCGTAAGAACACTAAAACCTg                                                                                       >  2:493183/1‑140 (MQ=255)

                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGG‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACAAACACCTGAAGCTAgc                                                                               >  2:1464506/1‑140 (MQ=255)

                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgcg                                                                              >  1:1782488/1‑140 (MQ=255)

                                                       tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACATCTTACTTTTTTTGATATCGAAGCGTTTGGTGGG‑A‑‑GTGG‑GGGGGACTTGAACACCCACTTCCGAAAGAAGAATAACACCTGATGCTAgag                                                                              >  2:1749171/1‑138 (MQ=25)

                                                       tAACACCTGAAGCTAGCGCGTCTACCAAGTCCGCCTCCTTCGCACAGGCATCTAGGTTTTTTTTCTTTCGGGGCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGTACCCCCACGTCCGTATGTACAGTGAGACCTGAAGGTAgcg                                                                              >  2:1209292/1‑140 (MQ=16)

                                                        aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCTTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGTACACTAACACCTGAAGCTAgcgc                                                                             <  1:1680885/140‑1 (MQ=255)

                                                           acCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTc                                                                          <  2:1689925/140‑1 (MQ=255)

                                                            ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCt                                                                         >  2:1814289/1‑140 (MQ=255)

                                                            ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTGTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCAGTCTGTACACTAACACCAGAAGCTAGCGCGTCt                                                                         >  2:1923777/1‑140 (MQ=37)

                                                             cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTa                                                                        >  1:1899683/1‑140 (MQ=255)

                                                             cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGAATCGTTTGGTGCG‑AG‑G‑GG‑GGGGGCCTTGAACACCCACGGGCGTAATAAAACTAAAACCAGAAGCTATACCGTCTa                                                                        >  1:1412735/1‑140 (MQ=25)

                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGG‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAc                                                                       >  1:389631/1‑140 (MQ=255)

                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                      <  2:938619/140‑1 (MQ=255)

                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                      >  2:1666768/1‑140 (MQ=255)

                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTAca                                                                      >  1:704058/1‑139 (MQ=255)

                                                               gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGACTTCTTACTTTTTTTGATATCGCATCGGTTGGTGGG‑A‑‑GTGG‑GGGGGAATTGAACAACAACTTCAGTATGATCACTCTCTCCTGAAGCTAGCGCGTCTAcc                                                                      >  2:1757071/1‑140 (MQ=19)

                                                                    tAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                                                                 >  1:1508121/1‑140 (MQ=255)

                                                                     aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                >  2:1442913/1‑140 (MQ=255)

                                                                     aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                >  1:1374191/1‑140 (MQ=255)

                                                                       ctagTCTACCTTTTCCGGCTCCTTCGCACATTGATCTTAATTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCAACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                              <  2:834425/137‑1 (MQ=18)

                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCAACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                              <  2:837403/140‑1 (MQ=255)

                                                                       cgcgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCAACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                              <  2:1477577/140‑1 (MQ=255)

                                                                           tcgaCCAATTCCGCCACCTTCGCACAGGCATCTTACTATTTTTGTTATCGCCTCGATAGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                          >  2:1837784/4‑140 (MQ=21)

                                                                           tCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                          <  1:144360/140‑1 (MQ=255)

                                                                           tCTACCAATTCCGCCACCTTCGCACAGTCAGCTTACATTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACTTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATACCGCCAcc                                                          >  2:1838609/1‑140 (MQ=21)

                                                                                 aaTTCCGCCACTTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                    <  1:1546441/140‑1 (MQ=255)

                                                                                 aaTTCCGCCACTTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                    <  1:1375840/140‑1 (MQ=255)

                                                                                 aaTTCCGCCACCTTCGCACAGTCAACTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                    <  1:1338469/140‑1 (MQ=255)

                                                                                     ccGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt                                                <  2:1374191/140‑1 (MQ=255)

                                                                                      cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTg                                               <  1:516108/140‑1 (MQ=255)

                                                                                        ccACCTTCGCACATTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                             <  2:801797/140‑1 (MQ=255)

                                                                                        ccACCTTCGCACAGGCATCTTACTTTTTTTGATATCGCCTCGGTTGGTGCG‑AGGG‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGc                                              >  1:559870/1‑140 (MQ=21)

                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                           <  1:536616/140‑1 (MQ=255)

                                                                                          aCCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                           <  1:824962/140‑1 (MQ=255)

                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                               <  2:664697/76‑1 (MQ=255)

                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                               >  1:664697/1‑76 (MQ=255)

                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                               >  2:1601066/1‑140 (MQ=255)

                                                                                                      tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAAACCCCACGTCCGTAAGAAAACTAACACCTGAAGCTATCGCGGCAACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAATTCAACgg                               >  1:1481887/1‑139 (MQ=21)

                                                                                                          cTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtt                           >  1:445397/1‑140 (MQ=255)

                                                                                                           ttACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt                         <  2:1624084/140‑1 (MQ=255)

                                                                                                              cTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTTTTg                       >  2:356367/1‑140 (MQ=255)

                                                                                                              cTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                       >  2:1558093/1‑140 (MQ=255)

                                                                                                              cTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                       >  1:446267/1‑140 (MQ=255)

                                                                                                              cTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                       >  1:446095/1‑140 (MQ=255)

                                                                                                              cTTTTTTTGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg                       >  2:492519/1‑140 (MQ=255)

                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTCTATGGt                     >  2:1425579/1‑140 (MQ=21)

                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                     >  2:1876129/1‑140 (MQ=255)

                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                     >  2:1876326/1‑140 (MQ=255)

                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGt                     >  2:1503829/1‑140 (MQ=255)

                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGAGAGCAATATCAAAGTGGTTTTTGGt                     >  2:236114/1‑140 (MQ=255)

                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAATATCAACGTGGTTTTTGGt                     >  2:1590828/1‑140 (MQ=255)

                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAATCCGCCACCTTCGCCCAGTGCGAGCAATATAAACGTGGTTTTTGGt                     >  2:1085036/1‑140 (MQ=255)

                                                                                                                ttttttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAAAACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGTGGTGTTTGGt                     >  2:1401134/1‑140 (MQ=21)

                                                                                                                tattttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGAAAGAACACTAACACCTGAAGCTAGCGCGACTACCAATTCCGCCACCTTCGCCCAGAGCGAGCAAAATCAACGAGGTTTTAGGt                     >  2:1590834/3‑140 (MQ=18)

                                                                                                                    ttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACTCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                 >  2:165628/1‑140 (MQ=255)

                                                                                                                    ttGATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                 >  2:661577/1‑140 (MQ=255)

                                                                                                                      gATATCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTACCACCTGAAGCTCGCGCGTATACCAATTCCGACACATTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgg               <  1:1099222/140‑1 (MQ=21)

                                                                                                                         atCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg            <  1:436623/140‑1 (MQ=255)

                                                                                                                         atCGCCTCGTTTGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGG‑TTTTGGTGCGAgggggg           <  2:526107/140‑1 (MQ=255)

                                                                                                                          tCGCCTCGTTTGGTGCGTG‑‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTACCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg           <  1:609491/140‑1 (MQ=255)

                                                                                                                               tCGTTTGGTGCG‑A‑‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACtt     >  1:104231/1‑140 (MQ=255)

                                                                                                                                  tttGGTGCG‑AG‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGa   <  1:661577/140‑1 (MQ=255)

                                                                                                                                  tgtGGTGCG‑A‑‑G‑GG‑GGGGGACTTCAACCCCCACGGCCGGAAGAACACTAACACCAGAAGCTAGCGCGTCTACCAATTCCGCCCCCAGCCCCCACAGCCACCAATATCAACGGGGGTGTTGGTTCGAGGGGCGGGACTAGaa  >  2:601974/3‑140 (MQ=9)

GTAAAGATAATTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑A‑‑G‑GG‑GGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAA  >  NC_000913/4606068‑4606337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A