Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F60 I0 R2 105 83.0 3386788 97.3% 3295344 139.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,233,642 G→A 13.6% intergenic (‑409/‑116) lysC ← / → pgi lysine‑sensitive aspartokinase 3/glucosephosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,233,6420GA13.6% 248.2 / 27.8 89intergenic (‑409/‑116)lysC/pgilysine‑sensitive aspartokinase 3/glucosephosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (48/28);  new base A (6/6);  total (55/34)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.98e-01

CGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACG  >  NC_000913/4233504‑4233778
                                                                                                                                          |                                                                                                                                        
cgcgCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgc                                                                                                                                         >  2:1224719/1‑140 (MQ=255)
cgcgCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgc                                                                                                                                         >  2:660254/1‑140 (MQ=255)
cgcgCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgc                                                                                                                                         >  1:1348660/1‑140 (MQ=255)
cgcgCCTCTTATGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgc                                                                                                                                         >  2:1384126/1‑140 (MQ=255)
 gcgcCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTATTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTgcg                                                                                                                                        >  1:1536708/1‑140 (MQ=255)
    ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  1:918020/140‑1 (MQ=255)
    ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  2:421345/140‑1 (MQ=255)
    ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  2:316906/140‑1 (MQ=255)
    ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  1:1580653/140‑1 (MQ=255)
    ccTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  2:1338733/140‑1 (MQ=255)
      tcttctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAg                                                                                                                                   <  2:1593767/140‑1 (MQ=255)
       cttctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAgg                                                                                                                                  >  2:1529412/1‑140 (MQ=255)
         tctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGt                                                                                                                                <  1:1144006/140‑1 (MQ=255)
         tctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGt                                                                                                                                <  2:1035047/140‑1 (MQ=255)
         tctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGt                                                                                                                                <  1:1143727/140‑1 (MQ=255)
          ctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGtt                                                                                                                               >  1:1276816/1‑140 (MQ=255)
          ctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGtt                                                                                                                               >  1:1276428/1‑140 (MQ=255)
          ctGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGtt                                                                                                                               >  1:1276397/1‑140 (MQ=255)
            ggCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTa                                                                                                                             <  1:111376/140‑1 (MQ=255)
             gCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTac                                                                                                                            >  1:31509/1‑140 (MQ=255)
             gCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTGTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTac                                                                                                                            >  1:1272131/1‑140 (MQ=255)
              ccTGCGCTAGCGCAGGTAGTACAATTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTTATAAATGCTTCACTGCGCTAAGGGTTTaca                                                                                                                           <  1:585594/140‑1 (MQ=255)
                tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCTTGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACt                                                                                                                         <  1:659122/140‑1 (MQ=255)
                tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACt                                                                                                                         <  2:1585433/140‑1 (MQ=255)
                tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACt                                                                                                                         <  1:1017273/140‑1 (MQ=255)
                tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACt                                                                                                                         <  1:1612474/140‑1 (MQ=255)
                tGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACt                                                                                                                         <  1:832386/140‑1 (MQ=255)
                  cgcTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATTCTTCACTGCGCTAAGGGTTTACACTCa                                                                                                                       >  2:728064/1‑140 (MQ=255)
                  cgcTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCa                                                                                                                       >  2:345730/1‑140 (MQ=255)
                  cgcTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCa                                                                                                                       >  2:751576/1‑140 (MQ=255)
                      aGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACAt                                                                                                                   <  2:1330291/140‑1 (MQ=255)
                            ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTTGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  1:82381/1‑140 (MQ=255)
                            ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  1:868409/1‑140 (MQ=255)
                            ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  2:802417/1‑140 (MQ=255)
                            ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  1:348885/1‑140 (MQ=255)
                            ggTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCt                                                                                                             >  2:1280465/1‑140 (MQ=255)
                              tagtaCATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTaa                                                                                                           <  1:1683222/140‑1 (MQ=255)
                                  aCATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGAAGTTCAGCTAATAAATGCTTCACTGAGCTAAGGGTTTACACTCAACAATACGCTAACGGc                                                                                                       >  2:1678468/1‑140 (MQ=255)
                                   cATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     >  1:772981/1‑109 (MQ=255)
                                   cATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTa                                                                                                                                     <  2:772981/109‑1 (MQ=255)
                                       tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:579433/1‑140 (MQ=255)
                                       tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:1189774/1‑140 (MQ=255)
                                       tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:1189739/1‑140 (MQ=255)
                                       tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:1418258/1‑140 (MQ=255)
                                       tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  2:1078427/1‑140 (MQ=255)
                                       tataAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTaa                                                                                                  >  1:225081/1‑140 (MQ=255)
                                           aataaaGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAAcc                                                                                              >  2:1140197/1‑140 (MQ=255)
                                              aaaGGGTGAGCGGGGCGGTTGTCAACTATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAATCCATc                                                                                           <  2:877851/140‑1 (MQ=255)
                                              aaaGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCTGATTTTTCATCCACTCCTTGCGGTCAGTAGTTCAGCTAATAAATGCTTCTCTACGCTAAGTGTTTACACTCAACTTTACGCTTACGGCACTACAACCATc                                                                                           <  2:244900/140‑1 (MQ=255)
                                                cgggTGAGCCGGGCGGATGTCAACGATTGGGTCATGCGGAATTTTCATCCACTACTGGCGGTCACTATTTCAGCTAATAAATGCTTCACAGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAAACATcac                                                                                         >  2:566307/2‑140 (MQ=255)
                                                aGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATcac                                                                                         >  1:1131175/1‑140 (MQ=255)
                                                aGGGTGAGCGGGGAGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGGTTACACTCAACAGTACGCTAACGGCACTAAAAACATcac                                                                                         >  2:566312/1‑140 (MQ=255)
                                                  ggTGAGCGGGGCGGTTGTCAACGTTGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTATCGGCACTAAATCCATCACAt                                                                                       <  1:697437/140‑1 (MQ=255)
                                                      aGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAttttt                                                                                   >  1:787493/1‑140 (MQ=255)
                                                      aGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAttttt                                                                                   >  1:1662549/1‑140 (MQ=255)
                                                      aGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAttttt                                                                                   >  1:1633576/1‑140 (MQ=255)
                                                            gCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGa                                                                             >  1:1573812/1‑140 (MQ=255)
                                                             cGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGAc                                                                            <  1:451342/140‑1 (MQ=255)
                                                                     aaCGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGTCGCTa                                                                    >  1:786611/1‑140 (MQ=255)
                                                                       cGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACa                                                                  <  2:1470577/140‑1 (MQ=255)
                                                                       cGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACa                                                                  <  2:1470477/140‑1 (MQ=255)
                                                                          tGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGTGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACAATTTTCTGTGACTGGCGCTACAATc                                                               >  2:1531818/1‑140 (MQ=255)
                                                                                  tGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAg                                                       >  1:762645/1‑140 (MQ=255)
                                                                                  tGCGGATTTTTCATCCACTCCTGGCGGTAAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACAATACGCTAACGGCACTAAAACCATCACATTTATCTGTGACTGGAGCTACAATCTTCCAAAg                                                       >  2:413823/1‑140 (MQ=255)
                                                                                         ttttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAAt                                                >  1:749703/1‑140 (MQ=255)
                                                                                         ttttCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAAt                                                >  1:519675/1‑140 (MQ=255)
                                                                                             cATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACA‑TTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCa                                           >  2:762032/1‑140 (MQ=255)
                                                                                                  aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAAt                                       <  1:1118951/140‑1 (MQ=255)
                                                                                                  aCTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAAt                                       <  1:1604550/140‑1 (MQ=255)
                                                                                                   cTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATc                                      >  1:1100956/1‑140 (MQ=255)
                                                                                                     ccTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCag                                    <  1:370319/140‑1 (MQ=255)
                                                                                                              cAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCATAGTCACAATTCTCAAAATCAGAAGAGTAtt                           >  1:1362760/1‑140 (MQ=255)
                                                                                                              cAGTAGTTCAGCTAATAAATGCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTAtt                           >  2:809961/1‑140 (MQ=255)
                                                                                                              cAGTAGTTCAGCAAATAAATCCTTCACTTCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACAAAAACCATCACAATTTTCAGTCACTGGCGCTACAAACAACCAAAGACAAAAATCTCAAAATCAGAAtattatg                           >  2:598238/1‑139 (MQ=255)
                                                                                                                           aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAAc              >  2:669849/1‑140 (MQ=255)
                                                                                                                           aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAAc              >  2:1098973/1‑140 (MQ=255)
                                                                                                                           aataaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAAc              >  2:1098977/1‑140 (MQ=255)
                                                                                                                             taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAt            <  2:1536708/140‑1 (MQ=255)
                                                                                                                             taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAt            <  1:1232727/140‑1 (MQ=255)
                                                                                                                             taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAt            <  2:1647059/140‑1 (MQ=255)
                                                                                                                                  gCTTCACTACGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATc       <  2:1027238/140‑1 (MQ=255)
                                                                                                                                   cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATcc      >  2:346173/1‑140 (MQ=255)
                                                                                                                                   cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATcc      >  1:411209/1‑140 (MQ=255)
                                                                                                                                   cTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATcc      >  2:346484/1‑140 (MQ=255)
                                                                                                                                    ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCa     <  2:1661615/140‑1 (MQ=255)
                                                                                                                                      cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:199520/1‑140 (MQ=255)
                                                                                                                                      cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:140328/1‑140 (MQ=255)
                                                                                                                                      cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:1639646/1‑140 (MQ=255)
                                                                                                                                      cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:1639656/1‑140 (MQ=255)
                                                                                                                                      cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:479469/1‑140 (MQ=255)
                                                                                                                                      cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:1126070/1‑140 (MQ=255)
                                                                                                                                      cACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAAc   >  1:199506/1‑140 (MQ=255)
                                                                                                                                      cACTGCGCTAAGGGTTTACACTCAACATAACGCTAACGGCACTAAAACCATCACATTATTCTGTGACTGGCGATACAATCTTCCAAAGTCACAATTCTCAAAAACAGAAGAGTATTGCTAATGAAAAACAACAATCCAAc   >  1:1656942/1‑140 (MQ=255)
                                                                                                                                       aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCAACACAATATACTGTGACTGGCGCTACAAACTACCAAACTCACAATTCTCAAAATCAGAACAGTATTGCTAATGAAAAACAACAATCCAACg  <  2:448547/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                        
CGCGCCTCTTCTGGCCTGCGCTAGCGCAGGTAGTACATTTATAAATAAAGGGTGAGCGGGGCGGTTGTCAACGATGGGGTCATGCGGATTTTTCATCCACTCCTGGCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACG  >  NC_000913/4233504‑4233778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A