Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F53 I6 R4	121	75.7	2723416	94.9%	2584521	126.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,725,043	C→A	A42A (GCG→GCT)	U712_19205 ←	Uncharacterized protein ywcI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,725,043	0	C	A	100.0%	94.2 / NA	28	A42A (GCG→GCT)	U712_19205	Uncharacterized protein ywcI
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (5/23); total (5/23)

TCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACAT  >  CP006881/3724907‑3725178

gctcCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagc                                                                                                                                       <  1:296024/138‑1 (MQ=255)

    cTGCTTTGGCGATTGCTTGAAGGCTTGCAAACATCTACTTTTAAAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACCGCCTTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                                   <  2:222806/139‑2 (MQ=255)

    cTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc                                                                                                                                   <  5:141059/139‑2 (MQ=255)

       cTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTAGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACCAAACGAGTCCTGCGGCCAGCCCagccgccgtt                                                                                                                                >  2:7704/1‑135 (MQ=255)

          tGGCGATGCATTGAAGGCTTGCAAACATGTTCTTTTATTAGGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCTTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                              <  4:62304/138‑1 (MQ=255)

            gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                            <  3:154460/138‑1 (MQ=255)

            gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTg                                                                                                                           >  6:130631/1‑139 (MQ=255)

            gCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGAGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt                                                                                                                            <  2:111641/138‑1 (MQ=255)

                ttCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                                                       <  6:305662/139‑1 (MQ=255)

                ttCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTAt                                                                                                                       <  5:294784/139‑1 (MQ=255)

                 tCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                              <  6:141117/131‑1 (MQ=255)

                 tCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCt                                                                                                                              >  5:141117/1‑131 (MQ=255)

                       aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                <  8:89297/139‑1 (MQ=255)

                       aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt                                                                                                                <  7:259720/139‑1 (MQ=255)

                       aaGGCTTGCAAACATCTTCTTTAATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGAACGCt                                                                                                                <  2:311084/139‑1 (MQ=255)

                          gCTTGCAAACATCTTCTTTTATAACGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                             <  5:220861/139‑1 (MQ=255)

                          gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                             <  1:7704/139‑1 (MQ=255)

                          gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                             <  7:95275/139‑1 (MQ=255)

                          gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                             <  2:226706/139‑1 (MQ=255)

                          gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                             <  5:130631/139‑1 (MQ=255)

                          gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg                                                                                                             <  3:334993/139‑1 (MQ=255)

                                                  aGCGCGGCGGCGTGCGCTTTCTGCTTTTCTTCCTCATTCACACCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAg                                                                                     <  5:331014/139‑1 (MQ=255)

                                                                             tcttcCTCATTCCCAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTtc                                                           <  3:48162/138‑1 (MQ=255)

                                                                                 ccTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAAAGCCGCCGTTATTTGGTATGTTCGCTGAGCCCAATATACCGCGCCAACCGCACTTTTTCTGTCCGGCCTCACCCAGTtcatca                                                       >  1:87376/1‑138 (MQ=255)

                                                                                      ttCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGa                                                 <  5:166820/139‑1 (MQ=255)

                                                                                                tCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTGTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCccac                                       >  5:117983/1‑139 (MQ=255)

                                                                                                     cattCATAAAACGAAACGAGTCCTGAGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTa                                  <  7:291276/136‑1 (MQ=255)

                                                                                                           tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa                            <  7:13660/139‑1 (MQ=255)

                                                                                                           tAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAACCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATa                            <  2:87376/139‑1 (MQ=255)

                                                                                                                           ctgcgGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCTCGGCAGCCGCAGTTTTTCTGTCCGTCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCAt            <  5:198658/137‑1 (MQ=255)

                                                                                                                                  cTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGaaaaa     <  5:158772/139‑1 (MQ=255)

                                                                                                                                   tGCACAGCCGCCTTTCTTTGGTATGTACCCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAACACCATCCCCACCCCGAAAGATACAATCAAACGTTTCAAAGaaaaa     >  5:195497/1‑138 (MQ=255)

                                                                                                                                     cacaGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCAGTCCGGCGTAACCCCGTTCATCAAAAAGACCATCCACACCCCTAAAGAAACAACCAAACCTTTCATAGaaaaaaaa  >  8:59620/1‑136 (MQ=255)

TCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACAT  >  CP006881/3724907‑3725178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A