Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F53 I19 R3 125 83.6 3029080 95.4% 2889742 127.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,724,749 T→C P26P (CCA→CCG)  U712_19200 ← SacPA operon antiterminator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,724,7490TC98.3% 205.4 / ‑4.2 59P26P (CCA→CCG) U712_19200SacPA operon antiterminator
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (23/34);  total (23/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCG  >  CP006881/3724613‑3724882
                                                                                                                                        |                                                                                                                                     
cAGCTATTTCTATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCgg                                                                                                                                     >  4:280533/1‑139 (MQ=255)
         ctatatGCTCCTCCGGCATTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTTAAAAGCGATTCCCGGTCCCATCa                                                                                                                             <  2:355949/136‑1 (MQ=255)
             atGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGAtt                                                                                                                        >  6:264222/1‑139 (MQ=255)
                ctcctcCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAATATCTATTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTTAAAAGCGATTCCCCGTCCAATCACGAttttt                                                                                                                     >  3:314691/1‑139 (MQ=255)
                ctcctcCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATc                                                                                                                              >  6:115453/1‑130 (MQ=255)
                ctcctcCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATc                                                                                                                              <  5:115453/130‑1 (MQ=255)
                ctcctcCGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGAttttt                                                                                                                     >  2:207977/1‑139 (MQ=255)
                 tcctccGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGAttttt                                                                                                                     >  4:11250/1‑138 (MQ=255)
                   ctccGGCAGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCAAGATTTTTTcc                                                                                                                  >  5:9616/1‑139 (MQ=255)
                   atccGGCAGTGTATGCAGGATTTGATTGAACTTTTCATTATCGTCGCGCACGACAAAGATCTTTTCCAATTTATTAATATGTATGAGATCATTTTTCTTTTTTTTAAAAGCGATTACCTGTCCCATCACGAttttttac                                                                                                                  >  3:233686/2‑137 (MQ=255)
                          aGTGTTTTCAGGATTTTTTTGAACTTTTCATTCTCGTGGCGCACGACAAAGATATTTTCCA‑ATTATTCAATGGTATGAGATCATTTTTATTTTTTTTAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcatc                                                                                                          <  2:369844/139‑1 (MQ=255)
                          aGTGTTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcat                                                                                                           <  4:248871/139‑1 (MQ=255)
                           gtgtTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcatc                                                                                                          <  3:315060/139‑1 (MQ=255)
                           gtgtTTGCAGGATTTGTTTGAACTATTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcatc                                                                                                          <  1:55894/139‑1 (MQ=255)
                           gtgtTTGCAGGATTAGTTTGAACGTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcatc                                                                                                          <  2:212701/139‑1 (MQ=255)
                             gtTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCa                                                                                                                             <  3:344079/118‑1 (MQ=255)
                             gtTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCa                                                                                                                             >  4:344079/1‑118 (MQ=255)
                             gtTTGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCct                                                                                                        >  3:89885/1‑139 (MQ=255)
                                tGCAGGATTTGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCtt                                                                                                     >  5:97145/1‑139 (MQ=255)
                                 gCAGGATTTGTTTGATCTTTTCATTCTCGTCGCGCATGAATAAGATCTTTTCCTGTTTATTCATAGGGATGATATCATTTTTATTTTTTTGAAAAGCGATTCCCGGTCCCATCAGGATTTTTTCCTGATCATCCTCtt                                                                                                     <  4:314691/138‑1 (MQ=255)
                                       tttgtttgAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTttatta                                                                                               <  3:237234/138‑1 (MQ=255)
                                        ttGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTaa                                                                                              <  3:290160/138‑1 (MQ=255)
                                        ttGTTTGAACTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTATTTTTGAAAAGCTAATCCCTGTCCCAAAACGATTTATTCCTGCTCATCCTCTTttatta                                                                                               >  4:359006/1‑137 (MQ=255)
                                             tgaaCTTTTCATTCTCGGCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCg                                                                                         <  6:280347/136‑1 (MQ=255)
                                               aaCTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTAAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCa                                                                                       <  1:255890/138‑1 (MQ=255)
                                               aaCTTTTCATCCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAt                                                                                      >  8:322177/1‑139 (MQ=255)
                                                 cTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTTATTGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                                                    <  6:176490/139‑1 (MQ=255)
                                                 cTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                                                    <  5:120912/139‑1 (MQ=255)
                                                 cTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                                                    <  4:89885/139‑1 (MQ=255)
                                                 cTTTTCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                                                    <  7:337761/139‑1 (MQ=255)
                                                 cTTTTCATTCACGGCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGACATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCAttg                                                                                    <  6:170370/139‑1 (MQ=255)
                                                    ttCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcat                                                                                                           >  1:267129/1‑113 (MQ=255)
                                                    ttCATTCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGatcat                                                                                                           <  2:267129/113‑1 (MQ=255)
                                                        ttCTCGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTa                                                                             <  4:338403/139‑1 (MQ=255)
                                                          ctcGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTaa                                                                            <  4:134474/138‑2 (MQ=255)
                                                          ctcGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTaa                                                                            <  3:98106/138‑2 (MQ=255)
                                                          ctcGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTaa                                                                            <  6:232592/138‑2 (MQ=255)
                                                          ctcGTCGCGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTaa                                                                            <  1:372818/138‑2 (MQ=255)
                                                                    aagACAAAGATAGTTTCCACTTTAATACTATGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTACCGGTACCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTTTTGTTTAATACTTtatata                                                                 <  7:336913/137‑3 (MQ=255)
                                                                    aCGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGa                                                                 <  3:127329/139‑1 (MQ=255)
                                                                           aGATCTTTTCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                                                          <  7:89656/139‑1 (MQ=255)
                                                                            gATCTTTGCCACTTTATTGATAGGGATTCGATCTTTTTTCGTTTTTTTAAAAGCGATTCAGGGTCGCATCACGATTTTTTCCTGATCATACTCTTTTCTTAAAGCCGAATTGTTGTTTAATAATTTATAGATTTTCAag                                                         <  5:273147/139‑1 (MQ=255)
                                                                                ttttCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGCTCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAggt                                                     >  5:67908/1‑138 (MQ=255)
                                                                                  ttCCACTTTATTCATAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTa                                                   >  8:88806/1‑139 (MQ=255)
                                                                                            ttCATAGGGATGAGATGATTTTTCATTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTATCCTGATCATCCTCTTTTATTAAATCCGCAATGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCg                                          <  8:34209/138‑1 (MQ=255)
                                                                                                tAGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAAt                                     <  8:116875/139‑1 (MQ=255)
                                                                                                 aGGGATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCTTAAAGATTATTTCATGATCATCCTCTATTATTAAAGCCGCATTCTTGTTTAATAATTTATAGATTTTCAAGAGGATATCTCTCACGCCAAtt                                    >  8:185675/1‑139 (MQ=255)
                                                                                                    gATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                                                          >  6:35497/1‑114 (MQ=255)
                                                                                                    gATGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCaa                                                          <  5:35497/114‑1 (MQ=255)
                                                                                                      tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                               >  6:295167/1‑139 (MQ=255)
                                                                                                      tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                               >  7:280223/1‑139 (MQ=255)
                                                                                                      tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                               >  4:152365/1‑139 (MQ=255)
                                                                                                      tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCtt                               >  7:8838/1‑139 (MQ=255)
                                                                                                      tGAGATCATTTTTCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATATTCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTAAAGAGGTTATCTCTCCCGCCAATTTGCtt                               >  1:258553/1‑139 (MQ=255)
                                                                                                              tttttCTTTTTTTGAAAAGCGATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTgg                       <  7:322177/139‑1 (MQ=255)
                                                                                                                                  gATTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGAcc   <  8:280223/139‑1 (MQ=255)
                                                                                                                                  gATTACCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTTTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGAcc   <  2:258553/139‑1 (MQ=255)
                                                                                                                                   aTTCCCGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg  <  3:261927/139‑1 (MQ=255)
                                                                                                                                   aTTCCCGGTCACATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAATACTTTATAGATTTTCAAGAGGTTATCTCTCCCGCCAATTTGCTTTTGACTGGGATTTTACCTCCTTTTGACCg  <  6:160173/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
CAGCTATTTCAATATGCTCCTCCGGCAGTGTTTGCAGGATTTGTTTAAACTTTTCATTCTCGTCGTGCACGACAAAGATCTTTTCCACTTTATTCATAGGGATGAGGTCATTTTTCTTTTTTTGAAAAGCGATTCCTGGTCCCATCACGATTTTTTCCTGATCATCCTCTTTTATTAAAGCCGCATTGTTGTTTAGTACTTTATAGATTTTCAAGAGGCTATCTCTCCCGCCAATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCG  >  CP006881/3724613‑3724882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A