Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F53 I19 R3 125 83.6 3029080 95.4% 2889742 127.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,725,209 +T intergenic (‑41/‑488) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,2051.T77.2% 140.2 / 40.1 59intergenic (‑37/‑492)U712_19205/U712_19210Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand):  ref base . (4/9);  new base T (30/14);  total (36/23)
Fisher's exact test for biased strand distribution p-value = 2.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑C‑T‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATAT  >  CP006881/3725068‑3725337
                                                                                                                                             |                                                                                                                                         
tGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCACT‑CAG‑C‑C‑t                                                                                                                                          >  3:254182/1‑139 (MQ=255)
 gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑C‑t‑t                                                                                                                                        >  7:11222/1‑139 (MQ=255)
 gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑C‑t‑t                                                                                                                                        >  4:288826/1‑139 (MQ=255)
 gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑C‑t‑t                                                                                                                                        >  1:221622/1‑139 (MQ=255)
       aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGG‑CAG‑C‑C‑t‑ttttagc                                                                                                                                  >  4:46258/1‑135 (MQ=255)
       aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CCG‑C‑C‑t‑ttttag                                                                                                                                   >  6:256086/1‑135 (MQ=255)
       aaTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCAGCCACACCCGTAAAGAAACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTGTTTTTGGCAGG‑CAG‑C‑C‑t‑ttttagc                                                                                                                                  >  1:118651/1‑135 (MQ=255)
              cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CCG‑C‑CTT‑TTT‑A‑GCAGCCC‑Gt                                                                                                                         >  7:576/2‑139 (MQ=255)
              cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTTGCAGG‑CAG‑C‑CGT‑TTT‑A‑GCAGCCC‑Gt                                                                                                                         >  6:191498/2‑139 (MQ=255)
              cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑Gt                                                                                                                         >  8:61001/2‑139 (MQ=255)
              cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCAGG‑CAG‑C‑CGT‑GTT‑A‑GCAGCCC‑Gt                                                                                                                         >  4:60465/2‑139 (MQ=255)
              cgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGGAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCGTTTTGGCAGG‑CGG‑C‑C‑TGTTT‑A‑GCAGCCC‑Gt                                                                                                                         >  7:330970/2‑139 (MQ=255)
                cGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑Gttt                                                                                                                       >  1:214753/1‑139 (MQ=255)
                 ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACCCCCGTAAAAATACACGCCACCGTTTCATAGAAAAACATCCCTCCGCTTTTTTTTGGCAGG‑CGC‑C‑C‑TGTTG‑A‑GCAGCCC‑Ggtta                                                                                                                      >  2:183628/1‑138 (MQ=255)
                 ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑Gtttt                                                                                                                      >  2:25390/1‑139 (MQ=255)
                 ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑Gttt                                                                                                                       >  7:265322/1‑138 (MQ=255)
                 ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCGTCTTTTTGGCCGG‑CAG‑C‑C‑TGTTT‑A‑GCCGCCC‑Gtttt                                                                                                                      >  3:197225/1‑139 (MQ=255)
                                tCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGccc                                                                                                       <  8:11222/138‑1 (MQ=255)
                                      cGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGcccgcaaga                                                                                                 >  3:32083/1‑132 (MQ=255)
                                              cccAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGc                                                                                        >  4:163093/1‑139 (MQ=255)
                                                   ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTGTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGAtt                                                                                   >  4:233125/1‑139 (MQ=255)
                                                      atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTATCA‑GCCGCACCCCGCAAGAAAAGACGGCCGAtttat                                                                                >  7:11419/1‑137 (MQ=255)
                                                       tcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGAtttctt                                                                               >  5:143692/1‑139 (MQ=255)
                                                             aGACCATCCACCCCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAATAGACGGCCGAGTTCTTTTCTCg                                                                         >  6:66262/1‑139 (MQ=255)
                                                             aGACCATCCACACCCGTAAAGATACAAGCAAACCTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑AAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTACTCA‑GCCCCAGCCCGCAAGAAAAGACGTCCGAATTATTTTATCg                                                                         >  2:213171/1‑139 (MQ=255)
                                                               cccAACCACCCCCGTAAAGAAACAAGCAACCGTTTCATAGAACACCATCCCTCGGCTTCTTTTTGGCAGG‑CAG‑CGT‑T‑TTT‑C‑GCCGCCC‑GTTTTCTCC‑GCCGCCCCCCTAACAAAACCACGGCCGATTTCTTTTCTCGCa                                                                       <  2:210794/138‑1 (MQ=255)
                                                                ccacccacACCCTTAAACATACAAGCAACCGTTTAATACAAAAACAACCCACCGCTTCTTTTTGGCCGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCC‑GCCGCAGCCCCAAACAAAAGACGGCCGATTTCTTTTCTCGCaa                                                                      <  3:46258/139‑1 (MQ=255)
                                                                           cGTAAACATACAACCAAACGTTTCATACAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑C‑GCCGCCC‑GTTTTCTCA‑GCCGCCGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                           <  3:163093/139‑1 (MQ=255)
                                                                             tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTGTCGGAAACGAGCggggg                                                           >  1:188657/1‑134 (MQ=255)
                                                                             tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                           >  8:226128/1‑137 (MQ=255)
                                                                             tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                           >  7:158552/1‑137 (MQ=255)
                                                                             tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAACACGGCCGATTTCTTTTCTCGCAAACTAGAGGctct                                                          >  8:85165/1‑138 (MQ=255)
                                                                             tAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCCCAGCCCGCAAGAAAAGACGGCCGAATTCTTTTCTCGCAAACGAGCGGcgctg                                                         >  5:260977/1‑138 (MQ=255)
                                                                              aaaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGAGTTCTTTTGTCGCAAACTAGCGGCTCTAg                                                        >  1:300683/1‑139 (MQ=255)
                                                                                ccaTACAAGCAAACGTTTCATACAAAAACATCCCTCCGCTTCTTTTTGGCCGG‑CAG‑C‑CTT‑TTT‑C‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGAAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCa                                                      <  4:344804/137‑1 (MQ=255)
                                                                                ataTACAACCATCCGTTTCATATAAAAACATCCCTCCGCTTCTTTTTGGCAGGTTGG‑C‑T‑T‑TTT‑A‑TCTGCCC‑GTTTTCTCA‑TCCGCACCCCGATAGAAACGACGGCCGATTTCTTTTGTCGCAAAATAGCGGCTCTAGCa                                                      <  6:181095/137‑1 (MQ=255)
                                                                                  aTACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTGGCAAACTAGCGGCTCTAGCaa                                                     >  4:53233/1‑138 (MQ=255)
                                                                                    aCAAGCAAACGTTTCATAGAAAAACATCCCTCCTCTTCTTTTTTGCAGG‑CAGAC‑T‑T‑TTT‑A‑GCATCCC‑GTTTTCTCA‑GACGCAGCCCGCAAAAAAAGACGGCCGATTTGTTTTATAGCAAACTAGCCGCTCTAGCaaaa                                                   >  7:230317/1‑138 (MQ=255)
                                                                                    aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CCG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCC‑GCCGCCGCCCGCAAGAAAAGACGGCCGAGTTCTGTTCTCCCAAACTAGGGGCTCTTGCAAAAc                                                  >  6:358422/1‑139 (MQ=255)
                                                                                    aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCCGCCCGCAAGAAAAGACGGCCGAGTTCGTTTCTCGCAAACTAGCGGCTCTGGCAAAAc                                                  >  8:176547/1‑139 (MQ=255)
                                                                                    aCAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑ATTTTCTCA‑GCCGCATCCCGCAAGAAAACACGGCCGATTTCTTTTCTCGCAAAAATGCCGCTCAAGCAAAAc                                                  >  5:181095/1‑139 (MQ=255)
                                                                                      aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTGTTTTTGGCAGG‑CCG‑C‑CTT‑TTT‑A‑TCAGCCC‑GTTTTCTCC‑GCCGCAGCCCGCAAGAAAAGACGGCCGAAATCAGTTCACGCAAACTAGCGGCTCAAGCAAAACt                                                 >  6:197602/1‑138 (MQ=255)
                                                                                      aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACt                                                 >  1:123784/1‑138 (MQ=255)
                                                                                      aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACt                                                 >  3:249192/1‑138 (MQ=255)
                                                                                        gCAAACGTTTCATATAAAAACAACCCTCCGATTCTTTTGGGCCGG‑CAG‑C‑C‑T‑TTT‑ACGCAGCCC‑GTTTTCTCC‑GCCCCACCCCGAAACAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                              <  6:345956/139‑1 (MQ=255)
                                                                                        ccAACCGTTTCATAGAAAAACAACCCTCCGCTTATTTTTGGCAGC‑CAG‑C‑CTT‑TTT‑C‑GCAGCCG‑GTTTTCTCC‑GCCGCAGCCCGAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                              <  8:164225/138‑1 (MQ=255)
                                                                                        caaaaCGTTCCAAACAAAACCAGCCTCCCGCTTCTTTTTGGCCGC‑CAC‑C‑G‑T‑TTT‑A‑CCCCCCCGGTTTCCTCC‑GCCCCACCCCCAAAGAAACGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAt                                              <  7:366414/137‑1 (MQ=255)
                                                                                        acAACCGTTCCACACAAAACCATCCCTCCGCTTCTTTTGCGCCGG‑CCG‑C‑CTT‑TTT‑C‑GCCGCCC‑GTTATCACC‑CCCCCCCCCCCCAAAAACCCCCGGCCGATTGCTTTCCGCCCAAACTCGCGGCTCTAGCAAAACTCAt                                              <  8:330970/138‑1 (MQ=255)
                                                                                          aaaCGTTCAAAATAAAAACACCCCCCCGCTTCTTTTTGCCTGC‑CCG‑C‑CTT‑TTT‑C‑GCAGCCC‑GTTTGCCCC‑GCCGCCGCCCCCAATAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa                                            <  5:191498/139‑1 (MQ=255)
                                                                                            ccttttCATACAAAAACACCCCTCCGCTCTTTTTGGGCCGG‑CCG‑C‑C‑T‑TTT‑ACCCACCCC‑GTTTTCTCC‑GCCCCCCCCCCCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                          <  8:576/136‑1 (MQ=255)
                                                                                            ccgattCATACAAAAACACCCCTCCGCTTTTTTTTGCCTGG‑CCC‑CAT‑T‑TTT‑A‑CCCGCCC‑GTTTTCTCC‑CCCGCCCCCCCCAACAACACACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                          <  4:32083/135‑1 (MQ=255)
                                                                                            ccGTTTCATACAAAACCACCCCGCCGCTTCTTTTTGCCCGC‑CAG‑C‑C‑T‑TTTAA‑GCAGCCC‑TTTTGCCCC‑CCCGCCCCCCGAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                          <  5:256086/138‑1 (MQ=255)
                                                                                            ccGTTCCATACAAAAAAATCCCTCCGATTCTTTTTGGCCGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGAAAGAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                          <  7:226128/138‑1 (MQ=255)
                                                                                            aCGTTTCATAGAAAACCACCCCTCCGCTTCTTTTTGGCAGG‑CCG‑C‑CTT‑TTT‑C‑CCAGCCC‑GTTTCCCCC‑GCCGCCCCCCGAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                          <  2:214753/139‑1 (MQ=255)
                                                                                            aCGTTTCATACAAAAACATCCCTCCGCTTCTTTTGGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                          <  8:295166/139‑1 (MQ=255)
                                                                                            aCGTTTAATACAAAAACATCCCTCCGCTTCTTTTTGCCAGG‑CAG‑C‑CTT‑TTT‑C‑GCACCCC‑GTTTTCTCC‑CCCCCACCCCGAAACAAACCACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGAct                                          <  7:181449/139‑1 (MQ=255)
                                                                                            aCGTTGAATATAAAAACATCCCGCGGTTTATTTTTGGCAGG‑CCG‑C‑T‑T‑TTTAA‑GCCCCCG‑TTTTTCTCA‑CCCGCCCCCCCCAACAAACCACGGCCGATTTCTTTTCTCGCAAACTCGCGGCTCTAGCAAAACTCATGAct                                          <  2:123784/139‑1 (MQ=255)
                                                                                             cGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGActc                                         >  8:30087/1‑139 (MQ=255)
                                                                                                  aaTAGAAAAACATCCCTCCGCTTCTTTTTGGAAGC‑CAC‑C‑CTT‑TTT‑A‑CCAGACC‑TTTTTCACA‑CCCGCACCCCTCAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATg                                     <  7:313822/137‑1 (MQ=255)
                                                                                                         aaaCATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTATAGCAAAATAGCGGCTATAGCAAAACACATGCCTCTATGAAAGACCg                             >  5:207099/1‑139 (MQ=255)
                                                                                                            cagccccccGCGTGTTTTTGGCTGG‑CAG‑C‑C‑T‑TTT‑T‑ACCACCC‑CTTTTCGCACGCCCCCGCCCCAAATAAACCACGGCCGATTGCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTgg                          <  8:265322/136‑1 (MQ=255)
                                                                                                                    cGCTTCTTTTGGGCCGG‑CCG‑C‑CTT‑TTT‑C‑GCCCCCG‑GTTTGCTCC‑CCCCCAGCCCCAAACAAACGCCGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGaa                  <  6:143692/139‑1 (MQ=255)
                                                                                                                         cttTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCAAGACTCTAAGATAGACCGTGGAAAGAGAATTGtt             >  4:192131/1‑139 (MQ=255)
                                                                                                                         cttTTTGGCAGG‑CAG‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACCGCCGATTTCTTTTCTCGCAAACTAGCCGCACTAGCAAAACTCAAGACTCTATGAGAGACCGTGGAAAGAGAATTcgt             >  7:292294/1‑137 (MQ=255)
                                                                                                                         atttttGCCAGG‑CAG‑C‑CTT‑TTT‑A‑CCCGCCC‑GTTTTCTCA‑GCCCCCGCCCGAAATAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt              <  3:192131/137‑1 (MQ=255)
                                                                                                                                    g‑cag‑C‑CTT‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTGTAGCAAAACTAATGACTCTATGATAGACCGTGGAAAGAGAATTCATTGTCTCAatat  >  4:4649/1‑139 (MQ=255)
                                                                                                                                             |                                                                                                                                         
TGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGG‑CAG‑C‑C‑T‑TTT‑A‑GCAGCCC‑GTTTTCTCA‑GCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATAT  >  CP006881/3725068‑3725337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A