Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A18 F53 I5 R2
|
122 |
92.4 |
3471984 |
95.3% |
3308800 |
125.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
3,724,977 |
T→C |
Q64Q (CAA→CAG) |
U712_19205 ← |
Uncharacterized protein ywcI |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 3,724,977 | 0 | T | C | 92.5%
| 123.3
/ NA
| 43 | Q64Q (CAA→CAG) | U712_19205 | Uncharacterized protein ywcI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (6/31); minor base A (3/0); total (11/32) |
| Fisher's exact test for biased strand distribution p-value = 8.50e-03 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTC > CP006881/3724846‑3725105
|
aTTTGCTTTTGACTGGGATGTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGctttt < 2:273377/139‑1 (MQ=255)
tgCTTTTTACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGcttt < 5:245912/134‑1 (MQ=255)
cTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTcc < 8:225095/139‑1 (MQ=255)
ttttGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTttctt < 3:305405/136‑1 (MQ=255)
cTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCAt < 4:347993/136‑1 (MQ=255)
cTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca < 8:181808/139‑1 (MQ=255)
ttttACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGGTTTTCTTCCTCATTCACAg > 5:192767/1‑136 (MQ=255)
ctcctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATcc < 3:247594/136‑1 (MQ=255)
ctcatTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATGCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGcc < 7:104889/139‑1 (MQ=255)
atcctTTTGACCGCCAAGTAAAGATGCGGGCCCTCTCTCTCCTGCTTTGGCGATTCCTTGAAGTCTTTCAAACATATTCTATTATAAAGCGCGGCTGCTTTCTCTTTCTGCTTTTGTTGCTCATTCACTGCCATcc < 6:3942/135‑1 (MQ=255)
cctTTTGCCCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCTGCGGCTTTCGGATTCAGCTTTTCTTACTAATTCacagccaacagccat > 2:232107/1‑138 (MQ=255)
cctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca < 8:335493/125‑1 (MQ=255)
cctTTTGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTca > 7:335493/1‑125 (MQ=255)
tGACCGCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTACTTCCTCATTCACAGCCATCCGCCATTCAt < 7:95061/138‑1 (MQ=255)
ccccATGAAAACATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTAAAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATCAACCGCCATCCGCAATTCATaa < 6:3924/134‑1 (MQ=255)
cGCCATGTAAAGATGCGGGCCCGCTCTATCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCGATCCGCCATTCATaa < 6:192767/136‑1 (MQ=255)
gCCATGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTAGGGTTTTCTTCCGGATTCACAGCCATCCGCCATTCATAAAAc > 1:48684/1‑138 (MQ=255)
tGTAAAGATGCGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGa < 3:374642/136‑1 (MQ=255)
aaaGATGCGGGCCCGCTCTCTCCTGCTGTGGCGATTCCTTGACGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCGTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAAcga < 2:39817/138‑3 (MQ=255)
cGGGCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTATGCTTTTCTTGCTCATTGACCGCCCTCCGCCATTCATAAAACGAAACGAGTcctgag > 8:229833/1‑135 (MQ=255)
gacaggCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATATAGGGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGc < 7:403301/134‑1 (MQ=255)
gCCCGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTGTGCGTTTCTTCCTGATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCtgcg > 5:425690/1‑136 (MQ=255)
ccgCTCTCTCCTGCTTTGGCGATTCCTTTATGGCTTGCAAACTTCTTATTTTTTAATGCGCGGGGGCTTTCGCTTTCTGCTTTTATACCTCATTCACAGCCATCGGCCATTCATAAAACGAAACGAGTCCTGCGGc < 4:42257/134‑1 (MQ=255)
ccgCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTGGCGTTGTGCGGTTCTTCCTCATTGCCCGCCCGCCGCCCTTCATAAAACGCAACCCGTCCAGCGGc > 3:46887/3‑136 (MQ=255)
ccgCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGGTTTTCTTCCTCATTCAAAGCCATCCGCCATTCATTAAACGAAACGAGTCCTGCGGc > 3:345412/3‑136 (MQ=255)
tcCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCAcagc < 1:129988/137‑1 (MQ=255)
cTGCTTTGGCGATTCCTTGAAGGATTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACagccgcc < 2:299038/139‑2 (MQ=255)
cTTTGGCGATTCCTTGAAGGCTTGCAAACATCAACTTTAATAAAGCGCGGCGGCTTTCGCGTTAATGTTTTATTACTCAATCACACCCCTCCCCCAGTCATAAAACGAAACGAGTCCTGCGGCCCGCAAcaccgcat > 8:403301/1‑135 (MQ=255)
tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCa > 7:349183/1‑93 (MQ=255)
tGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCa < 8:349183/93‑1 (MQ=255)
gCGATTCCTTGAAGGGTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCCTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCctttcttt < 7:416567/138‑1 (MQ=255)
gAAGGCTTGAAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGt < 3:256480/135‑1 (MQ=255)
aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 7:21900/139‑1 (MQ=255)
aaGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCCTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 8:43493/139‑1 (MQ=255)
cgCTTGAAACCTTCTTCTTTATTAACGGGGGGCGGCTTTGGATTTGTGCTTTGCTTCCTCATTCCCCCCCACCCCCATTTCAT‑AAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAg < 1:101369/138‑1 (MQ=255)
gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCt < 5:61767/136‑1 (MQ=255)
gCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGa < 2:69459/138‑1 (MQ=255)
cAAACAGCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGcc < 5:359578/136‑1 (MQ=255)
tcttTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGcc > 8:55538/1‑55 (MQ=255)
tcttTTATAAAGCGCGGCGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGcc < 7:55538/55‑1 (MQ=255)
taACGCGCGGGGGCTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCTTCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACTGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAg < 5:435830/136‑1 (MQ=255)
cTTTCGCTTTCTGCTTTTCTTCCTCATTCACAGCCATCCGCCATTCATAAAACGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTc < 2:48684/139‑1 (MQ=255)
cTTTCGCTTTCTGCTTTTCTTCCTCAATCACAGCCATCCGCCATTCATAAACCGAAACGAGTCCTGCGGCCTGCACAGCCGCCTTTCTTTGGTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCt < 5:257692/136‑1 (MQ=255)
|
ATTAGCTTTTGACTGGGATTTTACCTCCTTTTGACCGCCATGTAAAGATGCGGGCCAGCTCTCTCCTGCTTTGGCGATTCCTTGAAGGCTTGCAAACATCTTCTTTTATAAAGCGCGGCGGCTTTCGCTTTTTGTTTTTCTTCCTCATTCACAGCCATCCGCCACTCATAAAACGAAACAAGTCCCGCGGCCTGCACCGCCGCTTTTCTTTGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTC > CP006881/3724846‑3725105
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
