Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F56 I20 R2
|
1958 |
92.1 |
3575798 |
91.1% |
3257551 |
126.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
2,173,870 |
T→C |
L352L (TTA→TTG) |
U712_10875 ← |
CCA‑adding enzyme |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 2,173,870 | 0 | T | C | 98.2%
| 183.8
/ NA
| 57 | L352L (TTA→TTG) | U712_10875 | CCA‑adding enzyme |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (32/24); minor base A (1/0); total (33/24) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTTCCTGTCACAACAGCCTGCTCGATCCGCTGTAATTCTTCCGACACCCATTTTCCGGCAGGCCGGTTACGAAGCGCTAGTAAATCTTTACCCGTGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCT > CP006881/2173735‑2173999
|
aTGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCgcaagcaag < 8:395223/139‑2 (MQ=255)
aCCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCgcaagcaa < 4:22719/132‑1 (MQ=255)
aCCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCgcaagcaa > 3:22719/1‑132 (MQ=255)
aCCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTa > 6:293976/1‑139 (MQ=255)
aCCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTa > 2:268091/1‑139 (MQ=255)
aCCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTa > 3:422298/1‑139 (MQ=255)
aTGTCTTCAGCCACTCCTCTATGTTTTTCTTTTGATTGGAGCGCTTCCCTGTCGCAACCGCCTGCTCGATCCACTGGAATTCTTCTGACCCCACTATTCCGGCCGGCCGGTTTCGCAGCGCAAGAACGTCATTACCCGt > 4:134381/1‑139 (MQ=255)
gCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAg < 1:376655/139‑1 (MQ=255)
gCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAg < 8:110915/139‑1 (MQ=255)
gCCACTCCTATATGTGTTTCTTTTGATTGGAGATCTTCCCTGTCAAAACAGCCTGCTCTATCCACTGTAATTCTTCTGACACCCATTTTCCGGACGGCCGGTTTCTAAGCGCAAGCAAGTACTTACGCGTGATATCatg < 6:365691/139‑3 (MQ=255)
aCTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTGTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATc > 1:302637/1‑139 (MQ=255)
ctcctcTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATcc > 2:271651/1‑139 (MQ=255)
tcctcTATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGACCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATcct > 4:312202/1‑138 (MQ=255)
tATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTTGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAgg > 4:422123/1‑139 (MQ=255)
tATGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAgg > 6:440005/1‑139 (MQ=255)
aTGTGTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGCCACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGc > 8:385660/1‑139 (MQ=255)
tgtgTTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCt > 7:182370/1‑139 (MQ=255)
gtTTCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCtttt > 3:412317/1‑139 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTcc < 7:318945/105‑2 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTcc > 8:318945/1‑104 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTg < 7:94477/114‑1 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTg > 5:238132/1‑114 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTg < 6:238132/114‑1 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTg > 8:94477/1‑114 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGa > 5:204147/1‑139 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGa > 8:253740/1‑139 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGa > 7:140487/1‑139 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATAAAAAGATCCTTAAGGCTTTTGa > 3:179756/1‑139 (MQ=255)
ttCTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTGTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCGTAAGGCTTTTGa > 6:11784/1‑139 (MQ=255)
cTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAg < 4:58211/120‑1 (MQ=255)
cTTTTGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAg > 3:58211/1‑120 (MQ=255)
ttttGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAATCAAGTCCGTACACGTGATATCAAGATCCTTAAGGCTTTTGATCg > 8:434399/1‑139 (MQ=255)
ttGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCgg > 3:199180/1‑138 (MQ=255)
tGATTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGc < 1:429698/138‑1 (MQ=255)
aTTGGAGAGCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTATCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAgg < 7:95464/139‑1 (MQ=255)
gagCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGa < 8:140487/139‑1 (MQ=255)
gagCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGa < 7:151193/139‑1 (MQ=255)
agCTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGAt < 1:271651/139‑1 (MQ=255)
cTTCCCTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATaa < 7:385660/139‑1 (MQ=255)
cccTGTCACAACAGCCTGCTCTATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAg < 7:434399/136‑1 (MQ=255)
ccTGTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAgcg < 5:293976/138‑3 (MQ=255)
gTCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAgcgtact < 1:86079/139‑7 (MQ=255)
tCACAACAGCCTGCTCGATCCCCTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTTCCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCTGGTGTTGAGAAgcgtact > 1:415330/1‑132 (MQ=255)
tCACAACAGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAgcgtact < 3:312202/138‑7 (MQ=255)
acaacaGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGa < 6:431444/126‑1 (MQ=255)
acaacaGCCTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGa > 5:431444/1‑126 (MQ=255)
ccTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCtt < 1:268091/139‑1 (MQ=255)
ccTGCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCtt < 4:199180/139‑1 (MQ=255)
gCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAg > 2:18396/1‑139 (MQ=255)
gCTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAg > 1:306096/1‑139 (MQ=255)
cTCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGt < 5:440005/139‑1 (MQ=255)
tCGATCCACTGTAATTCTTCTGACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGtt > 8:132528/1‑139 (MQ=255)
ctgACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTc > 7:392584/3‑139 (MQ=255)
ctgACACCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTc > 6:198396/3‑139 (MQ=255)
acacCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAgcgt > 5:88301/1‑96 (MQ=255)
acacCCATTTTCCGGCCGGCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAgcgt < 6:88301/99‑4 (MQ=255)
gCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGatat > 7:308515/1‑139 (MQ=255)
gCCGGTTTCGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGatat > 6:253246/1‑139 (MQ=255)
tttcGAAGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTgg < 4:412317/136‑1 (MQ=255)
aaGCGCAAGCAAGTCCTTACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCt < 7:253740/139‑1 (MQ=255)
|
ATGTTGACCGCATGTCTTCAGCCACTCCTCTATGTGTTTCTTTTGATTGGAGAGCTTTCCTGTCACAACAGCCTGCTCGATCCGCTGTAATTCTTCCGACACCCATTTTCCGGCAGGCCGGTTACGAAGCGCTAGTAAATCTTTACCCGTGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCT > CP006881/2173735‑2173999
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
