Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F58 I1 R3
|
118 |
63.0 |
2369054 |
95.4% |
2260077 |
127.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
CP006881 |
1,653,878 |
A→G |
Y241C (TAT→TGT) |
U712_08495 → |
GTP‑sensing transcriptional pleiotropic repressor CodY |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP006881 | 1,653,878 | 0 | A | G | 97.9%
| 166.4
/ NA
| 47 | Y241C (TAT→TGT) | U712_08495 | GTP‑sensing transcriptional pleiotropic repressor CodY |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (25/21); minor base C (1/0); total (26/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTG > CP006881/1653745‑1654008
|
aaTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCa < 1:197591/139‑1 (MQ=255)
aTGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATc > 7:185614/1‑137 (MQ=255)
aaGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAgg > 7:157928/1‑138 (MQ=255)
aGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTa < 1:207096/139‑1 (MQ=255)
ggTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTAc > 3:164699/1‑139 (MQ=255)
cttcttGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTa > 7:136624/1‑138 (MQ=255)
ttcttGTTGCAAGTAAACTTGCTGCCCGTGTCGGCATTAACCGTTCTGTTATTGTGAACGCACTCAGAAGGCTGGAGCGAGGCGGTGTTATCGATTCTAGATCATTAGGAATGAAAGGTAGTTGTATGATGTTACTtaa > 6:195149/1‑139 (MQ=255)
cttGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTATATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAaac > 8:239996/1‑138 (MQ=255)
gCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAAt > 8:113386/1‑139 (MQ=255)
aGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCAATCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTc > 6:46332/1‑138 (MQ=255)
aCCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGa < 4:164699/139‑1 (MQ=255)
aCCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGaaaatctaaaatct > 2:134595/1‑138 (MQ=255)
ccGTTCTGTTATTGTGAACGCACTCAGACAGCTGGAGAGCGCCGGTGGTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCAt < 2:151635/139‑1 (MQ=255)
tCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAAt > 7:175406/1‑139 (MQ=255)
tGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGACTGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATca < 8:92020/139‑1 (MQ=255)
ttATTGTGAACGCACTCAGATATCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAAGAAATTCCTAATTGAATTTGAAAATCTAAAATCTCATTAATcac < 6:78206/138‑1 (MQ=255)
ttATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATca < 8:185614/137‑1 (MQ=255)
ttATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATc < 8:136624/136‑1 (MQ=255)
gtgAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACaaa > 6:106755/1‑136 (MQ=255)
gtcaaCGCACTCAGAAAGCTGGAGAGCGCAGGTGTTATCGAGTCTAGATCATTAGGAATTAAAGGTACTTCTATCAAGGTACAACACAACAAATTCATAATTGAATTAGAAAATATAAAATCTCATTAATCAaaaa > 5:78206/4‑136 (MQ=255)
tgAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAg < 7:239996/138‑1 (MQ=255)
aaCGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGaa < 2:40690/138‑1 (MQ=255)
aaCGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGaa < 2:238457/138‑1 (MQ=255)
aaCGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGaa < 2:202844/138‑1 (MQ=255)
aaCGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAAc > 7:146845/1‑139 (MQ=255)
ggAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTaa < 1:152029/80‑1 (MQ=255)
ggAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTaa > 2:152029/1‑80 (MQ=255)
ggAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCtt > 5:29700/1‑139 (MQ=255)
ggAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCtt > 3:153340/1‑139 (MQ=255)
agagCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCtttt > 7:129220/1‑139 (MQ=255)
ccGGAGGTATCGAGTCTAGATCATTACGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTAtt < 7:230900/139‑1 (MQ=255)
tCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGaaa > 1:138728/1‑139 (MQ=255)
tCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGaaa > 3:149792/1‑139 (MQ=255)
tCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGaaa > 4:224633/1‑139 (MQ=255)
tCTAGATCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGaa > 4:108581/1‑138 (MQ=255)
tCATTAGGAATGAAATGTACTTGTATCAATGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAAt < 6:63409/138‑1 (MQ=255)
tCATTAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATa < 6:86041/139‑1 (MQ=255)
ttAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGc > 2:187231/1‑139 (MQ=255)
tAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCttttt > 2:219871/1‑97 (MQ=255)
tAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCttttt < 1:219871/97‑1 (MQ=255)
tAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATaa < 5:132472/123‑1 (MQ=255)
tAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATaa > 6:132472/1‑123 (MQ=255)
tAGGAATGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCt > 3:268530/1‑139 (MQ=255)
tGAAAGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTAttt > 4:286788/1‑139 (MQ=255)
aaGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTcc < 4:149792/138‑1 (MQ=255)
aaGGTACTTGTATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTcc < 5:46332/138‑1 (MQ=255)
atgTACTTGTATGAATGTACTAAACAACAAATTACTAATTTAATTAGAAAATCTAAAATCTCATTAATCAAAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTg < 8:48037/137‑1 (MQ=255)
|
AATGAAGGTCTTCTTGTTGCAAGTAAAATTGCTGACCGTGTCGGCATTACCCGTTCTGTTATTGTGAACGCACTCAGAAAGCTGGAGAGCGCCGGTGTTATCGAGTCTAGATCATTAGGAATGAAAGGTACTTATATCAAGGTACTAAACAACAAATTCCTAATTGAATTAGAAAATCTAAAATCTCATTAATCACAAAAAGAACCCTTTTTGAGGGTTCTTTTTTTATTTCAAATAAAGGAAATCAATAAGCTTTATTTCCTG > CP006881/1653745‑1654008
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
