Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F44 I1 R1	27	48.3	2078744	90.6%	1883342	142.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,026,927	A→T	V30E (GTG→GAG)	mgsA ←	methylglyoxal synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,026,927	0	A	T	94.4%	150.9 / NA	55	V30E (GTG→GAG)	mgsA	methylglyoxal synthase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (28/23); minor base G (0/3); total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGC  >  NC_000913/1026789‑1027066

gcgcCAACCTGCTGGTCACCCCCCAGTGGGCCACTCAACACCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTACCGGTTGATGCCGTTCCTCCCa                                                                                                                                         <  1:69286/143‑1 (MQ=255)

gcgcCAACCGGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCa                                                                                                                                         <  1:310615/143‑1 (MQ=255)

 cgcCAGCCTGTTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAg                                                                                                                                        <  2:427559/143‑1 (MQ=255)

       ccTGCTGGTCACCCCCCATTGGGCCACGCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCtcatc                                                                                                                                  <  1:282662/143‑1 (MQ=255)

              ggcACCCCCCATTGGGGCGCTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCAttt                                                                                                                           <  2:40294/141‑1 (MQ=255)

                   cccccATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGCAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTTCTCCCGGCTCCTCAGCAtttgtttg                                                                                                                      >  1:973341/1‑143 (MQ=255)

                     cgccttGGGCCACTCAACATCGCGTTGACGTTCATGCTGGTCGCGCGGGAAATTAAGTTACCGGTAGTGTTTGTTGCATACAGGACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCa                                                                                                                    <  2:986722/139‑1 (MQ=255)

                     cccATTGGGCCACTCACCACCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCa                                                                                                                    <  1:64637/143‑1 (MQ=255)

                     cccATGGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCa                                                                                                                    <  1:331032/143‑1 (MQ=255)

                        aTTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTg                                                                                                                 >  1:387944/1‑143 (MQ=255)

                              ccACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCgtg                                                                                                           <  2:811513/143‑1 (MQ=255)

                               cACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGAGCgtgt                                                                                                          >  1:27474/1‑143 (MQ=255)

                               cACGCATCATCGCGTTGACGTTCAGGCCGGTCGGGCGCGACACTACGTTACCGGTAGTCCCTGGTGCATACAGTACTTGTTGTTCCAGTAACTTTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCgtgt                                                                                                          <  1:264421/143‑1 (MQ=255)

                                  tCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGcc                                                                                                       >  1:263922/1‑143 (MQ=255)

                                                   ttCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCTAGTAGCGGTTGATGCCGCTGCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCACTATGttt                                                                                      >  2:413577/1‑143 (MQ=255)

                                                     cgcgcgggTCGCGCGGGACATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTcc                                                                                    <  2:997368/137‑1 (MQ=255)

                                                      aTGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCcg                                                                                   <  1:151446/143‑1 (MQ=255)

                                                        gCCGGTCGCGCGGGAAATTAAGTTGTCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCTAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCcgcg                                                                                 <  2:890459/143‑1 (MQ=255)

                                                           ggTCGCGCGGGAAATGAAGGGACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAg                                                                              <  1:733516/143‑1 (MQ=255)

                                                               gcgcgGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGaaa                                                                          >  2:591985/1‑143 (MQ=255)

                                                                 gcgGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCCTGTGCCACCAGCGCAACATGTTTTCGCGCAGGCaagg                                                                        >  2:722375/1‑141 (MQ=255)

                                                                 gcgGGAAATTAAGTTACCGGTAATGCATGTTCCATATAGTACGTGTAGTTCCAGTAACGGTTGATGCCGTTCTTCCCAGCGCAATTGTAGATGTATTCAGTTAGCGTGTTCGACTAGCTCAAGAGTTTTCCGCGCAAGTAAAg                                                                        >  2:786888/1‑143 (MQ=255)

                                                                   gggAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCAGTTTCTCCCAGCTCATCAGCATTTGTTTGCCGTGATCGTGTTCCACCGGCGCAATATGGTTCCGCGCAGGTCAAGTg                                                                      >  1:57731/1‑143 (MQ=255)

                                                                       aaTTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCCATATGTTTCCGCGCAGGTAAAGTGCGAg                                                                  >  2:944196/1‑143 (MQ=255)

                                                                       aaTTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAg                                                                  >  2:983104/1‑143 (MQ=255)

                                                                        aTTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATATGTTTGCAGTGATCGTGTACCACAAATGCAATATGTTTCTGCGCTGGTACAGTGCGCgt                                                                 >  2:87167/1‑143 (MQ=255)

                                                                          tAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCACTCTGTTTCCGCGCAGGTTAtgtgtttgtcg                                                               >  1:361560/1‑136 (MQ=255)

                                                                            aGTTCCCGGTAGTGCCTGTGGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAgtcgtc                                                             <  2:853404/143‑1 (MQ=255)

                                                                            aGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAgtcgtc                                                             >  1:163440/1‑143 (MQ=255)

                                                                             gTTCCCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCa                                                            <  2:428915/143‑1 (MQ=255)

                                                                              ttACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGGCGTCAg                                                           >  2:82760/1‑143 (MQ=255)

                                                                                aCCGGTAGTGCCTGTTGCATACAGTACGTGTAGTGTGAATAACGGTGGATGCCGTTCCGCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGtt                                                         <  1:190060/143‑1 (MQ=255)

                                                                                  cGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTGAAGTGCGAGTCGGCAGTTcc                                                       >  1:360516/1‑143 (MQ=255)

                                                                                    gTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGCTCCAt                                                     >  1:672642/1‑143 (MQ=255)

                                                                                     tAGGGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCGGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATa                                                    <  2:621342/143‑1 (MQ=255)

                                                                                       gTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCCGCTCGTCAGCATTTGTTTGCAGTGAGCGTGTGCCCCCCGCGCAATATGTTTCTCCGCACGTACCGTGCGAGTCGTCGGTGCcatcat                                                  >  2:753874/1‑143 (MQ=255)

                                                                                          ccTGTTGCATACAGTACGTGTTGTTCCAGTGTCGGGTGATTCCGTTCCCCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTa                                               <  1:431427/143‑1 (MQ=255)

                                                                                           cTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCCGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTAc                                              >  2:960490/1‑143 (MQ=255)

                                                                                                 cATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGt                                        >  1:1032457/1‑143 (MQ=255)

                                                                                                  aTACAGTACGTGTTGTTCCAGTACCGGTTGATGCCCTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTa                                       <  1:436422/143‑1 (MQ=255)

                                                                                                  aTACAGTACCTTTTGTTCCACTAACGGTTGAGGCCGTTCCTCCCAGCGCATCAGCATTCGTTTGCAGTTATCGGGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTa                                       <  2:915162/143‑1 (MQ=255)

                                                                                                     cAGTACGTGTTGTTCCAGTAACGGTTGATGCCGGTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGtt                                    <  1:153846/143‑1 (MQ=255)

                                                                                                       gTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTaa                                  >  1:591472/1‑143 (MQ=255)

                                                                                                            ggttgttCCAGTAACGGTTGATGCCGTGCCGCCCAGCTCGGCAGCATTTGTTTGCGGTGGTTTTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTc                             <  2:621104/142‑1 (MQ=255)

                                                                                                             gttgttCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCACTATGTTTCCGAGCAGGTAAAGTGTGCGTCGTCAGTTCCATAGTGTACATCGGTAGCTAACTTTca                            >  2:893930/1‑142 (MQ=255)

                                                                                                               tgttCCAGTAACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTa                          >  1:675709/1‑143 (MQ=255)

                                                                                                               tcgtCCCGTTACGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGGGTGCCACCAGCGCAATATGTGTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTa                          <  2:194777/140‑1 (MQ=255)

                                                                                                                    cAGTAACGGTTGATGCCATTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACGGAt                     >  1:120988/1‑143 (MQ=255)

                                                                                                                        aaCTGTTGATTCCGTTCCTCCCAGCTCAACAGCAATAGAGTGCAGTGAGCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACt                 <  1:161824/143‑1 (MQ=255)

                                                                                                                        aaCGGTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACt                 >  2:102185/1‑143 (MQ=255)

                                                                                                                        aaCGGTTCAGGCCGTCCCTCCCAGCTCATCAGCATCTGTTTGCAGTGATCGTGTGCCACCAGCGCAATCTGTGCCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACt                 <  1:540412/143‑1 (MQ=255)

                                                                                                                         aCGGTTGATGCCGTCCCGCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTg                <  1:964526/143‑1 (MQ=255)

                                                                                                                            gTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTaa             >  1:907433/1‑143 (MQ=255)

                                                                                                                            gTTGATGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTaa             >  1:590344/1‑143 (MQ=255)

                                                                                                                                aTGCCGTTCCTCCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCAc         >  1:217608/1‑143 (MQ=255)

                                                                                                                                       tcctccCAGCTCATCAGCATTTGTTTGCAGTGATAGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGCGTCGTCAGTTCCATAATGTACAGCCGTAGTTAACTTGCCTACAGATTCCTGTAAGCACTTATCGc  >  2:65987/1‑143 (MQ=255)

GCGCCAACCTGCTGGTCACCCCCCATTGGGCCACTCAACATCGCGTTGACGTTCATGCCGGTCGCGCGGGAAATTAAGTTACCGGTAGTGCCTGTTGCATACAGTACGTGTTGTTCCAGTAACGGTTGATGCCGTTCCACCCAGCTCATCAGCATTTGTTTGCAGTGATCGTGTGCCACCAGCGCAATATGTTTCCGCGCAGGTAAAGTGCGAGTCGTCAGTTCCATAATGTACATCCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGC  >  NC_000913/1026789‑1027066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A