Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A9 F77 I1 R1
|
25 |
36.2 |
1670822 |
86.2% |
1440248 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,196,232 |
C→T |
T370T (ACC→ACT) |
icd → |
isocitrate dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,196,232 | 0 | C | T | 100.0%
| 48.9
/ NA
| 24 | T370T (ACC→ACT) | icd | isocitrate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/11); total (13/11) |
CCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA > NC_000913/1196092‑1196315
|
ccTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACTCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGa < 1:745451/143‑1 (MQ=255)
gTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCCCCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTGAATCCTGGCTCTATTATTCCCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCg < 2:637375/143‑1 (MQ=255)
aaaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTg < 1:213542/143‑1 (MQ=255)
gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAGAGTAAATCCTGGCTCTATTATTCTCTACGATGAGATGAAGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAcctgata > 1:98943/1‑137 (MQ=38)
aagtcgcCCTGTTTGAAGACACTTACGGTACTGCGCCGAAATATGCCGCTCAGGACAATGTAAATCCTTTCTCTTTTATTCTCTCCGCTGAGATGCTTCTGCGCCATATGGGTTTGACTGAAGCGGTTGATCTGATTGTTaaa < 2:465127/139‑1 (MQ=255)
aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa < 1:727946/143‑1 (MQ=38)
cgcCCGGTGTGGAGCCACCCACGGTACTGCGGCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa < 2:593598/143‑1 (MQ=25)
ggccTGTGTGCAGCCAACCACGGTACAGCGCCGAAATATGCCGGTCAGGGCAAAGTAAATCCTGGCTCTATAATTCTTTCCGCGGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTAt < 2:713344/141‑1 (MQ=21)
gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGAACTGATTGTTAAAGGTAt > 2:339431/1‑143 (MQ=38)
ccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTACACCATATGAGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg > 1:286419/1‑143 (MQ=37)
cTGTTTGAAGCCACCCACGGTATTTGCCCTAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTTAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTTATTGTTAAAGGTATGGa < 2:177607/143‑1 (MQ=25)
tttGAAGCCACCCACGTTACTGCGCCGAAATATGCCGGTCAGGATAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGAAGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg < 1:675436/143‑1 (MQ=25)
ccacccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATATGGGTTGGACTGTATCGGCTGATCTGATTGTTAAAGGTATGAAAGCCGCAATc > 1:113098/1‑143 (MQ=25)
cccGGTACTGCGCCGAAATTTGGCGGTTAGTAGAAAGTTAATTCGGGCTCTATTATTCTCTCCGCTGAGATGATGATGCGCCATATGGGTTGTACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc < 2:539175/141‑1 (MQ=17)
cacGGTACTGCGCCATAATACGCCGGTCAGGACAAAGTAACTCCTGGCTCTATTATTCGCTCCGCTGATATGATGTTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc < 2:403547/143‑1 (MQ=18)
cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCTAAACGTTTCTAACTTAAGCGGCTGACCTGATTGTTAAAGGAATGGAAGGCGCAATCAATGCCAAGAGCGTa > 1:257901/1‑143 (MQ=18)
cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCCTATGGGTTGGACTGAAGCGGCTGACATGAATGATAAAGATATAGAAGGCGCAATCAATGCCAAGACCGTa > 1:379915/1‑143 (MQ=21)
aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAActta > 1:14311/1‑140 (MQ=17)
aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATCCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGACGCGGATGACCTGATTGTTAAAAGTATGGAAGGCGCAATAAAAGCCTACACCGTCACCTa > 2:488971/1‑143 (MQ=18)
tGCTGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGCTGCTGCGCCATATGGGTTGGCCTGAAGCGGCTGACCTGATCGTTAAAGGTATGGAAGGCGCAAACAATGCCAAGACCGTAACATATGAca > 1:531379/1‑142 (MQ=17)
tGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCAGACATAACTGCCTAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt > 2:353225/1‑143 (MQ=18)
ccGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAAGCGCAATCAATGCCAAGACCGTAACTTATGACTTc > 1:665817/1‑143 (MQ=17)
gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt > 2:227623/1‑143 (MQ=14)
gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGACATATGGGTTGGAGTGAAGCAGATGACATGATTGATAAAGGTATGGAAGGAGAAATAAATGCAAAAACCGTAACTTATGACTTCGAACGTATgatgga > 1:229466/1‑143 (MQ=14)
|
CCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGA > NC_000913/1196092‑1196315
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
