Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A23 F66 I1 R1
|
33 |
61.7 |
2789148 |
95.4% |
2660847 |
127.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
BS168_reference_genome |
1,474,890 |
C→T |
L45L (TTG→TTA) |
kre ← |
regulator of transcription factor ComK function via modulation of mRNA stability |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | BS168_reference_genome | 1,474,890 | 0 | C | T | 98.1%
| 181.6
/ ‑3.8
| 53 | L45L (TTG→TTA) | kre | regulator of transcription factor ComK function via modulation of mRNA stability |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (22/30); total (22/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 |
TTGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCAT > BS168_reference_genome/1474763‑1475024
|
ttGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTGTGCTGAACCTTGGATTTATTGAAAAATGAAGCCCTAGTTTCTTTCCTTTACATTCATTGACTACGTTTTGTAAAGCCAGttt > 1:178344/1‑139 (MQ=255)
ggAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGttttt > 8:159131/1‑139 (MQ=255)
gaaTGTGAAAATAGTAGTCTCCGATTTATATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag < 3:13165/137‑1 (MQ=255)
gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag < 4:84932/139‑1 (MQ=255)
gCATGTGAAAATAGTAGGCTCCGATTGAGATAAGCACGTCCGATGGCGGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag < 2:105260/139‑1 (MQ=255)
cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc < 2:246650/139‑1 (MQ=255)
cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTcagc < 7:64177/139‑1 (MQ=255)
aatctcgtagtCTCCGATTGAGATAAGCACGTGCGATTGCTGGTGGCGGAACCGTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGata < 2:178344/132‑1 (MQ=255)
aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata < 5:125734/139‑1 (MQ=255)
aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata < 4:336451/139‑1 (MQ=255)
aaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg < 3:244304/139‑1 (MQ=255)
aaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg < 4:289408/139‑1 (MQ=255)
gtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTa < 7:281039/139‑1 (MQ=255)
agaTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGc > 7:263177/1‑139 (MQ=255)
cACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTGCAGCAGATATAGGTATTTAGGGTTGGGGGCGGttttt > 3:123843/1‑139 (MQ=255)
gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAATCCCTATTTTCTTTCCTTTACCTTATTTGACACGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGTGCGGTTTTTGCa > 6:162414/1‑139 (MQ=255)
gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc > 7:307014/1‑103 (MQ=255)
gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc < 8:307014/103‑1 (MQ=255)
gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata < 2:323405/110‑1 (MQ=255)
gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata > 1:323405/1‑110 (MQ=255)
gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa < 2:7096/139‑1 (MQ=255)
gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa > 6:254631/1‑139 (MQ=255)
gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa > 5:324251/1‑139 (MQ=255)
tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata > 8:137115/1‑109 (MQ=255)
tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata < 7:137115/109‑1 (MQ=255)
tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGcct > 8:310109/1‑137 (MQ=255)
agagtttGATGTTGTCTTAACATTGTATTTTTTGAAAATTTTAGCACTATTTTCTTTCGTTTACTTTCTTTGACAAGCTTTTGTAAATTCCTTTTTTTCTTCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa < 6:124187/133‑1 (MQ=255)
ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcaca < 7:157635/139‑1 (MQ=255)
ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac < 6:196524/138‑1 (MQ=255)
ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca < 6:113188/137‑1 (MQ=255)
ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTGTTGTAAAGCCCGTTTTTTCTGCAGATATAGGTATTTAGGGTTGGGGGCGGTGTTTGCATGGCGGTTca > 4:326578/1‑137 (MQ=255)
ggCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg < 8:105046/127‑1 (MQ=255)
ggCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg > 7:105046/1‑127 (MQ=255)
ggCTGAAACTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTATACTTTACCTTCTTTCACAATATTTAGTAAATCCAGTTTTTTCAGCAGATATAGGTATTTAGGGTTTGGGGCGGTTTTTGCATGGAGGTTcacgtg > 5:124187/1‑136 (MQ=255)
aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGTTTGGTGGCGGTTTTTGCATTGCGGTTCACAGTGTaa > 2:162061/1‑138 (MQ=255)
aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg > 1:305664/1‑122 (MQ=255)
aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg < 2:305664/122‑1 (MQ=255)
aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac > 5:106401/1‑131 (MQ=255)
aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac < 6:106401/131‑1 (MQ=255)
aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTc < 3:295859/129‑1 (MQ=255)
aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTc > 4:295859/1‑129 (MQ=255)
tctttcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCa < 1:162061/138‑1 (MQ=255)
tcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGAt < 7:310109/137‑1 (MQ=255)
tcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCt > 6:326892/1‑139 (MQ=255)
tttACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTc > 3:203275/1‑139 (MQ=255)
tACCTTCTTTGACAAGCTTTTGTAAAGCGCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCg < 7:6220/138‑1 (MQ=255)
tcttTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt > 1:74315/1‑130 (MQ=255)
tcttTGACAAGCTTTTGTAAAGACCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt < 2:74315/130‑1 (MQ=255)
tttGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCa > 2:210008/1‑139 (MQ=255)
aCAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGGATTTAGGGTTGGGGGCGGGTTTTGCATGGCGGTTCACAGTGTAACCTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATgg < 7:303942/138‑1 (MQ=255)
aaGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGGTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtcg < 6:324251/139‑1 (MQ=255)
ttGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCat < 8:263177/138‑1 (MQ=255)
ttGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCat < 1:210008/138‑1 (MQ=255)
|
TTGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCAT > BS168_reference_genome/1474763‑1475024
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
