Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F66 I1 R1 35 99.0 4347662 95.6% 4156364 127.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 1,474,890 C→T L45L (TTG→TTA)  kre ← regulator of transcription factor ComK function via modulation of mRNA stability

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome1,474,8900CT94.7% 310.3 / 3.9 96L45L (TTG→TTA) kreregulator of transcription factor ComK function via modulation of mRNA stability
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (44/46);  total (47/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAGTTGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCAT  >  BS168_reference_genome/1474760‑1475024
                                                                                                                                  |                                                                                                                                      
tAGTTGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGccc                                                                                                                                 <  6:240505/138‑1 (MQ=255)
   ttGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAATCTTTTGTAAAGCCCGttt                                                                                                                             >  5:333947/1‑139 (MQ=255)
      gAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGtttttt                                                                                                                          <  7:343484/139‑1 (MQ=255)
       aGGCATGTGAAATTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAAGCTTGGATTTTTTGATAAATGAAGCCCTATTTTCTTTCCTTGGCATTCTTTGAGAAGCTTTTGTATAGCCCGtgtttt                                                                                                                          >  4:472287/1‑138 (MQ=255)
       aGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATATTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGtttttt                                                                                                                          >  2:74874/1‑138 (MQ=255)
        ggCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCACTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCAGtttttt                                                                                                                          >  6:87284/1‑137 (MQ=255)
         gCATGTGAAAATAGTAGTCTCCGATTTAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       <  5:68890/139‑1 (MQ=255)
         gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       <  4:366315/139‑1 (MQ=255)
         gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       <  5:508738/139‑1 (MQ=255)
         gCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       <  4:191909/139‑1 (MQ=255)
          cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                      <  3:251626/139‑1 (MQ=255)
          cATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcagc                                                                                                                      <  4:170519/139‑1 (MQ=255)
            tgtgAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       <  6:380395/136‑1 (MQ=255)
            tgtgAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       >  5:380395/1‑136 (MQ=255)
                 aaaTAGTAGTCTCCGATTGAGATAAGGTCGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCGCGTTTTTTCAGCAGAtata                                                                                                               <  5:509748/139‑1 (MQ=255)
                 aaaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGatat                                                                                                                <  5:494124/138‑1 (MQ=255)
                  aaTAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg                                                                                                              <  4:91284/139‑1 (MQ=255)
                        agtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAttt                                                                                                        >  3:91255/1‑139 (MQ=255)
                        agtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAttt                                                                                                        >  3:50792/1‑139 (MQ=255)
                         gtCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTAttt                                                                                                        <  4:50792/138‑1 (MQ=255)
                          tctcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAATAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTa                                                                                                       <  6:531503/138‑1 (MQ=255)
                            tcCGATTGAGATAAGCACGTCCGTTTGCTGTTGGCTGAACCTTGGATTTTTTGAATAATGAAGCCCTATTTTCTTTCCTTTGCCTTCTTTGACATGCTTTTGTAAAGGCGGTTTTTTCAGCTGATATTGGTATTTAggg                                                                                                    >  8:274538/1‑139 (MQ=255)
                            tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                               >  8:255642/1‑128 (MQ=255)
                            tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                               <  7:255642/128‑1 (MQ=255)
                            tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAggg                                                                                                    >  6:285845/1‑139 (MQ=255)
                            tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCATTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       <  8:215932/120‑1 (MQ=255)
                            tcCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCATTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       >  7:215932/1‑120 (MQ=255)
                                    agaTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGatat                                                                                                                >  8:19610/1‑119 (MQ=255)
                                    agaTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGatat                                                                                                                <  7:19610/119‑1 (MQ=255)
                                    agaTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTggggg                                                                                             <  8:149434/138‑1 (MQ=255)
                                      aTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg                                                                                                              <  2:37467/119‑1 (MQ=255)
                                      aTAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg                                                                                                              >  1:37467/1‑119 (MQ=255)
                                        aaGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGtt                                                                                        >  1:275089/1‑139 (MQ=255)
                                         aGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGCAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGttt                                                                                       >  4:511361/1‑139 (MQ=255)
                                              gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                                  >  3:456509/1‑139 (MQ=255)
                                              gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                                  >  8:527034/1‑139 (MQ=255)
                                              gTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCa                                                                                  >  8:538034/1‑139 (MQ=255)
                                               tCCGATTGCTGTTGGCTGAACCTTTGATTTTTTGTAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGatat                                                                                                                <  3:199461/108‑1 (MQ=255)
                                               tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGatat                                                                                                                >  4:199461/1‑108 (MQ=255)
                                               tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg                                                                                                              <  6:216385/110‑1 (MQ=255)
                                               tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAg                                                                                                              >  5:216385/1‑110 (MQ=255)
                                               tCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATAAAGGTATTTAGGGTTGGGTGCGGTTATTGCAt                                                                                 >  2:393015/1‑139 (MQ=255)
                                               tCCGATTGCTGTTGGCTGAACATTGGATTTTTTGAAAAATGAAGCCATATTTTCTTTCATTTACCTTATTTGACAAGCTTTTGAAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGTTTTTGGTGCGTTTGTTGCAt                                                                                 >  2:385674/1‑139 (MQ=255)
                                                ccGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg                                                                                <  6:423828/139‑1 (MQ=255)
                                                ccGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg                                                                                >  7:70759/1‑139 (MQ=255)
                                                ccGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGGTTTTGCATg                                                                                <  8:165556/139‑1 (MQ=255)
                                                ccGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg                                                                                >  8:461968/1‑139 (MQ=255)
                                                 ctaTTGCTTTTTGCTGAACCTTGTATTTTTTGAAAAATGAAGCACTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTTCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg                                                                                <  1:385674/136‑1 (MQ=255)
                                                 cGATTGCTGTTGGCTGAACCTTGGATTTTTTGAATAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATg                                                                                <  1:393015/138‑1 (MQ=255)
                                                 cGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATgg                                                                               <  3:12572/139‑1 (MQ=255)
                                                   aTTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAATCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTATTTGGATGGCg                                                                             >  2:520754/1‑139 (MQ=255)
                                                    ttGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCgg                                                                            >  6:486042/1‑139 (MQ=255)
                                                     tGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGt                                                                           >  5:347078/1‑139 (MQ=255)
                                                     tGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACATTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGGATGGGGGt                                                                           >  5:86002/1‑139 (MQ=255)
                                                        tGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       >  4:202639/1‑92 (MQ=255)
                                                        tGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTcag                                                                                                                       <  3:202639/92‑1 (MQ=255)
                                                        tGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca                                                                        >  1:121110/1‑139 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAATCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTATGGTTGGGGGCGGTTTTTGCATGGCGGTTca                                                                        <  5:87284/137‑1 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcaca                                                                      <  2:87293/139‑1 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcaca                                                                      <  7:527034/139‑1 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                       <  5:446445/138‑1 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                       <  2:121110/138‑1 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                       <  2:421233/138‑1 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca                                                                        >  1:87293/1‑137 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTGGGGGGCGGGTTTTGCATGGCGGTTcac                                                                       <  8:333635/138‑1 (MQ=255)
                                                          ttGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTACTTTGACAAGCTTTTGTTAAGCCCGTATTATCAGCAGATATAGGTATTTAGGGTTGGCGGCGGTTTTTTCATGGCGGTTca                                                                        >  5:320183/1‑137 (MQ=255)
                                                          taggCTGAACCTTGGATATTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTca                                                                        <  2:180198/135‑1 (MQ=255)
                                                                 aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGTTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                               <  2:402581/91‑1 (MQ=255)
                                                                 aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGAtata                                                                                                               >  1:402581/1‑91 (MQ=255)
                                                                 aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTaaa                                                               >  6:226879/1‑139 (MQ=255)
                                                                 aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTaaa                                                               >  6:501469/1‑139 (MQ=255)
                                                                 aaCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTaaa                                                               >  3:446860/1‑139 (MQ=255)
                                                                  aCCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTaaa                                                               <  6:333947/138‑1 (MQ=255)
                                                                      tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGc                                                                                            <  5:421594/105‑1 (MQ=255)
                                                                      tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGc                                                                                            >  6:421594/1‑105 (MQ=255)
                                                                      tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTcac                                                                       <  5:292556/126‑1 (MQ=255)
                                                                      tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCt                                                          >  8:98798/1‑139 (MQ=255)
                                                                      tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCt                                                          >  2:198557/1‑139 (MQ=255)
                                                                      tGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGGTcac                                                                       >  6:292556/1‑126 (MQ=255)
                                                                             tttGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCTGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTACACTGCTTTTGa                                                     >  4:46370/1‑137 (MQ=255)
                                                                             tttGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGaaa                                                   >  1:20901/1‑139 (MQ=255)
                                                                             tttGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGaa                                                    <  7:461968/138‑1 (MQ=255)
                                                                                gAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGa                                                 <  2:20901/138‑1 (MQ=255)
                                                                                 aaaaaTGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGAtt                                               <  5:226879/139‑1 (MQ=255)
                                                                                                   tctttcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCa                              <  4:456509/138‑1 (MQ=255)
                                                                                                   tcttacCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCa                              <  7:258431/138‑1 (MQ=255)
                                                                                                      ttcCTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGTGTTGGGGGCGGTTTTGGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGTTGGGCTGCa                              >  5:73293/1‑135 (MQ=255)
                                                                                                         cTTTACCTTCTTTGATAAGCTTTTGTAAAGCCCGGTTTTTCAGCAGATATAGGTATTTAGGGGTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt                        <  6:320183/138‑1 (MQ=255)
                                                                                                         cTTTACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATTTAGGTATTTAGGGTTGGGGGCGGATTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCtt                        <  1:74874/138‑1 (MQ=255)
                                                                                                          tttACCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTTGTTTTTAAAGATTTTATACGGTTTGATGCAGATCTTTc                      >  5:317638/1‑139 (MQ=255)
                                                                                                             aCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGGTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                    <  5:501469/138‑1 (MQ=255)
                                                                                                             aCCTTCTTTGACAAGCTTTTGTAAAGCCCGTTTTTCCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGt                    <  8:539254/138‑1 (MQ=255)
                                                                                                                         aaGCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtcg       >  1:526786/1‑139 (MQ=255)
                                                                                                                           gCTTTTGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGgtcgtc     >  2:293752/1‑139 (MQ=255)
                                                                                                                             ttttGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTCTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTGCa   >  4:267458/1‑139 (MQ=255)
                                                                                                                               ttGTAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCat  <  2:526786/138‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                      
TAGTTGGAGGCATGTGAAAATAGTAGTCTCCGATTGAGATAAGCACGTCCGATTGCTGTTGGCTGAACCTTGGATTTTTTGAAAAATGAAGCCCTATTTTCTTTCCTTTACCTTCTTTGACAAGCTTTTGCAAAGCCCGTTTTTTCAGCAGATATAGGTATTTAGGGTTGGGGGCGGTTTTTGCATGGCGGTTCACAGTGTAAACTGCTTTTGAAAGATTTTCTACGGTTGGCTGCAGATCTTTCGTATATGCATGGTCGTCCAT  >  BS168_reference_genome/1474760‑1475024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A