Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F66 I1 R1	33	61.7	2789148	95.4%	2660847	127.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,271,424	T→C	R78R (AGA→AGG)	uvrX ←	lesion bypass phage DNA polymerase; phage SPbeta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,271,424	0	T	C	100.0%	200.6 / NA	70	R78R (AGA→AGG)	uvrX	lesion bypass phage DNA polymerase; phage SPbeta
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/46); total (24/46)

TTTCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAA  >  BS168_reference_genome/2271290‑2271558

tttCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCtt                                                                                                                                     <  4:259272/138‑1 (MQ=255)

 ttCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCtt                                                                                                                                     <  4:112177/137‑1 (MQ=255)

  tCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTgg                                                                                                                                   <  4:291768/138‑1 (MQ=255)

  tCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGa                                                                                                                                  >  1:204589/1‑139 (MQ=255)

    cTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATc                                                                                                                                <  1:24634/139‑1 (MQ=255)

    cTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATc                                                                                                                                <  6:127458/139‑1 (MQ=255)

      gCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCt                                                                                                                               >  1:253634/1‑138 (MQ=255)

        aTCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCtt                                                                                                                              >  4:164449/1‑137 (MQ=255)

             aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAg                                                                                                                           <  5:281382/135‑1 (MQ=255)

             aaaaaGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTAt                                                                                                                       >  8:55544/1‑139 (MQ=255)

                           aTTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCg                                                                                                         <  4:30049/139‑1 (MQ=255)

                                     tATGGACTCATTTCTCAGGAACAAATCTGTATAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGtttt                                                                                               <  4:177744/139‑1 (MQ=255)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATTTGTATCCTTGGATCTTCAg                                                                                                                           <  1:24211/102‑1 (MQ=255)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCaaa                                                                                                                 >  6:119113/1‑112 (MQ=255)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCaaa                                                                                                                 <  5:119113/112‑1 (MQ=255)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  6:114203/1‑138 (MQ=37)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  2:184841/1‑138 (MQ=37)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  6:202564/1‑138 (MQ=37)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCaa                                                                                       >  1:204463/1‑138 (MQ=37)

                                              aTTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATATGTATCCTTGGATCTTCAg                                                                                                                           >  2:24211/1‑102 (MQ=255)

                                                    cAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCttt                                                                                >  4:11794/1‑139 (MQ=21)

                                                       gAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             <  5:202564/139‑1 (MQ=21)

                                                           aaaTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGt                                                                         <  4:14368/139‑1 (MQ=21)

                                                            aaTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGATAAGCTTCATTTGTGGATTTACAATGTGTCTCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTg                                                                        <  8:118727/139‑1 (MQ=255)

                                                             aTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTg                                                                        >  8:334847/1‑138 (MQ=21)

                                                             aTCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGc                                                                       <  2:204463/139‑1 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                     <  3:235886/136‑1 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                     >  4:5959/1‑136 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                     >  6:286497/1‑136 (MQ=21)

                                                                  tAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAg                                                                     >  8:114400/1‑136 (MQ=21)

                                                                    aaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                <  8:10759/139‑1 (MQ=21)

                                                                    aaaCAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                <  8:211410/139‑1 (MQ=21)

                                                                       cAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                >  4:59996/1‑136 (MQ=21)

                                                                       cAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                <  3:59996/136‑1 (MQ=21)

                                                                         gCTTTTTAATTTCAGTTTAAACTCTTATTAAAAGCTTCATTTGTTGATTTAAACTTTGTATCCTTGGATCTTCAGGTATCTCAAATAGTATCGATCCTGTTTTTATTCCAAAATCTTTTTTAAGGGCAGGAGATGCAGc                                                           <  5:269652/139‑1 (MQ=11)

                                                                         gCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAtgctgc                                                           <  5:269650/139‑1 (MQ=21)

                                                                         gCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           <  3:153752/139‑1 (MQ=21)

                                                                         gCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           <  7:4895/139‑1 (MQ=21)

                                                                          cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           <  8:181100/138‑1 (MQ=21)

                                                                          cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           >  1:79142/1‑138 (MQ=21)

                                                                          cTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGc                                                           >  7:181100/1‑138 (MQ=21)

                                                                               tAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTaata                                                      <  1:184841/138‑3 (MQ=21)

                                                                                 aTTTCAGTTGAAACTCTTATGAAAAGATTCATTTGTGGATTTACATTGTGTATCCTTGGATCTTCAGTTATCTAAAATAGTCTCGATCCTGTTTTGATTCCAAATTATTTTTTAAGTGCAGTATATGCAGCTAatact                                                    <  7:327370/138‑1 (MQ=11)

                                                                                 aTTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAatacta                                                   <  6:264413/139‑1 (MQ=21)

                                                                                        ttGAAACTATGATGAAAAGCTTCATTTGTGTATTTACAATGTGTATCCTTGTATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATATTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTcc                                            <  7:183069/139‑1 (MQ=11)

                                                                                              ctctGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCt                                      <  7:55544/139‑1 (MQ=17)

                                                                                              ctctGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCt                                      <  4:154264/139‑1 (MQ=17)

                                                                                              ctatgatgaAAAGATTCATTTGTGTATTTACAATGTGTATCCTTGTATCTTCAGGTATCTAAAATAGTCTCGATCCTGTTTTGATTCAAAATTCTTTTTTATGTGCAGGTTATGCAGCTAATACTACACTTCCCTGTCt                                      <  2:253634/136‑1 (MQ=11)

                                                                                                 tgatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCtc                                     <  2:204589/137‑2 (MQ=12)

                                                                                                   atgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTcc                                  <  8:198860/138‑1 (MQ=12)

                                                                                                      aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                               <  8:119038/138‑1 (MQ=12)

                                                                                                      aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                               <  7:119720/138‑1 (MQ=12)

                                                                                                      aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                               <  3:116423/138‑1 (MQ=12)

                                                                                                      aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                               <  8:201516/138‑1 (MQ=12)

                                                                                                                   gtgtagttACACTGTGTATCCTTGGATCTTCAGGTATCTCAAATATTATCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCATACAACAg                  <  3:345005/132‑1 (MQ=11)

                                                                                                                          aaCAATGGGAATCCTTGGATCTTCCGGTATCTCAAATAGTCTCGCACCTGTTTTGATTCCAAAATCTTTTTTAAGGGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATa           <  2:138930/137‑1 (MQ=11)

                                                                                                                           aCAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             >  5:142835/1‑71 (MQ=21)

                                                                                                                           aCAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCtttttt                                                                             <  6:142835/71‑1 (MQ=21)

                                                                                                                           aCAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAg          >  1:11754/1‑138 (MQ=255)

                                                                                                                            cAATGTGTATCCTTGGATCTTCAGGTATCTCAATTAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc         <  6:159696/138‑1 (MQ=255)

                                                                                                                            cAATGTGGATCCTTGTAGCTTCAGGTATCTAAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAATGGCTGGAGATGCAGCTAATACTCCACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc         <  2:146106/138‑1 (MQ=11)

                                                                                                                              aTGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                <  7:58675/81‑1 (MQ=18)

                                                                                                                              aTGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAt                                                                >  8:58675/1‑81 (MQ=18)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGtt    >  4:244842/1‑138 (MQ=255)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGtt    >  8:32420/1‑138 (MQ=255)

                                                                                                                                 tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa   <  5:124160/139‑1 (MQ=255)

                                                                                                                                 tgtATCCTAGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACCGCAAGATAGCATGtt    >  8:50534/1‑138 (MQ=11)

                                                                                                                                  gtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa  <  5:23809/139‑1 (MQ=255)

                                                                                                                                   tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa  <  7:114400/138‑1 (MQ=255)

                                                                                                                                   tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTaa  <  4:101345/138‑1 (MQ=255)

TTTCCTGCATCTAAAAAAGATTCATCAATTGAATACGTATGGACACATTTCTCAGGAACAAATCTGTAAAACAGCTTTGTAATTTCAGTTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAA  >  BS168_reference_genome/2271290‑2271558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A