Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F66 I1 R1	33	61.7	2789148	95.4%	2660847	127.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	2,891,153	G→T	A322E (GCG→GAG)	leuB ←	3‑isopropylmalate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	2,891,153	0	G	T	100.0%	164.6 / NA	48	A322E (GCG→GAG)	leuB	3‑isopropylmalate dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (25/23); total (25/23)

CTGTCACACATTAGAAATTGTATTTTCACTCATGATTGCTGCCTTAACTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCGCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGCGCGGAGCCATGAACAGGTTCA  >  BS168_reference_genome/2891017‑2891287

cTGTCACACATTAGAAATTGTATTTTCACTCATGATTGCTGCCTTAACTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCa                                                                                                                                      <  3:325810/139‑1 (MQ=255)

           tAGAAATTGTATTTTCACTCATGATTGCTGCCTTAACTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGc                                                                                                                           >  6:86852/1‑139 (MQ=255)

                           aCTCATGATTGCTGCCTTAACTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCa                                                                                                           <  2:44250/139‑1 (MQ=255)

                                  aTTGCTGCCTTAACTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGa                                                                                                    <  5:313036/139‑1 (MQ=255)

                                         ccTTAACTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATg                                                                                              <  4:222622/138‑1 (MQ=255)

                                             aaCTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTtct                                                                                          >  4:169338/1‑138 (MQ=255)

                                               cTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCTAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCaa                                                                                       <  6:29961/139‑1 (MQ=255)

                                                   ctctGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAg                                                                                    >  3:338647/1‑138 (MQ=255)

                                                    tctGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCGTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTCCAGCTTTCGCTTCCTCTTCAAGCCCGAAAGAGTGACTCAAAAGCa                                                                                  >  2:320455/1‑139 (MQ=255)

                                                      tGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCa                                                                                  <  2:325670/137‑1 (MQ=255)

                                                             gCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGATGCCCAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCGGAATTATGTTTTCAAAAGCATTGCCGCt                                                                          >  5:15184/1‑138 (MQ=255)

                                                             gCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCt                                                                          <  1:168511/138‑1 (MQ=255)

                                                             gCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCt                                                                          <  5:122375/138‑1 (MQ=255)

                                                             gCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGGTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCt                                                                          <  8:340064/138‑1 (MQ=255)

                                                             gCCTGAGTGCTGCTGAACTCTTCACTCAGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCt                                                                          <  5:218618/138‑1 (MQ=255)

                                                                tGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTc                                                                                                                       <  4:219100/90‑1 (MQ=255)

                                                                tGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTc                                                                                                                       >  3:219100/1‑90 (MQ=255)

                                                                tGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGAc                                                                       >  2:10903/1‑138 (MQ=255)

                                                                  aGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACaa                                                                     >  5:65719/1‑138 (MQ=255)

                                                                  aGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACaa                                                                     >  5:75770/1‑138 (MQ=255)

                                                                  aGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACaa                                                                     >  1:295019/1‑138 (MQ=255)

                                                                       tgctgAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGTAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTGAAGCCCGAAAGATGTTCTCAAAAGAATTGCCGCTGCCAATAAGGc                                                               >  1:265809/1‑139 (MQ=255)

                                                                       tgctgAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGAt                                                                                               >  1:145058/1‑107 (MQ=255)

                                                                       tgctgAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGAt                                                                                               <  2:145058/107‑1 (MQ=255)

                                                                            aaCTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGa                                                          <  4:97273/139‑1 (MQ=255)

                                                                              ctctTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAAc                                                        >  1:304592/1‑139 (MQ=255)

                                                                                 ttCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGa                                                     >  3:172716/1‑139 (MQ=255)

                                                                                 ttCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCGTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGa                                                     >  4:144591/1‑139 (MQ=255)

                                                                                   cACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGACAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGACCGAAAGATGATCTCAAAATCATTGAAGCTGACAATATGGCTGCGAACGGAta                                                   >  8:31285/1‑138 (MQ=255)

                                                                                         gTGCCATGTCTCTTGTTCTTTTTCCAGAAGCCAAGACGTTCTTTACCTCATATTCTACAGCTTTCGCTTCGTCATCAAGCCCGAAAGATGATATCATAAGCTTTGCCGCTCTAAATATGGCTGCGAACGGAatt                                                  >  2:338194/1‑134 (MQ=255)

                                                                                           gCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATAGCCGCTGACAATATGGCTGCGAACGGATTTGCCATcc                                           >  4:202503/1‑139 (MQ=255)

                                                                                                gTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCa                                                                                  <  3:26798/95‑1 (MQ=255)

                                                                                                gTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTa                                      >  2:39880/1‑139 (MQ=255)

                                                                                                gTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTATCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCa                                                                                  >  4:26798/1‑95 (MQ=255)

                                                                                                           tttttCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAatat                            >  3:330891/1‑138 (MQ=255)

                                                                                                            ttttCAAGCATCCAAGACTTTGTTTCCCTCATCTTCTACATCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATc                           <  8:266494/138‑1 (MQ=255)

                                                                                                             tttCCAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTAGCTTCCTCTTCAACCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAAAATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAg                         >  7:253281/1‑139 (MQ=255)

                                                                                                                 cAGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGgcg                      <  4:172716/138‑1 (MQ=255)

                                                                                                                  aGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGcgcg                    <  1:10903/139‑1 (MQ=255)

                                                                                                                  aGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGcgcg                    <  6:65719/139‑1 (MQ=255)

                                                                                                                  aGAAGCCAAGACTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGcgcg                    <  1:39880/139‑1 (MQ=255)

                                                                                                                  aGAAGCCAAGACTTTGGTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGcgcg                    <  1:320455/139‑1 (MQ=255)

                                                                                                                            aCTTTGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGCGCGGAGCCATGaa          <  6:75770/139‑1 (MQ=255)

                                                                                                                            aCTTTGTTTAACTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGCGCGGAGCCATGaa          <  2:295019/139‑1 (MQ=255)

                                                                                                                                tGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGcc                                               <  6:308648/98‑1 (MQ=255)

                                                                                                                                tGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGcc                                               >  5:308648/1‑98 (MQ=255)

                                                                                                                                tGTTTACCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGAAAATATGGATGCGGACGGATTTGCCCTCCCTTTACCGGCAATATCAGGCGCGGAGCCATGAACAgg      >  2:51060/1‑139 (MQ=255)

                                                                                                                                     aCCTCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGCGCGGAGCCATGAACAGGTTCa  >  1:308137/1‑138 (MQ=255)

CTGTCACACATTAGAAATTGTATTTTCACTCATGATTGCTGCCTTAACTTCCTCTGTAATGGCCTGAGTGCTGCTGAACTCTTCACTCCGTGCCAAGTCTCTTGTTCTTTTTCCAGAAGCCAAGACTTTGTTTACCGCATCTTCTACAGCTTTCGCTTCCTCTTCAAGCCCGAAAGATGTTCTCAAAAGCATTGCCGCTGACAATATGGCTGCGAACGGATTTGCCATCCCTTTACCGGCAATATCAGGCGCGGAGCCATGAACAGGTTCA  >  BS168_reference_genome/2891017‑2891287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A