Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F66 I1 R1	33	61.7	2789148	95.4%	2660847	127.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	BS168_reference_genome	3,075,913	G→A	E183K (GAA→AAA)	ytfP →	putative NAD(FAD)‑utilizing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	BS168_reference_genome	3,075,913	0	G	A	95.7%	149.5 / ‑1.2	46	E183K (GAA→AAA)	ytfP	putative NAD(FAD)‑utilizing dehydrogenase
Reads supporting (aligned to +/- strand): ref base G (1/1); new base A (23/21); total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TATGAAGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTGAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACTAAGAGATGTAGCCGTCAGTGTGTTAAATAAAAAAG  >  BS168_reference_genome/3075781‑3076048

tatGAAGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCcg                                                                                                                                   <  2:105352/139‑1 (MQ=255)

     aGATGGACAGGCAGCCGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGGGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              <  2:31724/139‑1 (MQ=255)

     aGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              >  4:116818/1‑139 (MQ=255)

     aGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              <  7:213451/139‑1 (MQ=255)

     aGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              <  8:228931/139‑1 (MQ=255)

     aGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              <  3:180151/139‑1 (MQ=255)

     aGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              <  1:299906/139‑1 (MQ=255)

     aGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              <  6:126902/139‑1 (MQ=255)

     aGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              <  3:116818/139‑1 (MQ=255)

          gACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATCGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGAGAGACGGCTATGAATGTTCTAAAGCCGCGGGccatac                                                                                                                         >  7:308881/1‑139 (MQ=255)

          gACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGccatac                                                                                                                         >  1:286074/1‑139 (MQ=255)

          gACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGccatac                                                                                                                         >  5:150967/1‑139 (MQ=255)

            cAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGccatacca                                                                                                                       <  2:286074/139‑1 (MQ=255)

            cAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGccatacca                                                                                                                       <  6:150967/139‑1 (MQ=255)

                 agcagGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              >  8:338264/1‑127 (MQ=255)

                 agcagGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGc                                                                                                                              <  7:338264/127‑1 (MQ=255)

                      ggATTGGGACGAATACTGGCGAAATGATCCCTAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCt                                                                                                             <  6:107854/139‑1 (MQ=255)

                                                  ccATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTGAAGCcg                                                                                                                                   <  3:227348/89‑1 (MQ=255)

                                                  ccATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTGAAGCcg                                                                                                                                   >  4:227348/1‑89 (MQ=255)

                                                           ggCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCg                                                                        >  8:23475/1‑139 (MQ=255)

                                                           ggCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCg                                                                        >  1:50782/1‑139 (MQ=255)

                                                                tCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCAt                                                                   >  7:333271/1‑139 (MQ=255)

                                                                tCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCAt                                                                   >  3:323791/1‑139 (MQ=255)

                                                                tCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCAt                                                                   >  3:119508/1‑139 (MQ=255)

                                                                  attattGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCAtt                                                                  <  8:333271/138‑1 (MQ=255)

                                                                            tCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGccatacc                                                                                                                        >  2:2443/1‑74 (MQ=255)

                                                                            tCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGccatacc                                                                                                                        <  1:2443/74‑1 (MQ=255)

                                                                            tCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCaaaaaa                                                        >  1:248566/1‑138 (MQ=255)

                                                                              ggcggTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCcg                                                                                                                                   <  5:78453/61‑1 (MQ=255)

                                                                              ggcggTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCcg                                                                                                                                   >  6:78453/1‑61 (MQ=255)

                                                                              ggcggTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGccatac                                                                                                                         <  4:331193/71‑1 (MQ=255)

                                                                              ggcggTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGccatac                                                                                                                         >  3:331193/1‑71 (MQ=255)

                                                                              ggcggTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAacac                                                     >  5:150237/1‑139 (MQ=255)

                                                                              ggcggTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAacac                                                     >  5:86255/1‑139 (MQ=255)

                                                                                  gTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCTCGGGCAATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCa                                                 <  5:132750/139‑1 (MQ=255)

                                                                                         cGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTa                                          <  2:50782/139‑1 (MQ=255)

                                                                                             ccTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCAc                                      >  7:94151/1‑139 (MQ=255)

                                                                                              cTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACt                                     <  7:346069/139‑1 (MQ=255)

                                                                                                    ccGTAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACTAagag                                <  8:94151/138‑1 (MQ=255)

                                                                                                       gAAGCACGGGAGACGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACTAAGAGATg                             >  6:257082/1‑138 (MQ=255)

                                                                                                                   aCGGCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACTAAGAGATGTAGCCGTCAgtgt                >  3:92095/1‑139 (MQ=255)

                                                                                                                     ggCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACt                                     <  2:248994/116‑1 (MQ=255)

                                                                                                                     ggCTATGAATGGGCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACt                                     >  1:248994/1‑116 (MQ=255)

                                                                                                                                 gCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACTAAGAGATGTAGCCGTCAGTGTGTTAAATAAAAAAg  >  7:320299/1‑139 (MQ=255)

                                                                                                                                 gCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACTAAGAGATGTAGCCGTCAGTGTGTTAAATAAAAAAg  >  1:124399/1‑139 (MQ=255)

                                                                                                                                 gCTAAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACTAAGAGATGTAGCCGTCAGTGTGTTAAATAAAAAAg  >  7:61203/1‑139 (MQ=255)

TATGAAGATGGACAGGCAGCAGGGATTGTGACGAATAATGGCGAAATGATCCATAGCCAGGCAGTCATTATTGCTGTCGGCGGTAAAAGCGTGCCTCATACCGGAAGCACGGGAGACGGCTATGAATGGGCTGAAGCCGCGGGCCATACCATAACAGAGCTGTTTCCGACAGAGGTTCCCGTCACTTCTGGCGAACCGTTCATTAAGCAAAAAACACTTCAGGGCTTATCACTAAGAGATGTAGCCGTCAGTGTGTTAAATAAAAAAG  >  BS168_reference_genome/3075781‑3076048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A