Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A24 F66 I1 R1 37 65.5 2715618 95.2% 2585268 125.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA BS168_reference_genome 2,271,505 C→T V51V (GTG→GTA)  uvrX ← lesion bypass phage DNA polymerase; phage SPbeta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*BS168_reference_genome2,271,5050CT100.0% 174.2 / NA 58V51V (GTG→GTA) uvrXlesion bypass phage DNA polymerase; phage SPbeta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/32);  total (26/32)

TTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCT  >  BS168_reference_genome/2271378‑2271629
                                                                                                                               |                                                                                                                            
ttGAAACTCTGATGAAAAGCTTAATTTGTGTATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTcc                                                                                                                   <  2:128991/139‑1 (MQ=255)
   aaaCTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTg                                                                                                                <  6:144716/139‑1 (MQ=17)
        ctGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACtt                                                                                                                     <  4:159602/129‑1 (MQ=17)
        ctGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACtt                                                                                                                     >  3:159602/1‑129 (MQ=17)
          gatgaAAAGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTcc                                                                                                         >  2:122573/1‑139 (MQ=12)
              aaaaGCTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTa                                                                                                      <  5:295535/138‑1 (MQ=12)
                   cTTCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTccc                                                                                                 >  7:50547/1‑138 (MQ=12)
                     tCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTa                                                                                               >  6:8143/1‑138 (MQ=12)
                     tCATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTa                                                                                               >  4:288757/1‑138 (MQ=12)
                      cATTTGTGGATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTac                                                                                              <  7:137871/138‑1 (MQ=12)
                              gATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGc                                                                                        >  4:111950/1‑136 (MQ=255)
                              gATTTACAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGc                                                                                        >  2:89631/1‑136 (MQ=255)
                                   aCAATGTGTATCCTTGGATC‑TCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATATTTTTTAAGTGCAGGATATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc                                                                                <  1:188831/138‑1 (MQ=11)
                                    cAATGTGTATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGc                                                                                <  8:50547/138‑1 (MQ=255)
                                    cAATGTGTATCCTTGGATCTTCAGGTATCTCAAAACGTCACGATCCTGTTTTGATTCCAAAATCTTTTTTAAGAGCAGGAGATGCACCAAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCCAGATAGc                                                                                <  4:121480/138‑1 (MQ=11)
                                         tgtATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa                                                                          <  2:72415/139‑1 (MQ=255)
                                           tATCCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACCCTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa                                                                          >  1:214873/1‑137 (MQ=255)
                                                 tGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCGCTACAACAGCAAGATAGCATGTTAAAGGGtt                                                                   >  4:101806/1‑138 (MQ=255)
                                                 tGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGtt                                                                   >  7:302270/1‑138 (MQ=255)
                                                  ggATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGtt                                                                   <  5:8143/137‑1 (MQ=255)
                                                  ggATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGtt                                                                   >  1:333477/1‑137 (MQ=255)
                                                   gagcTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAg                                                                <  4:4816/136‑1 (MQ=255)
                                                   gATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa                                                                          <  4:65310/129‑1 (MQ=25)
                                                   gATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTa                                                                          >  3:65310/1‑129 (MQ=25)
                                                   gATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAg                                                                >  1:193845/1‑139 (MQ=255)
                                                     tCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCTTAGTACCTACAACAGCAAGATAGCATGTTAAAGGGTTACGcc                                                              >  6:327887/1‑139 (MQ=11)
                                                     tCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGcc                                                              >  4:172060/1‑139 (MQ=255)
                                                        tCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCa                                                           >  8:32095/1‑139 (MQ=255)
                                                                   aaaTAGTCTCGATCGTGTTTTGTTTCCAAATTCTTTTTTAAGTGAAGGAGTTGCTGCTTATATTACACTTCCCTGTCTCTCCGTATTCCCTACATCAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGc                                                 <  2:274934/138‑1 (MQ=255)
                                                                   aaaTAGTCTCGATCCTTTTTTTATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGc                                                 <  4:225407/138‑1 (MQ=255)
                                                                   aaaTAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTAc                                                    <  1:163319/135‑1 (MQ=255)
                                                                      tAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGa                                              >  6:115803/1‑138 (MQ=255)
                                                                         tctcGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGTGTTAAGCCCCATTGTTACAGCCGATACa                                          >  8:78947/1‑139 (MQ=255)
                                                                         tctcGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATa                                            >  3:72374/1‑137 (MQ=255)
                                                                          ctcGATCCTGTTTTTATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAg                                         <  5:8707/139‑1 (MQ=255)
                                                                          ctcGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTAGTCCCTACAACAGCACGATAGCATGTTAAAGGGTTACGCCCCAGTGTTACAGCCGATa                                            >  6:256014/1‑136 (MQ=14)
                                                                           tcGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAg                                         >  7:224209/1‑138 (MQ=255)
                                                                           tcGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAg                                         >  4:336829/1‑138 (MQ=255)
                                                                             gATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCATACAACAGAAAGATAGCATGTTAAAGGGTTAAGCCCAATTGTTACAGCCGATACAGAAg                                      >  1:274934/1‑139 (MQ=14)
                                                                             gATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGAGGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGa                                        <  8:134978/137‑1 (MQ=255)
                                                                             gATCCTGTTTTGATTCCAAAATCTTTTTAAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAg                                      <  6:132932/139‑1 (MQ=255)
                                                                              aTCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGc                                     <  1:35564/139‑1 (MQ=255)
                                                                                    ttttGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGa                                        >  2:168669/1‑130 (MQ=255)
                                                                                    ttttGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGa                                        <  1:168669/130‑1 (MQ=255)
                                                                                    tattGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATaaaa                               <  5:247281/137‑1 (MQ=255)
                                                                                         aTTCCAAAATCTTTTTTAATTGAAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTACCTACAACAGCAAGATAGCATGTTAAAGTGTTAAGCCCCATTGTTACAGCCGAGACAGAAGCATAAATGGAtt                          >  8:65304/1‑139 (MQ=14)
                                                                                          ttCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGAttt                         <  7:78947/139‑1 (MQ=255)
                                                                                                      ttttAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGa                 <  3:111950/135‑1 (MQ=255)
                                                                                                       tttAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca             <  8:224209/138‑1 (MQ=255)
                                                                                                       tttAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca             <  4:292898/138‑1 (MQ=255)
                                                                                                       tttAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca             <  5:115803/138‑1 (MQ=255)
                                                                                                       tttAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca             <  1:122573/138‑1 (MQ=255)
                                                                                                       tttAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca             <  3:336829/138‑1 (MQ=255)
                                                                                                       tttAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGAcaca             <  3:172060/138‑1 (MQ=255)
                                                                                                         tAAGTGCAGGAGATGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACaaa           <  5:327887/138‑1 (MQ=255)
                                                                                                                 gtagaTGCAGCTAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCt  <  3:101806/137‑1 (MQ=255)
                                                                                                                            tAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGtt                                                                   <  7:79546/63‑1 (MQ=255)
                                                                                                                            tAATACTACACTTCCCTGTCTCTCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGtt                                                                   >  8:79546/1‑63 (MQ=255)
                                                                                                                               |                                                                                                                            
TTGAAACTCTGATGAAAAGCTTCATTTGTGGATTTACAATGTGTATTCTTGGATCTTCAGGTATCTCAAATAGTCTCGATCCTGTTTTGATTCCAAAATCTTTTTTAAGTGCAGGAGATGCAGCTAACACTACACTTCCCTGTCTATCCGTATTCCCTACAACAGCAAGATAGCATGTTAAAGGGTTAAGCCCCATTGTTACAGCCGATACAGAAGCATAAAAGGATTTCATATCGACACAAAGTATATTCT  >  BS168_reference_genome/2271378‑2271629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A