Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F68 I0 R1 412 52.0 1996146 94.7% 1890350 135.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,165,449 C→T 20.2% noncoding (50/85 nt) tyrU → tRNA‑Tyr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,165,4490CT20.2% 185.6 / 42.4 79noncoding (50/85 nt)tyrUtRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (38/25);  new base T (3/13);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 4.34e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

GATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAG  >  CP009273/4165313‑4165582
                                                                                                                                        |                                                                                                                                     
gATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGa                                                                                                                                    >  1:118804/1‑140 (MQ=255)
gATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGa                                                                                                                                    >  1:118785/1‑140 (MQ=255)
      gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGGTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                              <  2:274122/140‑1 (MQ=255)
       tcTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:459658/139‑1 (MQ=255)
       gcTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:975000/139‑1 (MQ=255)
       aCTTAGCTCAGTAGTTAGAGCAACTGACTTGTAATCAGTATGTCACCTGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGTTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:140770/140‑1 (MQ=37)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGTACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:673965/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCACGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:215319/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:62482/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:62491/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:377323/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:835505/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:926026/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:370958/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:850965/140‑1 (MQ=255)
       aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:707357/140‑1 (MQ=255)
           aGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGtt                                                                                                                         >  2:859539/1‑140 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             >  1:123432/1‑128 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             >  1:836448/1‑128 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             >  1:837754/1‑128 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:123432/128‑1 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:836448/128‑1 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:837754/128‑1 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                 >  1:518902/1‑140 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                 >  2:792187/1‑140 (MQ=255)
                   aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                 >  1:458829/1‑140 (MQ=255)
                      tagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTcc                                                                                                              <  2:424611/140‑1 (MQ=255)
                       agagCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTccc                                                                                                             >  1:800203/1‑140 (MQ=255)
                         agCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTccccc                                                                                                           <  2:118804/140‑1 (MQ=255)
                         agCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTccccc                                                                                                           <  2:118785/140‑1 (MQ=255)
                          gCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGCCCAAAGGGAGCAGACAGTCAATCTGCCGTCACAGACTTCGAAGGTTCGAATCATTACCcca                                                                                                          >  1:280755/1‑140 (MQ=255)
                              cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac                                                                                                      >  2:62966/1‑140 (MQ=255)
                               tGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTACGAAGGTTCGAATCCTTCCCccaccacc                                                                                                     <  1:701344/140‑1 (MQ=255)
                                gACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccacca                                                                                                    >  1:668487/1‑140 (MQ=255)
                                gACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccacca                                                                                                    >  1:266193/1‑140 (MQ=255)
                                        aTCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGc                                                                                            >  2:386944/1‑140 (MQ=255)
                                           aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAc                                                                                         >  2:903415/1‑140 (MQ=255)
                                           aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAc                                                                                         >  1:220585/1‑140 (MQ=255)
                                           aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAc                                                                                         >  2:234366/1‑140 (MQ=255)
                                              aGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgcg                                                                                      <  1:775523/140‑1 (MQ=255)
                                                    ccAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCg                                                                                <  2:43259/140‑1 (MQ=255)
                                                          cGATTCCGGTAGTAGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGccc                                                                          <  2:217345/140‑1 (MQ=255)
                                                          cGATTCCGGTAGTAGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGccc                                                                          <  2:638380/140‑1 (MQ=255)
                                                           gATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCg                                                                         >  1:503347/1‑140 (MQ=255)
                                                            aTTCCGGTCGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCga                                                                        <  1:128047/140‑1 (MQ=255)
                                                            aTTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCga                                                                        <  1:282916/140‑1 (MQ=255)
                                                               ccGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                              >  1:487350/1‑83 (MQ=255)
                                                               ccGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                              <  2:487350/83‑1 (MQ=255)
                                                               ccGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGAc                                                                     <  1:639493/140‑1 (MQ=255)
                                                                               tCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTAcc                                                     >  1:482995/1‑140 (MQ=255)
                                                                                    tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  1:400693/63‑1 (MQ=255)
                                                                                    tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             >  2:400693/1‑63 (MQ=255)
                                                                                    tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                >  1:215022/1‑140 (MQ=255)
                                                                                           gggTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATaaa                                         <  1:146351/140‑1 (MQ=255)
                                                                                           gggTTCCAGAGCGGCAAAAGGGATAAGACTGTAAATCTGCCGTCATCGACTTCGAATGTTCGAATCCTTCCCCCAACACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATaaa                                         <  1:567009/140‑1 (MQ=25)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                                                                                                               <  1:163808/51‑1 (MQ=255)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                                                                                                               >  2:163808/1‑51 (MQ=255)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCg                                                                                >  1:90340/1‑98 (MQ=255)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCg                                                                                <  2:90340/98‑1 (MQ=255)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                      >  2:963981/1‑140 (MQ=255)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                      >  2:896076/1‑140 (MQ=255)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                      >  2:740041/1‑140 (MQ=255)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                      >  2:879022/1‑140 (MQ=255)
                                                                                              ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                      >  2:361575/1‑140 (MQ=255)
                                                                                                    aGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtc                                <  1:328606/140‑1 (MQ=255)
                                                                                                    aGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtc                                <  1:455539/140‑1 (MQ=255)
                                                                                                      cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCCCGAATAAAGAGAGCTtctc                              >  2:803353/1‑140 (MQ=255)
                                                                                                       ggCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGa                                              >  1:826538/1‑123 (MQ=255)
                                                                                                       ggCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGa                                              <  2:826538/123‑1 (MQ=255)
                                                                                                       ggCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctct                             >  2:596157/1‑140 (MQ=255)
                                                                                                       ggCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctct                             >  2:308933/1‑140 (MQ=255)
                                                                                                        gCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctctc                            >  1:700033/1‑140 (MQ=255)
                                                                                                         ccAAAGGGAGCAGACTGTAAATCTGCGGTCACAGACTTCGTAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCg                           <  2:280755/140‑1 (MQ=255)
                                                                                                                   cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAAGTTCGGCCACGCGATGGCGTAGCCCGAGACGATACGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTg                 >  1:804841/1‑140 (MQ=255)
                                                                                                                                tGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATc    >  1:989114/1‑140 (MQ=255)
                                                                                                                                tGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATc    >  1:539121/1‑140 (MQ=255)
                                                                                                                                tGCCGTCACAGACTTCGAAGGGTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCGGTGCAGAATGAAAATc    >  1:149617/1‑140 (MQ=255)
                                                                                                                                  ccGTCACAGACTTCGACGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATCAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGCTCTGCAGTGCAGAATGAAAATCAg  >  1:984741/1‑140 (MQ=255)
                                                                                                                                  ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAg  >  1:712399/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
GATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAG  >  CP009273/4165313‑4165582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A