Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F69 I0 R1 142 113.0 4248520 96.3% 4091324 135.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,165,449 C→T 12.4% noncoding (50/85 nt) tyrU → tRNA‑Tyr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,165,4490CT12.4% 444.0 / 44.4 145noncoding (50/85 nt)tyrUtRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (69/58);  new base T (2/16);  total (71/74)
Fisher's exact test for biased strand distribution p-value = 6.68e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 146 total aligned reads displayed.

TTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAG  >  CP009273/4165311‑4165582
                                                                                                                                          |                                                                                                                                     
       cGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                                                                                                               >  2:1010726/1‑140 (MQ=255)
       cGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                                                                                                               >  2:1014038/1‑140 (MQ=255)
       cGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                                                                                                               >  1:1234331/1‑140 (MQ=255)
       cGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                                                                                                               >  1:1328105/1‑140 (MQ=255)
       cGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                                                                                                               >  2:770373/1‑140 (MQ=255)
       cGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCg                                                                                                                               >  2:406041/1‑140 (MQ=255)
        gACTTATCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                              <  2:1114480/140‑1 (MQ=255)
        gACTTAGCTGAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAACTCTGCCGTCATCGACTTCGa                                                                                                                              <  2:957710/140‑1 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                              <  2:860889/140‑1 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                              <  2:1646907/140‑1 (MQ=255)
        gACTGAGCTCAGGAGGGAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                              <  2:1685282/140‑1 (MQ=255)
         ccTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:656678/139‑1 (MQ=255)
         aCTTAGCTCAGTCGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGAGTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:2033222/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTCGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAACTCGGCCGTCATCGACTTCGaa                                                                                                                             <  2:329453/140‑1 (MQ=37)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:1996389/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:1891839/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:165024/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:1568868/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:911096/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:1814448/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:1694849/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:301780/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:1394377/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                             >  1:1192705/1‑140 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGACCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:1261709/140‑1 (MQ=255)
         aCTGAGCTCACTAGGTAGAGCAACTGACTGGTAAGCAGTAGGTCACCAGTTCGATGCCGGTAGTCGGCACCATCAAGTCCGGTGGGGGTCCCGAGCGGCCAAAGGGCGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:1826324/140‑1 (MQ=25)
             aGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGtt                                                                                                                         >  1:563422/1‑140 (MQ=255)
              gCTCATTAGGTATAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTGCGTTGGGGTTCCCGAGCGGCCAAAGGGTGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTc                                                                                                                        <  2:539115/140‑1 (MQ=37)
              gCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGCGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTc                                                                                                                        <  2:328961/140‑1 (MQ=255)
              gCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTc                                                                                                                        <  2:1734324/140‑1 (MQ=255)
              gCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTc                                                                                                                        <  2:439906/140‑1 (MQ=255)
               cTCAGGAGGTAGAGCAACTGAATTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCg                                                                                                                       <  1:1079787/140‑1 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAGTCTGCCGTCACAGACTTCGaa                                                                                                                             <  2:478134/128‑1 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAGTCTGCCGTCACAGACTTCGaa                                                                                                                             >  1:478134/1‑128 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             <  2:592919/128‑1 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                             >  1:592919/1‑128 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                 >  1:757290/1‑140 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                 >  2:1683860/1‑140 (MQ=255)
                       gtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTc                                                                                                               >  2:955285/1‑140 (MQ=255)
                            gCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCcca                                                                                                          <  2:1582655/140‑1 (MQ=255)
                            gCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCcca                                                                                                          >  2:244184/1‑140 (MQ=255)
                            gCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCcca                                                                                                          <  2:1036129/140‑1 (MQ=255)
                                cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac                                                                                                      >  1:262463/1‑140 (MQ=255)
                                cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac                                                                                                      >  1:1667547/1‑140 (MQ=255)
                                cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac                                                                                                      >  2:963726/1‑140 (MQ=255)
                                cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac                                                                                                      >  2:495865/1‑140 (MQ=255)
                                cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac                                                                                                      >  1:1212643/1‑140 (MQ=255)
                                  gACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccacca                                                                                                    >  2:1469298/1‑140 (MQ=255)
                                       gTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTc                                                                                               >  1:1772823/1‑140 (MQ=255)
                                           tCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGcc                                                                                           <  2:757290/140‑1 (MQ=255)
                                            cAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCa                                                                                          <  1:1469298/140‑1 (MQ=255)
                                             aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAc                                                                                         >  2:2078560/1‑140 (MQ=255)
                                             aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAc                                                                                         >  1:558078/1‑140 (MQ=255)
                                               tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgc                                                                                       >  2:1715187/1‑140 (MQ=255)
                                               tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgc                                                                                       >  2:674708/1‑140 (MQ=255)
                                                aGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgcg                                                                                      <  1:1519648/140‑1 (MQ=255)
                                                aGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgcg                                                                                      <  2:78826/140‑1 (MQ=255)
                                                aGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgcg                                                                                      <  2:467948/140‑1 (MQ=255)
                                                     aCCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGc                                                                                 <  1:413182/140‑1 (MQ=255)
                                                     aCCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGc                                                                                 <  1:996211/140‑1 (MQ=255)
                                                      ccAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCg                                                                                <  1:955285/140‑1 (MQ=255)
                                                         gTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGCCTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAg                                                                             >  1:2124013/1‑140 (MQ=255)
                                                         gTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAg                                                                             >  1:256519/1‑140 (MQ=255)
                                                            cGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGccc                                                                          <  2:127632/140‑1 (MQ=255)
                                                              aTTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGTGATGGCGTAGCCCga                                                                        <  2:1047624/140‑1 (MQ=255)
                                                                 ccGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGAc                                                                     <  1:1662247/140‑1 (MQ=255)
                                                                         cGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCg                                                                                >  2:952809/1‑121 (MQ=255)
                                                                                 tCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTAcc                                                     >  1:293627/1‑140 (MQ=255)
                                                                                   aaGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCgg                                                   <  1:1683860/140‑1 (MQ=255)
                                                                                   aaGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCgg                                                   <  2:565274/140‑1 (MQ=255)
                                                                                     gTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCt                                                 <  1:2109833/140‑1 (MQ=255)
                                                                                      tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                >  1:706170/1‑140 (MQ=255)
                                                                                         ggTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGaa                                             >  2:368726/1‑140 (MQ=255)
                                                                                         ggTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGaa                                             >  1:789159/1‑140 (MQ=255)
                                                                                             gggTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATaaa                                         <  2:659737/140‑1 (MQ=255)
                                                                                               gTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAaga                                       >  1:1633698/1‑140 (MQ=255)
                                                                                                  cccGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagagag                                    >  1:1388678/1‑140 (MQ=255)
                                                                                                  cccGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagagag                                    >  1:1952590/1‑140 (MQ=255)
                                                                                                      aGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtc                                <  2:558078/140‑1 (MQ=255)
                                                                                                      aGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtc                                <  2:216157/140‑1 (MQ=255)
                                                                                                      aGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtc                                <  1:1808305/140‑1 (MQ=255)
                                                                                                        cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGACTAAAGAGAGCTtctc                              >  1:1750666/1‑140 (MQ=255)
                                                                                                        cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctc                              >  1:347683/1‑140 (MQ=255)
                                                                                                        cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTgctc                              >  1:756929/1‑140 (MQ=255)
                                                                                                          gCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctctc                            >  2:1357081/1‑140 (MQ=255)
                                                                                                          gCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctctc                            >  2:1250067/1‑140 (MQ=255)
                                                                                                           ccAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCg                           <  2:1274444/140‑1 (MQ=255)
                                                                                                           ccAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCg                           <  1:473671/140‑1 (MQ=255)
                                                                                                           ccAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCg                           <  1:244184/140‑1 (MQ=255)
                                                                                                           ccAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAAGCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCg                           <  2:1426388/140‑1 (MQ=255)
                                                                                                                 ggAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGATCGAATAAAGAGAGCTTCTCTCGATATTc                     <  1:797582/140‑1 (MQ=255)
                                                                                                                     cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTg                 >  1:1751579/1‑140 (MQ=255)
                                                                                                                     cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTg                 >  1:240595/1‑140 (MQ=255)
                                                                                                                          tGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGaa            >  1:807200/1‑140 (MQ=255)
                                                                                                                          tGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGaa            >  1:910732/1‑140 (MQ=255)
                                                                                                                           gTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATCTTCAGTGCAGAAt           >  1:1226858/1‑140 (MQ=255)
                                                                                                                           gTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAAt           >  1:1787574/1‑140 (MQ=255)
                                                                                                                           gTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAAt           >  2:960129/1‑140 (MQ=255)
                                                                                                                                    ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAg  >  1:870000/1‑140 (MQ=255)
                                                                                                                                    ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAg  >  1:1446451/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                     
TTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAG  >  CP009273/4165311‑4165582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A