Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F67 I0 R1 279 64.3 2479458 95.9% 2377800 135.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,165,450 A→C 16.1% noncoding (51/85 nt) tyrU → tRNA‑Tyr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,165,4500AC16.1% 223.0 / 30.2 87noncoding (51/85 nt)tyrUtRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base A (39/34);  new base C (3/11);  total (42/45)
Fisher's exact test for biased strand distribution p-value = 4.02e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

TTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCC  >  CP009273/4165311‑4165588
                                                                                                                                           |                                                                                                                                            
ttGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTcaca                                                                                                                                              <  1:956337/140‑1 (MQ=255)
ttGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTcaca                                                                                                                                              <  1:595668/140‑1 (MQ=255)
  gATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGa                                                                                                                                            <  2:727433/140‑1 (MQ=255)
  gATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGa                                                                                                                                            <  2:826484/140‑1 (MQ=255)
  gATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGCCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGa                                                                                                                                            <  2:135650/140‑1 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                                      <  2:27516/140‑1 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                                      <  2:1000297/140‑1 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                                      <  2:106446/140‑1 (MQ=255)
        gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGAGTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                                      <  2:1183555/140‑1 (MQ=255)
         aCTTAGCTCAGTAGTTAGAGCAACTGACTTGTAATAAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATGAAGGCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTAGaa                                                                                                                                     <  2:382894/140‑1 (MQ=37)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     <  2:169184/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     <  2:672534/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     <  2:565690/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     <  2:447264/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:81914/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:294029/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:299093/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:32872/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:446827/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:478108/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:496183/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:171115/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:1052264/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:987282/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:562943/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:1036732/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:878178/140‑1 (MQ=255)
         aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGAATGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     <  2:1202809/140‑1 (MQ=255)
                    taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACATACTTCGAAGGTTCGAATcc                                                                                                                          >  2:1181506/1‑140 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     <  2:1159548/128‑1 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     >  1:1159548/1‑128 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                         >  1:1092672/1‑140 (MQ=255)
                     aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                         >  1:61750/1‑140 (MQ=255)
                           agCAACTGACTTGTAATCAGTAGTTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTccccc                                                                                                                   <  2:999004/140‑1 (MQ=255)
                           agCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGGTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTccccc                                                                                                                   <  2:940013/140‑1 (MQ=255)
                              aaCTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     <  2:292890/119‑1 (MQ=255)
                              aaCTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                     >  1:292890/1‑119 (MQ=255)
                              aaCTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:379027/119‑1 (MQ=255)
                              aaCTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     >  1:379027/1‑119 (MQ=255)
                                  gACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccacca                                                                                                            >  2:182993/1‑140 (MQ=255)
                                        tAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCg                                                                                                     >  2:50587/1‑140 (MQ=255)
                                        tAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCg                                                                                                     >  2:1201708/1‑140 (MQ=255)
                                           tCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGcc                                                                                                  <  1:1095814/140‑1 (MQ=255)
                                             aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAc                                                                                                >  2:758479/1‑140 (MQ=255)
                                             aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAc                                                                                                >  1:806987/1‑140 (MQ=255)
                                               tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAc‑gc                                                                                             >  1:49063/1‑140 (MQ=255)
                                                aGGTCACCAGTTCGAGTCCGGTAGGCGGCACCATCACGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGCTTCGAATCCTTCCCCCACCACCA‑CTTTCGGCCAc‑gcg                                                                                            <  2:997606/140‑1 (MQ=21)
                                                  gTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     >  1:299509/1‑99 (MQ=255)
                                                  gTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     >  1:299574/1‑99 (MQ=255)
                                                  gTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:299574/99‑1 (MQ=255)
                                                  gTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                     <  2:299509/99‑1 (MQ=255)
                                                       cAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGt                                                                                     >  1:843282/1‑140 (MQ=255)
                                                                          ggCACCATCAAGTCCGGTGGGGTTCCCGGGCGGCCCAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTGCGAAGGTTCGAATCCTTCCGCCACCCCCA‑GTTTCGGCCCG‑GCGATGGGGGAGCCCGAGACGCTACGttg                                                                  >  2:399561/1‑139 (MQ=18)
                                                                               cATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTACATCTGCCGTCACAGACTTCGAAGGTGCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTa                                                             >  1:62829/1‑140 (MQ=255)
                                                                               cATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATCAGTTCGCTTa                                                             >  1:812790/1‑140 (MQ=255)
                                                                                 tCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTAcc                                                           >  2:59712/1‑140 (MQ=255)
                                                                                 tCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTAcc                                                           >  2:580127/1‑140 (MQ=255)
                                                                                     gTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCt                                                       <  2:49063/140‑1 (MQ=255)
                                                                                         ggTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGaa                                                   >  2:1191245/1‑140 (MQ=255)
                                                                                         ggTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGaa                                                   >  2:1193332/1‑140 (MQ=255)
                                                                                          gTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAAt                                                  >  2:270886/1‑140 (MQ=255)
                                                                                           tGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑TCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATa                                                 >  1:892908/1‑140 (MQ=255)
                                                                                           tGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCg                                                                                      >  2:72107/1‑103 (MQ=255)
                                                                                           tGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCg                                                                                      <  1:72107/103‑1 (MQ=255)
                                                                                           tGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATa                                                 <  1:491020/140‑1 (MQ=255)
                                                                                           tGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGACGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑TCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATa                                                 >  1:21053/1‑140 (MQ=255)
                                                                                             gggTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATaaa                                               <  1:50587/140‑1 (MQ=255)
                                                                                             gggTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATaaa                                               <  1:1201708/140‑1 (MQ=255)
                                                                                             gggTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATaaa                                               <  2:816749/140‑1 (MQ=255)
                                                                                                ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                            >  2:1066797/1‑140 (MQ=255)
                                                                                                  cccGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagagag                                          >  1:875034/1‑140 (MQ=255)
                                                                                                        cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATAATTC‑ACCACAGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtct                                     >  1:789973/1‑140 (MQ=17)
                                                                                                        cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctc                                    >  1:798690/1‑140 (MQ=255)
                                                                                                         ggCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctct                                   >  2:1235925/1‑140 (MQ=255)
                                                                                                         ggCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctct                                   >  2:162162/1‑140 (MQ=255)
                                                                                                              aaGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATAATTC‑ACCACAGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGat                               <  2:789973/140‑1 (MQ=17)
                                                                                                                     cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTg                       >  2:204716/1‑140 (MQ=255)
                                                                                                                     cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTg                       >  1:851090/1‑140 (MQ=255)
                                                                                                                     cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTg                       >  1:161837/1‑140 (MQ=255)
                                                                                                                     cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTg                       >  2:1103172/1‑140 (MQ=255)
                                                                                                                     cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTg                       >  2:1080384/1‑140 (MQ=255)
                                                                                                                       gACTGTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCa                     <  2:457628/140‑1 (MQ=255)
                                                                                                                          tGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAAt                                                  >  2:429448/1‑108 (MQ=255)
                                                                                                                          tGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAAt                                                  <  1:429448/108‑1 (MQ=255)
                                                                                                                                    ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAc‑gcg                                                                                            >  2:1099359/1‑56 (MQ=255)
                                                                                                                                    ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAc‑gcg                                                                                            <  1:1099359/56‑1 (MQ=255)
                                                                                                                                      gTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGt      >  2:240295/1‑140 (MQ=255)
                                                                                                                                      gTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGt      >  2:490704/1‑140 (MQ=255)
                                                                                                                                      gTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGt      >  2:327185/1‑140 (MQ=255)
                                                                                                                                          caGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGcc  <  2:1174867/140‑1 (MQ=255)
                                                                                                                                          caGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGcc  <  2:1166140/140‑1 (MQ=255)
                                                                                                                                          caGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATGTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGcc  <  2:1235727/140‑1 (MQ=255)
                                                                                                                                          caGACTTCGAAGGTTCGAATCCGTCCCCCACCACCA‑ATTTCGGCCAC‑GCGAGGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGcc  <  2:434601/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TTGATGCCGACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCA‑ATTTCGGCCAC‑GCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAGCC  >  CP009273/4165311‑4165588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A