Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F61 I0 R1 274 47.1 1889746 95.1% 1797148 135.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,165,449 C→T 27.2% noncoding (50/85 nt) tyrU → tRNA‑Tyr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,165,4490CT27.2% 164.1 / 73.2 92noncoding (50/85 nt)tyrUtRNA‑Tyr
Reads supporting (aligned to +/- strand):  ref base C (32/35);  new base T (7/18);  total (39/53)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAG  >  CP009273/4165319‑4165586
                                                                                                                                  |                                                                                                                                         
gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                                  <  2:170649/140‑1 (MQ=255)
gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                                  <  1:417422/140‑1 (MQ=255)
gACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGa                                                                                                                                  <  2:925333/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:113308/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:812102/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:445210/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:855797/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:288453/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:265924/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:593209/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:161121/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:590899/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:146623/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:821674/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:40390/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:173057/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:78706/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:821656/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:806417/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:488612/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGGAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTACATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:427306/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGAGTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:638841/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGAGTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:455997/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCACTATGTCACGAGTTCTATTCCGGTAGTCGGCTCCATCAAGTCCGGTGGGGGTGCCGAGCGGGAAAAGGGAGAAGACGGTAAATCTGCCGTAACAGACTTCGaa                                                                                                                                 <  2:259012/140‑1 (MQ=255)
 aCTTAGCTCAGTAGGTAGAGCAACGGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:517748/140‑1 (MQ=255)
 aCTGAGCTCAGTAGGGAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGAACCATCAAGTCCGGTGTGTTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:547111/140‑1 (MQ=37)
            taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                                  <  2:218393/128‑1 (MQ=255)
            taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                                  >  1:722336/1‑128 (MQ=255)
            taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                                  >  1:218393/1‑128 (MQ=255)
            taggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGa                                                                                                                                  <  2:722336/128‑1 (MQ=255)
             aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 >  1:552967/1‑128 (MQ=255)
             aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:552967/128‑1 (MQ=255)
             aggtagAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCt                                                                                                                     >  1:145300/1‑140 (MQ=255)
                      aaCTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 >  1:604222/1‑119 (MQ=255)
                      aaCTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:604222/119‑1 (MQ=255)
                        cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGCGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac                                                                                                          >  2:100370/1‑140 (MQ=255)
                        cTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCccaccac                                                                                                          >  2:129245/1‑140 (MQ=255)
                                tAATCAGGAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCg                                                                                                  >  1:766251/1‑140 (MQ=255)
                                   tCAGTTGGTCTCCAGGTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGcc                                                                                               <  2:225606/140‑1 (MQ=255)
                                     aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 >  1:162321/1‑104 (MQ=255)
                                     aGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:162321/104‑1 (MQ=255)
                                      gTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcg                                                                                            >  1:611177/1‑140 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:435589/102‑1 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 >  1:650911/1‑102 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 >  1:435589/1‑102 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:650911/102‑1 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:365151/102‑1 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 >  1:372731/1‑102 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 <  2:372731/102‑1 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCATCGACTTCGaa                                                                                                                                 >  1:365151/1‑102 (MQ=255)
                                       tAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCAcgc                                                                                           >  2:307914/1‑140 (MQ=255)
                                              ccAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCg                                                                                    <  1:629877/140‑1 (MQ=255)
                                                         ccGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGAc                                                                         <  1:76726/140‑1 (MQ=255)
                                                         ccGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGGAGCCCGAGAc                                                                         <  1:143886/140‑1 (MQ=255)
                                                                    cACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:7199/73‑1 (MQ=255)
                                                                    cACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 >  1:7199/1‑73 (MQ=255)
                                                                       cATCAAGTCCGGTGGGTTTCCAGAGCGGCCCAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCAACACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTa                                                           <  1:181049/140‑1 (MQ=255)
                                                                       cATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTa                                                           <  1:861019/140‑1 (MQ=255)
                                                                             gTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCt                                                     <  1:129245/140‑1 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCga                                                                            >  2:898369/1‑116 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCga                                                                            <  1:898369/116‑1 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                    >  1:454878/1‑140 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                    >  1:223003/1‑140 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                    >  1:273788/1‑140 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                    >  1:355798/1‑140 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                    >  1:387249/1‑140 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                    >  1:854944/1‑140 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTc                                                    >  1:914240/1‑140 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGCCCACGCGATGGCGTAGCCCGAGACGATAAGTGCGCTTACCGGCTc                                                    >  2:838745/1‑140 (MQ=255)
                                                                              tCCGGTGGGGTTCCCGAGCGCCCCCAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGACGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGAGCAGTGCGCTTACCGGCTc                                                    >  2:52809/1‑140 (MQ=21)
                                                                                cGGTGGGTTTCCCGAGCGGACAAAGGGAGCAGACTGTACATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGa                                                  <  2:778617/140‑1 (MQ=255)
                                                                                cGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGa                                                  <  2:659413/140‑1 (MQ=255)
                                                                                cGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGa                                                  <  2:167221/140‑1 (MQ=255)
                                                                                cGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGa                                                  <  2:121906/140‑1 (MQ=255)
                                                                                        ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 >  1:761664/1‑53 (MQ=255)
                                                                                        ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGaa                                                                                                                                 <  2:761664/53‑1 (MQ=255)
                                                                                        ttCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAagag                                          >  2:690673/1‑140 (MQ=255)
                                                                                                cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctc                                  >  1:175396/1‑140 (MQ=255)
                                                                                                cGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctc                                  >  1:887111/1‑140 (MQ=255)
                                                                                                 ggCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTtctct                                 >  2:798968/1‑140 (MQ=255)
                                                                                                             cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGa                                                                                         >  1:843762/1‑72 (MQ=255)
                                                                                                             cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGa                                                                                         <  2:843762/72‑1 (MQ=255)
                                                                                                             cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTCCCGGCTCGAATAAAGAGAGCGTCTCTCGATATTCAGTg                     >  1:431982/1‑140 (MQ=255)
                                                                                                             cAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATCTTCAGTg                     >  1:334565/1‑140 (MQ=255)
                                                                                                                 cTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGa                 <  2:175396/140‑1 (MQ=255)
                                                                                                                 cTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGa                 <  2:887111/140‑1 (MQ=255)
                                                                                                                   gTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAAt               >  1:213479/1‑140 (MQ=255)
                                                                                                                    tAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATg              >  2:573632/1‑140 (MQ=255)
                                                                                                                      aaTCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGaa            <  1:942716/140‑1 (MQ=255)
                                                                                                                         cTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCGTCTCTCGATATTCAGTGCAGAATGAAAAt         >  1:166607/1‑140 (MQ=255)
                                                                                                                          tGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATc        >  2:925555/1‑140 (MQ=255)
                                                                                                                            ccGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAg      >  1:537175/1‑140 (MQ=255)
                                                                                                                                cacaGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAg  <  1:307914/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                         
GACTTAGCTCAGTAGGTAGAGCAACTGACTTGTAATCAGTAGGTCACCAGTTCGATTCCGGTAGTCGGCACCATCAAGTCCGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTGTAAATCTGCCGTCACAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCAATTTCGGCCACGCGATGGCGTAGCCCGAGACGATAAGTTCGCTTACCGGCTCGAATAAAGAGAGCTTCTCTCGATATTCAGTGCAGAATGAAAATCAGGTAG  >  CP009273/4165319‑4165586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A