Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F65 I1 R1	6	137.1	4794628	98.8%	4737092	135.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,178,501	G→T	R1075L (CGT→CTT)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,178,501	0	G	T	99.2%	483.5 / NA	131	R1075L (CGT→CTT)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (40/90); total (40/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACA  >  CP009273/4178369‑4178635

  aGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACt                                                                                                                               >  1:1871651/1‑140 (MQ=255)

        tttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAAt                                                                                                                         >  1:1440760/1‑140 (MQ=255)

        tttGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAAt                                                                                                                         <  2:1111014/140‑1 (MQ=255)

          tGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCg                                                                                                                       >  1:857172/1‑140 (MQ=255)

                 tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGc                                                                                                                <  2:1006570/140‑1 (MQ=255)

                 tttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGc                                                                                                                >  1:480558/1‑140 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:878105/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  1:1820983/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:1852938/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:2344027/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  1:1566593/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:457730/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:693490/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:153410/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  1:134811/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:1053544/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  1:115754/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  1:115742/140‑1 (MQ=255)

                  ttACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTGGATGCt                                                                                                               <  1:934069/140‑1 (MQ=255)

                  ttACTGACATGAGCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGCCCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  1:2081847/140‑1 (MQ=255)

                  ttACTGACAGGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  1:1545222/140‑1 (MQ=255)

                   tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTc                                                                                                              <  2:218561/140‑1 (MQ=255)

                   tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTc                                                                                                              >  2:2065781/1‑140 (MQ=255)

                   tACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTc                                                                                                              >  2:607954/1‑140 (MQ=255)

                     cTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAg                                                                                                            <  1:1502926/140‑1 (MQ=255)

                       gACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAggg                                                                                                          <  1:233732/140‑1 (MQ=255)

                           tGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAac                                                                                                      >  2:1755502/1‑140 (MQ=255)

                            gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                     <  2:480558/140‑1 (MQ=255)

                            gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                     <  2:1422169/140‑1 (MQ=255)

                            gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                     <  2:589794/140‑1 (MQ=255)

                            gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGGTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                     <  1:1016657/140‑1 (MQ=255)

                            gATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCGGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                     <  2:1328221/140‑1 (MQ=255)

                            gATCGACGGACAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacg                                                                                                     <  2:813966/140‑1 (MQ=255)

                             aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTACTGATCTGCTTCCGGCACTGAAAATCGTTGTTGCTCAGGGTCacga                                                                                                    >  2:1421382/1‑140 (MQ=255)

                             aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                    <  2:1953528/140‑1 (MQ=255)

                             aTCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacga                                                                                                    >  1:1251496/1‑140 (MQ=255)

                                  cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAAt                                                                                                                         >  1:1266858/1‑114 (MQ=255)

                                  cGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTc                                                                                               >  1:1062753/1‑140 (MQ=255)

                                         aCCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATccc                                                                                        <  2:440707/140‑1 (MQ=255)

                                         aCCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATccc                                                                                        <  1:1510221/140‑1 (MQ=255)

                                            aTTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAgg                                                                                     <  2:1871651/140‑1 (MQ=255)

                                             ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                    <  1:539602/140‑1 (MQ=255)

                                             ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                    <  2:210498/140‑1 (MQ=255)

                                             ttACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGt                                                                                    <  2:1194233/140‑1 (MQ=255)

                                              tACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTa                                                                                   >  1:1063220/1‑140 (MQ=255)

                                              tACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTa                                                                                   >  1:1892015/1‑140 (MQ=255)

                                                  cgTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGa                                                                               <  2:795800/140‑1 (MQ=255)

                                                  cctCAGACCGCCGAACTGACCGGTCTGTCTTCGGTGGGGGTTCTGGATTCCGTAGAACGTACCGCACTTGGTAAAGAGCTGCGTCCGGCACTGAAAATCGGTGATGCTCTGGGTAACGACGTTCTGATCCCATGTACCGa                                                                               <  2:1857709/138‑1 (MQ=255)

                                                       gACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGc                                                                          <  2:2311808/140‑1 (MQ=255)

                                                        aCCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGcc                                                                         <  2:1499626/140‑1 (MQ=255)

                                                          cgacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAg                                                                       <  1:1755502/140‑1 (MQ=255)

                                                          cgacgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAg                                                                       <  2:1513911/140‑1 (MQ=255)

                                                            acgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                                                                     >  2:1297291/1‑140 (MQ=255)

                                                            acgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcg                                                                     <  2:698333/140‑1 (MQ=255)

                                                             cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAATATCTGCTTCAGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAgcgc                                                                    >  2:2286781/1‑140 (MQ=255)

                                                             cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:366813/97‑1 (MQ=255)

                                                             cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               >  1:366813/1‑97 (MQ=255)

                                                             cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgac                                                                                                   >  1:1398980/1‑109 (MQ=255)

                                                             cgaACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAacgac                                                                                                   <  2:1398980/109‑1 (MQ=255)

                                                                  tGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  2:1111861/92‑1 (MQ=255)

                                                                   gACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACt                                                              <  2:1063220/140‑1 (MQ=255)

                                                                   gACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACt                                                              <  2:857172/140‑1 (MQ=255)

                                                                   gACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACt                                                              <  2:1538875/140‑1 (MQ=255)

                                                                    aCCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACtt                                                             >  1:641606/1‑140 (MQ=255)

                                                                         tctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               <  1:2379679/85‑1 (MQ=255)

                                                                         tctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCt                                                                                                               >  2:2379679/1‑85 (MQ=255)

                                                                         tctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGc                                                        <  2:2046683/140‑1 (MQ=255)

                                                                         tctgtctTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGc                                                        <  2:2298627/140‑1 (MQ=255)

                                                                                tCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTaa                                                 <  1:2286781/140‑1 (MQ=255)

                                                                                       tggtTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGACAGCGCAGTACTTCCTGCCGGGTAAAGCGAtt                                          >  1:365403/1‑140 (MQ=255)

                                                                                          ttCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGtt                                       <  1:1149617/140‑1 (MQ=255)

                                                                                          ttCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGtt                                       <  1:1272545/140‑1 (MQ=255)

                                                                                             tGGATTGGGAAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                    <  2:1769421/140‑1 (MQ=255)

                                                                                             tGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                    <  2:334942/140‑1 (MQ=255)

                                                                                             tGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAg                                    >  2:1294852/1‑140 (MQ=255)

                                                                                                  tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                               <  1:607954/140‑1 (MQ=255)

                                                                                                  tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                               <  2:1050981/140‑1 (MQ=255)

                                                                                                  tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                               <  1:2385138/140‑1 (MQ=255)

                                                                                                  tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                               <  1:2065781/140‑1 (MQ=255)

                                                                                                  tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                               <  1:1786336/140‑1 (MQ=255)

                                                                                                  tCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGCTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGa                               <  2:1234773/140‑1 (MQ=255)

                                                                                                    cGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTCACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAg                             >  2:251214/1‑140 (MQ=255)

                                                                                                    cGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCCGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGGTGGAAg                             >  2:710210/1‑140 (MQ=255)

                                                                                                    cGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                         <  1:2349812/112‑1 (MQ=255)

                                                                                                    cGCAGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTg                                                         >  2:2349812/1‑112 (MQ=255)

                                                                                                       aGAACGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATg                          <  1:2382406/140‑1 (MQ=255)

                                                                                                         aaCGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                        >  2:1917799/1‑140 (MQ=255)

                                                                                                         aaCGTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGc                        >  2:799757/1‑140 (MQ=255)

                                                                                                            gTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                     <  2:1251496/140‑1 (MQ=255)

                                                                                                            gTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                     <  1:2303977/140‑1 (MQ=255)

                                                                                                            gTACCGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTa                     <  1:1072727/140‑1 (MQ=255)

                                                                                                               ccGCTGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAg                  <  2:1938752/140‑1 (MQ=255)

                                                                                                               ccGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAg                  <  2:816368/140‑1 (MQ=255)

                                                                                                                cGCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGa                 >  1:823956/1‑140 (MQ=255)

                                                                                                                 gCAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGAt                <  1:1723961/140‑1 (MQ=255)

                                                                                                                  cAGGTGGTAAAGATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATc               <  2:627235/140‑1 (MQ=255)

                                                                                                                             gATCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGa    <  1:275398/140‑1 (MQ=255)

                                                                                                                              aTCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGac   <  2:484313/140‑1 (MQ=255)

                                                                                                                               tCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGaca  >  1:1369356/1‑140 (MQ=255)

                                                                                                                               tCTGCTTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGaca  >  2:1273069/1‑140 (MQ=255)

TAAGCGGTTTTGTACGCTTTACTGACATGATCGACGGCCAGACCATTACGCGTCAGACCGACGAACTGACCGGTCTGTCTTCGCTGGTGGTTCTGGATTCCGCAGAACGTACCGCAGGTGGTAAAGATCTGCGTCCGGCACTGAAAATCGTTGATGCTCAGGGTAACGACGTTCTGATCCCAGGTACCGATATGCCAGCGCAGTACTTCCTGCCGGGTAAAGCGATTGTTCAGCTGGAAGATGGCGTACAGATCAGCTCTGGTGACA  >  CP009273/4178369‑4178635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A